Construct: ORF TRCN0000492218

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000622.1_s317c1
Derived from:
ccsbBroadEn_07090
DNA Barcode:
CAAACTCGTCTCTACCATCGTAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPM1 (7168)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492218
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7168 TPM1 tropomyosin 1 NM_000366.6 99.7% 100% 180C>T;453C>A
2 human 7168 TPM1 tropomyosin 1 XM_017022538.2 99.7% 100% 180C>T;453C>A
3 human 7168 TPM1 tropomyosin 1 XM_006720667.4 99.6% 99.6% 180C>T;453C>A;852A>G
4 human 7168 TPM1 tropomyosin 1 NM_001365779.1 96% 94.3% (many diffs)
5 human 7168 TPM1 tropomyosin 1 NM_001018005.2 95.9% 94% (many diffs)
6 human 7168 TPM1 tropomyosin 1 XM_017022539.2 94.6% 93.6% (many diffs)
7 human 7168 TPM1 tropomyosin 1 XM_005254639.4 94.2% 90.6% (many diffs)
8 human 7168 TPM1 tropomyosin 1 NM_001018006.2 93.1% 92.2% (many diffs)
9 human 7168 TPM1 tropomyosin 1 NM_001365776.1 91% 88% (many diffs)
10 human 7168 TPM1 tropomyosin 1 NM_001365777.1 90.6% 85% (many diffs)
11 human 7168 TPM1 tropomyosin 1 NM_001018004.2 89.5% 86.6% (many diffs)
12 human 7168 TPM1 tropomyosin 1 NM_001301244.2 86.6% 84.5% (many diffs)
13 human 7168 TPM1 tropomyosin 1 XM_017022536.2 86.6% 78.9% (many diffs)
14 human 7168 TPM1 tropomyosin 1 NM_001018020.2 84.1% 82.7% (many diffs)
15 human 7168 TPM1 tropomyosin 1 XM_017022534.2 83.4% 74% (many diffs)
16 human 7168 TPM1 tropomyosin 1 NM_001365781.2 81% 75.8% (many diffs)
17 human 7168 TPM1 tropomyosin 1 XM_005254650.3 80.9% 75.5% (many diffs)
18 human 7168 TPM1 tropomyosin 1 NM_001018007.2 80.7% 77.1% (many diffs)
19 human 7168 TPM1 tropomyosin 1 XM_017022537.2 78.4% 70.7% (many diffs)
20 human 7168 TPM1 tropomyosin 1 NM_001365778.1 78.3% 75.4% (many diffs)
21 human 7168 TPM1 tropomyosin 1 NM_001365782.1 77.5% 70.3% (many diffs)
22 human 7168 TPM1 tropomyosin 1 NM_001330346.2 77.4% 70% (many diffs)
23 human 7168 TPM1 tropomyosin 1 NM_001330351.2 76.1% 69.6% (many diffs)
24 human 7168 TPM1 tropomyosin 1 XM_017022535.2 75.4% 65.8% (many diffs)
25 human 7168 TPM1 tropomyosin 1 NM_001330344.2 74.9% 68.2% (many diffs)
26 human 7168 TPM1 tropomyosin 1 NM_001018008.2 72.7% 64.1% (many diffs)
27 human 7168 TPM1 tropomyosin 1 NM_001365780.1 72.6% 61.5% (many diffs)
28 human 7168 TPM1 tropomyosin 1 NM_001301289.2 71.6% 62.7% (many diffs)
29 human 7168 TPM1 tropomyosin 1 XM_005254646.2 69.9% 58.3% (many diffs)
30 human 7168 TPM1 tropomyosin 1 XM_017022540.1 66.9% 53.5% (many diffs)
31 human 7168 TPM1 tropomyosin 1 XM_024450040.1 46.8% 40.5% (many diffs)
32 human 7168 TPM1 tropomyosin 1 XR_002957675.1 26.6% (many diffs)
33 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164251.1 94.7% 99.2% (many diffs)
34 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164248.1 91% 93.6% (many diffs)
35 mouse 22003 Tpm1 tropomyosin 1, alpha XM_011242715.2 89.5% 90.2% (many diffs)
36 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164250.1 87.9% 91.9% (many diffs)
37 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511043.3 87.3% 89.4% (many diffs)
38 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511040.3 86% 84.7% (many diffs)
39 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164255.1 85.3% 87.6% (many diffs)
40 mouse 22003 Tpm1 tropomyosin 1, alpha NM_024427.4 84.4% 86.2% (many diffs)
41 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511041.1 83.9% 84.1% (many diffs)
42 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511042.3 83.8% 83.8% (many diffs)
43 mouse 22003 Tpm1 tropomyosin 1, alpha XM_011242714.2 82.4% 80.5% (many diffs)
44 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511046.2 81.6% 83.4% (many diffs)
45 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511044.1 80.8% 82% (many diffs)
46 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511050.2 78.5% 75.5% (many diffs)
47 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511045.2 78.3% 77.8% (many diffs)
48 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164249.1 77.5% 76.4% (many diffs)
49 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164254.1 75% 69.6% (many diffs)
50 mouse 22003 Tpm1 tropomyosin 1, alpha XM_011242716.2 73.6% 66.6% (many diffs)
51 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511051.3 72.9% 69.3% (many diffs)
52 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164253.1 72.1% 67.9% (many diffs)
53 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164256.1 69.6% 63.7% (many diffs)
54 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164252.1 68.9% 62.4% (many diffs)
55 mouse 22003 Tpm1 tropomyosin 1, alpha XM_017313283.1 65.9% 65.1% (many diffs)
56 mouse 22003 Tpm1 tropomyosin 1, alpha XR_379412.3 53.1% (many diffs)
57 mouse 22003 Tpm1 tropomyosin 1, alpha XR_379413.1 37.6% (many diffs)
58 mouse 22003 Tpm1 tropomyosin 1, alpha XR_001778851.1 24% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
918
ORF length:
852
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga cgccatcaag aagaagatgc agatgctgaa gctcgacaag gagaacgcct
121tggatcgagc tgagcaggcg gaggccgaca agaaggcggc ggaagacagg agcaagcagc
181tggaagatga gctggtgtca ctgcaaaaga aactcaaggg caccgaagat gaactggaca
241aatattctga ggctctcaaa gatgcccagg agaagctgga gctggcagag aaaaaggcca
301ccgatgctga agccgacgta gcttctctga acagacgcat ccagctggtt gaggaagagt
361tggatcgtgc ccaggagcgt ctggcaacag ctttgCAGAA GCTGGAGGAA GCTGAGAAGG
421CAGCAGATGA GAGTGAGAGA GGCATGAAAG TCATTGAGAG TCGAGCCCAA AAAGATGAAG
481AAAAAATGGA AATTCAGGAG ATCCAACTGA AAGAGGCAAA GCACATTGCT GAAGATGCCG
541ACCGCAAATA TGAAGAGGTG GCCCGTAAGC TGGTCATCAT TGAGAGCGAC CTGGAACGTG
601CAGAGGAGCG GGCTGAGCTC TCAGAAGGCC AAGTCCGACA GCTGGAAGAA CAATTAAGAA
661TAATGGATCA GACCTTGAAA GCATTAATGG CTGCAGAGGA TAAGTACTCG CAGAAGGAAG
721ACAGATATGA GGAAGAGATC AAGGTCCTTT CCGACAAGCT GAAGGAGGCT GAGACTCGGG
781CTGAGTTTGC GGAGAGGTCA GTAACTAAAT TGGAGAAAAG CATTGATGAC TTAGAAGACG
841AGCTGTACGC TCAGAAACTG AAGTACAAAG CCATCAGCGA GGAGCTGGAC CACGCTCTCA
901ACGATATGAC TTCCATGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
961TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1021GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACAAACTC GTCTCTACCA
1081TCGTAACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.