Construct: ORF TRCN0000492245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007946.1_s317c1
- Derived from:
- ccsbBroadEn_04417
- DNA Barcode:
- TGCCCCCTTGAGTGATGTTAGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPT2 (84706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492245
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84706 | GPT2 | glutamic--pyruvic transamin... | NM_133443.4 | 100% | 100% | |
| 2 | human | 84706 | GPT2 | glutamic--pyruvic transamin... | NM_001142466.3 | 80.8% | 80.8% | 0_1ins300 |
| 3 | human | 84706 | GPT2 | glutamic--pyruvic transamin... | XM_017023790.1 | 72.4% | 71.8% | (many diffs) |
| 4 | mouse | 108682 | Gpt2 | glutamic pyruvate transamin... | NM_173866.3 | 88.4% | 92.9% | (many diffs) |
| 5 | mouse | 108682 | Gpt2 | glutamic pyruvate transamin... | XM_006530559.2 | 48.2% | 45.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1638
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagcgggcg gcggcgctgg tccggcgggg ctgtggtccc cggaccccca 121 gctcctgggg ccgcagccag agcagcgcgg ccgccgaggc ctcggcggtg ctcaaggtgc 181 ggcccgagcg cagccggcgc gagcgcatcc tcacgctgga gtccatgaac ccgcaggtga 241 aggcggtgga gtacgccgtg cggggaccca tcgtgctcaa ggccggcgag atcgagctcg 301 agctgcagcg gggtatcaaa aagccattca cagaggtcat ccgagccaac atcggggacg 361 cccaggctat ggggcagcag ccaatcacct tcctccggca ggtgatggca ctatgcacct 421 acccaaacct gctggacagc cccagcttcc cagaagatgc taagaaacgt gcccggcgga 481 tcctgcaggc ttgtggcggg aacagcctgg ggtcctacag tgctagccag ggtgtcaact 541 gcatccgtga agatgtggct gcctacatca ccaggaggga tggcggtgtg cctgcggacc 601 ccgacaacat ctacctgacc acgggagcta gtgacggcat ttctacgatc ctgaagatcc 661 tcgtctccgg gggcggcaag tcacggacag gtgtgatgat ccccatccca caatatcccc 721 tctattcagc tgtcatctct gagctcgacg ccatccaggt gaattactac ctggacgagg 781 agaactgctg ggcgctgaat gtgaatgagc tccggcgggc ggtgcaggag gccaaagacc 841 actgtgatcc taaggtgctc tgcataatca accctgggaa ccccacaggc caggtacaaa 901 gcagaaagtg catagaagat gtgatccact ttgcctggga agagaagctc tttctcctgg 961 ctgatgaggt gtaccaggac aacgtgtact ctccagattg cagattccac tccttcaaga 1021 aggtgctgta cgagatgggg cccgagtact ccagcaacgt ggagctcgcc tccttccact 1081 ccacctccaa gggctacatg ggcgagtgtg gttacagagg aggctacatg gaggtgatca 1141 acctgcaccc tgagatcaag ggccagctgg tgaagctgct gtcggtgcgc ctgtgccccc 1201 cagtgtctgg gCAGGCCGCC ATGGACATTG TCGTGAACCC CCCGGTGGCA GGAGAGGAGT 1261 CCTTTGAGCA ATTCAGCCGA GAGAAGGAGT CGGTCCTGGG TAATCTGGCC AAAAAAGCAA 1321 AGCTGACGGA AGACCTGTTT AACCAAGTCC CAGGAATTCA CTGCAACCCC TTGCAGGGGG 1381 CCATGTACGC CTTCCCTCGG ATCTTCATTC CTGCCAAAGC TGTGGAGGCT GCTCAGGCCC 1441 ATCAAATGGC TCCAGACATG TTCTACTGCA TGAAGCTCCT GGAGGAGACT GGCATCTGTG 1501 TCGTGCCCGG CAGTGGCTTT GGGCAGAGGG AAGGCACTTA CCACTTCAGG ATGACTATCC 1561 TCCCTCCAGT GGAGAAGCTG AAAACGGTGC TGCAGAAGGT GAAAGACTTC CACATCAACT 1621 TCCTGGAGAA GTACGCGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1681 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1741 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGCCCCC TTGAGTGATG 1801 TTAGTGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt