Construct: ORF TRCN0000492257

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011615.1_s317c1
Derived from:
ccsbBroadEn_03674
DNA Barcode:
CAGAGTAATCATATCCCTTTGTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CBWD1 (55871)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492257
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55871 CBWD1 COBW domain containing 1 NM_018491.3 100% 100%
2 human 150472 CBWD2 COBW domain containing 2 NM_172003.3 98.9% 98.4% (many diffs)
3 human 445571 CBWD3 COBW domain containing 3 NM_201453.3 98.8% 97.9% (many diffs)
4 human 220869 CBWD5 COBW domain containing 5 NM_001330668.1 98.8% 97.7% (many diffs)
5 human 644019 CBWD6 COBW domain containing 6 NM_001085457.1 98.3% 97.2% (many diffs)
6 human 55871 CBWD1 COBW domain containing 1 NM_001145356.1 95.1% 95.1% 704_705ins57
7 human 55871 CBWD1 COBW domain containing 1 XM_005251513.4 94.9% 94.9% 428_429ins60
8 human 150472 CBWD2 COBW domain containing 2 NM_001330336.1 94.1% 93.6% (many diffs)
9 human 445571 CBWD3 COBW domain containing 3 XM_005277637.2 94% 93.1% (many diffs)
10 human 220869 CBWD5 COBW domain containing 5 NM_001363751.1 94% 92.9% (many diffs)
11 human 220869 CBWD5 COBW domain containing 5 XM_005272750.1 93.9% 92.9% (many diffs)
12 human 445571 CBWD3 COBW domain containing 3 NM_001291821.1 93.8% 92.9% (many diffs)
13 human 644019 CBWD6 COBW domain containing 6 XM_017015035.1 93.5% 92.4% (many diffs)
14 human 644019 CBWD6 COBW domain containing 6 XM_017015020.1 93.5% 92.1% (many diffs)
15 human 55871 CBWD1 COBW domain containing 1 XM_005251514.1 92.6% 92.6% 572_573ins87
16 human 150472 CBWD2 COBW domain containing 2 NM_001330337.1 91.8% 91.3% (many diffs)
17 human 445571 CBWD3 COBW domain containing 3 XM_005277639.2 91.8% 90.8% (many diffs)
18 human 220869 CBWD5 COBW domain containing 5 XM_005272751.1 91.8% 90.6% (many diffs)
19 human 644019 CBWD6 COBW domain containing 6 XM_024447653.1 91.5% 90.3% (many diffs)
20 human 644019 CBWD6 COBW domain containing 6 XM_017015019.1 90% 88.7% (many diffs)
21 human 55871 CBWD1 COBW domain containing 1 NM_001145355.1 88.8% 86.7% (many diffs)
22 human 644019 CBWD6 COBW domain containing 6 XM_017015023.1 88.6% 87.3% (many diffs)
23 human 150472 CBWD2 COBW domain containing 2 NM_001330339.1 87.9% 85.5% (many diffs)
24 human 55871 CBWD1 COBW domain containing 1 XM_005251515.1 87.8% 87.8% 572_573ins87;617_618ins57
25 human 150472 CBWD2 COBW domain containing 2 XM_005263604.2 87% 86.5% (many diffs)
26 human 445571 CBWD3 COBW domain containing 3 XM_005277640.2 87% 86% (many diffs)
27 human 220869 CBWD5 COBW domain containing 5 NM_001024916.3 87% 85.8% (many diffs)
28 human 445571 CBWD3 COBW domain containing 3 XM_017014730.1 86.8% 85.8% (many diffs)
29 human 644019 CBWD6 COBW domain containing 6 XM_017015036.1 86.7% 85.5% (many diffs)
30 human 644019 CBWD6 COBW domain containing 6 XM_017015024.1 86.6% 85.3% (many diffs)
31 human 644019 CBWD6 COBW domain containing 6 XM_017015021.1 85.4% 84.1% (many diffs)
32 human 55871 CBWD1 COBW domain containing 1 XM_011517961.2 84% 82% (many diffs)
33 human 644019 CBWD6 COBW domain containing 6 XM_017015022.1 83.4% 82.1% (many diffs)
34 human 55871 CBWD1 COBW domain containing 1 XM_011517962.3 80.3% 80.2% 953_957delCAGAAinsG;960_961ins229
35 human 150472 CBWD2 COBW domain containing 2 XM_011510667.1 79.4% 79.2% (many diffs)
36 human 445571 CBWD3 COBW domain containing 3 XM_006717116.1 79.3% 78.9% (many diffs)
37 human 220869 CBWD5 COBW domain containing 5 XM_006716914.1 79.3% 78.7% (many diffs)
38 human 644019 CBWD6 COBW domain containing 6 XM_017015025.1 78.8% 77.5% (many diffs)
39 human 445571 CBWD3 COBW domain containing 3 XR_001746301.1 77.3% (many diffs)
40 human 220869 CBWD5 COBW domain containing 5 XM_017014455.1 72.4% 71.6% (many diffs)
41 human 644019 CBWD6 COBW domain containing 6 XM_017015026.1 71.4% 70.7% (many diffs)
42 human 445571 CBWD3 COBW domain containing 3 XR_001746302.1 71% (many diffs)
43 human 445571 CBWD3 COBW domain containing 3 XR_001746303.1 69.3% (many diffs)
44 human 220869 CBWD5 COBW domain containing 5 XM_017014456.1 68% 67.3% (many diffs)
45 human 220869 CBWD5 COBW domain containing 5 XM_024447453.1 68% 67.3% (many diffs)
46 human 644019 CBWD6 COBW domain containing 6 XM_017015027.1 67.4% 61.9% (many diffs)
47 human 220869 CBWD5 COBW domain containing 5 XR_001746234.1 65% (many diffs)
48 human 220869 CBWD5 COBW domain containing 5 XR_428504.1 64.9% (many diffs)
49 human 55871 CBWD1 COBW domain containing 1 XR_001746352.1 64.3% 1_106del;812_934del;1415_1842del
50 human 220869 CBWD5 COBW domain containing 5 XR_001746237.2 63.7% (many diffs)
51 human 220869 CBWD5 COBW domain containing 5 XR_001746232.1 63.4% (many diffs)
52 human 150472 CBWD2 COBW domain containing 2 XM_024452717.1 63.2% 63% (many diffs)
53 human 445571 CBWD3 COBW domain containing 3 XR_001746299.1 62.7% (many diffs)
54 human 644019 CBWD6 COBW domain containing 6 XM_017015030.1 62.6% 57.2% (many diffs)
55 human 55871 CBWD1 COBW domain containing 1 XM_011517963.1 62.5% 62.5% 0_1ins444
56 human 220869 CBWD5 COBW domain containing 5 XR_002956769.1 61.7% (many diffs)
57 human 445571 CBWD3 COBW domain containing 3 XR_001746297.1 61.5% (many diffs)
58 human 150472 CBWD2 COBW domain containing 2 NR_138473.1 61.3% (many diffs)
59 human 220869 CBWD5 COBW domain containing 5 XM_017014457.1 61% 60.2% (many diffs)
60 human 55871 CBWD1 COBW domain containing 1 XM_005251517.5 60.6% 59.5% (many diffs)
61 human 644019 CBWD6 COBW domain containing 6 XM_017015028.1 60.4% 59.5% (many diffs)
62 human 55871 CBWD1 COBW domain containing 1 XM_017014925.1 59.4% 59.7% 708_714delGCTCAGC;717_717delCins476
63 human 644019 CBWD6 COBW domain containing 6 XM_017015029.2 59.1% 58.5% (many diffs)
64 human 445571 CBWD3 COBW domain containing 3 XR_001746300.1 58.8% (many diffs)
65 human 220869 CBWD5 COBW domain containing 5 XM_017014458.1 58.5% 58.2% (many diffs)
66 human 220869 CBWD5 COBW domain containing 5 XR_428503.2 58.4% (many diffs)
67 human 150472 CBWD2 COBW domain containing 2 NR_138474.1 58.3% (many diffs)
68 human 644019 CBWD6 COBW domain containing 6 XR_001746360.1 58.2% (many diffs)
69 human 220869 CBWD5 COBW domain containing 5 XR_002956768.1 57.7% (many diffs)
70 human 220869 CBWD5 COBW domain containing 5 XR_001746239.1 56.1% (many diffs)
71 human 445571 CBWD3 COBW domain containing 3 XR_002956784.1 55.8% (many diffs)
72 human 150472 CBWD2 COBW domain containing 2 NM_001330340.1 55% 53.8% (many diffs)
73 human 150472 CBWD2 COBW domain containing 2 NM_001330342.1 55% 53.8% (many diffs)
74 human 220869 CBWD5 COBW domain containing 5 XM_017014459.1 54.9% 53.8% (many diffs)
75 human 445571 CBWD3 COBW domain containing 3 XM_011518706.2 54.8% 53.8% (many diffs)
76 human 220869 CBWD5 COBW domain containing 5 XR_001746240.1 54.8% (many diffs)
77 human 644019 CBWD6 COBW domain containing 6 XM_017015032.1 54.6% 53% (many diffs)
78 human 644019 CBWD6 COBW domain containing 6 XM_024447652.1 54.5% 53.1% (many diffs)
79 human 445571 CBWD3 COBW domain containing 3 XR_929802.1 54.4% (many diffs)
80 human 55871 CBWD1 COBW domain containing 1 XM_011517964.3 53.3% 52.2% (many diffs)
81 human 220869 CBWD5 COBW domain containing 5 XR_001746238.1 53% (many diffs)
82 human 150472 CBWD2 COBW domain containing 2 XR_244814.2 52.5% (many diffs)
83 human 644019 CBWD6 COBW domain containing 6 XM_017015031.1 52.5% 51.2% (many diffs)
84 human 644019 CBWD6 COBW domain containing 6 XR_001746359.1 50.1% (many diffs)
85 human 445571 CBWD3 COBW domain containing 3 XR_929803.1 50.1% (many diffs)
86 human 55871 CBWD1 COBW domain containing 1 XR_001746353.1 49.7% (many diffs)
87 human 445571 CBWD3 COBW domain containing 3 XR_929804.1 48.6% (many diffs)
88 human 150472 CBWD2 COBW domain containing 2 XR_922864.1 48.1% (many diffs)
89 human 220869 CBWD5 COBW domain containing 5 XM_017014461.1 47.9% 46.8% (many diffs)
90 human 445571 CBWD3 COBW domain containing 3 XM_017014732.1 47.9% 46.8% (many diffs)
91 human 445571 CBWD3 COBW domain containing 3 XR_001746298.1 47.3% (many diffs)
92 human 150472 CBWD2 COBW domain containing 2 XR_427073.2 46.9% (many diffs)
93 human 644019 CBWD6 COBW domain containing 6 XR_001746361.1 46.2% (many diffs)
94 human 644019 CBWD6 COBW domain containing 6 XM_017015033.1 46% 44.7% (many diffs)
95 human 445571 CBWD3 COBW domain containing 3 XR_001746304.1 45.8% (many diffs)
96 human 220869 CBWD5 COBW domain containing 5 XR_001746241.2 45% (many diffs)
97 human 644019 CBWD6 COBW domain containing 6 XR_001746362.1 44.5% (many diffs)
98 human 220869 CBWD5 COBW domain containing 5 NM_001286835.1 43.5% 39.1% (many diffs)
99 human 644019 CBWD6 COBW domain containing 6 XR_001746363.1 43.3% (many diffs)
100 human 150472 CBWD2 COBW domain containing 2 XR_002959242.1 37.8% (many diffs)
101 human 644019 CBWD6 COBW domain containing 6 XM_017015034.1 31.8% 29.8% (many diffs)
102 human 220869 CBWD5 COBW domain containing 5 NM_001286836.1 28.3% 28.1% (many diffs)
103 human 220869 CBWD5 COBW domain containing 5 XR_002956766.1 26.2% (many diffs)
104 human 220869 CBWD5 COBW domain containing 5 XR_001746233.1 25.9% (many diffs)
105 human 220869 CBWD5 COBW domain containing 5 XR_001746235.1 25.8% (many diffs)
106 human 220869 CBWD5 COBW domain containing 5 XR_001746231.1 25.5% (many diffs)
107 human 220869 CBWD5 COBW domain containing 5 XR_002956767.1 22.6% (many diffs)
108 mouse 226043 Cbwd1 COBW domain containing 1 NM_146097.3 83.8% 80.6% (many diffs)
109 mouse 226043 Cbwd1 COBW domain containing 1 NR_033744.1 49.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1251
ORF length:
1185
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt accggctgtt ggatctgcgg atgaggagga ggatcctgcg gaggaggatt
121gtcctgaatt ggttcccatt gagacgacgc aaagcgagga ggaggaaaag tctggcctcg
181gcgccaagat cccagtcaca attatcaccg ggtatttagg tgctgggaag acaacacttc
241tgaactatat tttgacagag caacatagta aaagagtagc ggtcatttta aatgaatttg
301gggaaggaag tgcgctggag aaatccttag ctgtcagcca aggtggagag ctctatgaag
361agtggctgga acttagaaac ggttgcctct gctgttcagt gaaggacagt ggccttagag
421ctattgagaa tttgatgcaa aagaagggga aatttgatta catactgtta gagaccactg
481gattagcaga ccctggtgca gtggcttcta tgttttgggt tgatgctgaa ttagggagtg
541atatttatct tgatggtatc ataactattg tggattcaaa atatggatta aaacatttaa
601cagaagagaa acctgatggc cttatcaatg aagctactag acaagttgct ttggcagatg
661ccattctcat taataaaaca gacctggttc cagaagaaga tgtaaagaaa ttaagaacga
721caattagatc cataaatgga ctaggacaaa tcttagaaac acaaagatca agagttgatc
781tctctaatgt attagatctt catgcctttg atagtctctc tggaaTAAGT TTGCAGAAAA
841AACTTCAGCA TGTGCCAGGA ACACAACCTC ACCTTGATCA GAGTATTGTT ACAATCACAT
901TTGAAGTACC AGGAAATGCA AAGGAAGAAC ATCTTAATAT GTTTATTCAG AATCTCCTGT
961GGGAAAAGAA TGTGAGAAAC AAGGACAATC ACTGCATGGA GGTCATAAGG CTGAAGGGAT
1021TGGTGTCAAT CAAAGACAAA TCACAACAAG TGATTGTCCA GGGTGTCCAT GAGCTCTATG
1081ATCTGGAGGA GACTCCAGTG AGCTGGAAGG ATGACACTGA GAGAACAAAT CGATTGGTCC
1141TCCTTGGCAG AAATTTAGAT AAGGATATCC TTAAACAGCT GTTTATAGCT ACTGTGACAG
1201AAACAGAAAA GCAGTGGACA ACACGTTTCC AAGAAGATCA AGTTTGTACA TACCCAACTT
1261TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1321CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1381CTTGTGGAAA GGACGACAGA GTAATCATAT CCCTTTGTTC ACGCGTTAAG TCgacaatca
1441acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)