Transcript: Mouse NM_146097.3

Mus musculus COBW domain containing 1 (Cbwd1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Cbwd1 (226043)
Length:
1944
CDS:
86..1267

Additional Resources:

NCBI RefSeq record:
NM_146097.3
NBCI Gene record:
Cbwd1 (226043)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_146097.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197585 CTATGATATTCTCTCTGGAAT pLKO.1 820 CDS 100% 4.950 6.930 N Cbwd1 n/a
2 TRCN0000177295 GCTATAGAGAATCTGATGCAA pLKO.1 434 CDS 100% 3.000 2.400 N Cbwd1 n/a
3 TRCN0000176700 CAGACATGATTCTCATCAATA pLKO.1 669 CDS 100% 13.200 9.240 N Cbwd1 n/a
4 TRCN0000215755 GAACAACTATCAGATCAATAA pLKO.1 729 CDS 100% 13.200 9.240 N Cbwd1 n/a
5 TRCN0000197919 GCAGACATGATTCTCATCAAT pLKO.1 668 CDS 100% 5.625 3.938 N Cbwd1 n/a
6 TRCN0000198419 GCTGGTCTTCATTGGTAGAAA pLKO.1 1144 CDS 100% 5.625 3.938 N Cbwd1 n/a
7 TRCN0000177047 GAGTATTGTTACAGTCACATT pLKO.1 892 CDS 100% 4.950 3.465 N Cbwd1 n/a
8 TRCN0000200027 CCCATTGAGACCAAGAACCAA pLKO.1 152 CDS 100% 3.000 2.100 N Cbwd1 n/a
9 TRCN0000177842 CTAGTGAACTGGAAGGATGAT pLKO.1 1106 CDS 100% 0.000 0.000 N Cbwd1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_146097.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10172 pDONR223 100% 84.1% 80.3% None (many diffs) n/a
2 ccsbBroad304_10172 pLX_304 0% 84.1% 80.3% V5 (many diffs) n/a
3 ccsbBroadEn_08604 pDONR223 100% 84% 80.6% None (many diffs) n/a
4 ccsbBroad304_08604 pLX_304 0% 84% 80.6% V5 (many diffs) n/a
5 ccsbBroadEn_03674 pDONR223 100% 83.8% 80.6% None (many diffs) n/a
6 ccsbBroad304_03674 pLX_304 0% 83.8% 80.6% V5 (many diffs) n/a
7 TRCN0000492257 CAGAGTAATCATATCCCTTTGTTC pLX_317 36.1% 83.8% 80.6% V5 (many diffs) n/a
8 ccsbBroadEn_08603 pDONR223 100% 75.9% 73.1% None (many diffs) n/a
9 ccsbBroad304_08603 pLX_304 0% 75.9% 73.1% V5 (many diffs) n/a
10 TRCN0000491579 GTTCACCCGAGATAGGATGAAGAC pLX_317 32.7% 75.9% 73.1% V5 (many diffs) n/a
11 ccsbBroadEn_13415 pDONR223 100% 39.2% 37.4% None (many diffs) n/a
12 ccsbBroad304_13415 pLX_304 0% 39.2% 37.4% V5 (many diffs) n/a
13 TRCN0000480607 CTTTTTTTTCAGTAGCCCCGCCGA pLX_317 73.5% 39.2% 37.4% V5 (many diffs) n/a
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