Construct: ORF TRCN0000492284

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020698.2_s317c1
DNA Barcode:
AATCCATTCTCGATCTTGGTCAAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PRPF4B (8899)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492284
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8899 PRPF4B pre-mRNA processing factor 4B NM_003913.5 99.9% 99.9% 247A>G
2 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011410.2 99.9% 99.9% 247A>G
3 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011411.2 99.9% 99.9% 247A>G
4 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011413.2 99.9% 99.9% 247A>G
5 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011414.2 99.9% 99.9% 247A>G
6 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011415.2 99.9% 99.9% 247A>G
7 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011416.2 99.9% 99.9% 247A>G
8 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011417.2 99.9% 99.9% 247A>G
9 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011418.2 99.9% 99.9% 247A>G
10 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011420.2 99.9% 99.9% 247A>G
11 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011421.2 99.9% 99.9% 247A>G
12 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011422.2 99.9% 99.9% 247A>G
13 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011423.2 99.9% 99.9% 247A>G
14 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011424.2 99.9% 99.9% 247A>G
15 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011425.2 99.9% 99.9% 247A>G
16 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011426.2 99.9% 99.9% 247A>G
17 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011427.2 99.9% 99.9% 247A>G
18 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011428.2 99.9% 99.9% 247A>G
19 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011430.2 99.9% 99.9% 247A>G
20 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011432.2 85.6% 85.3% (many diffs)
21 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011433.2 85.6% 85.3% (many diffs)
22 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011434.2 85.6% 85.3% (many diffs)
23 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011435.2 85.6% 85.3% (many diffs)
24 human 8899 PRPF4B pre-mRNA processing factor 4B NR_146784.2 84.3% 1_126del;373A>G;3148_3582del
25 human 8899 PRPF4B pre-mRNA processing factor 4B XM_011514970.3 81.8% 81.6% (many diffs)
26 human 8899 PRPF4B pre-mRNA processing factor 4B NR_146783.2 81.2% 1_126del;373A>G;3148_3715del
27 human 8899 PRPF4B pre-mRNA processing factor 4B NR_146785.2 79.6% 1_126del;373A>G;3148_3792del
28 mouse 19134 Prpf4b pre-mRNA processing factor 4B NM_013830.2 88.9% 95.1% (many diffs)
29 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315425.1 88.9% 95.1% (many diffs)
30 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315426.1 88.9% 95.1% (many diffs)
31 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315427.1 88.9% 95.1% (many diffs)
32 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315428.1 88.9% 95.1% (many diffs)
33 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315429.1 88.9% 95.1% (many diffs)
34 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315430.1 88.9% 95.1% (many diffs)
35 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315431.1 88.9% 95.1% (many diffs)
36 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315432.1 88.9% 95.1% (many diffs)
37 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315433.1 88.9% 95.1% (many diffs)
38 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315434.1 88.9% 95.1% (many diffs)
39 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315435.1 88.9% 95.1% (many diffs)
40 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315436.1 88.9% 95.1% (many diffs)
41 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315437.1 88.9% 95.1% (many diffs)
42 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_006516582.3 77.5% 79.8% (many diffs)
43 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_006516583.3 77.4% 79.9% (many diffs)
44 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_006516584.3 77.1% 80.3% (many diffs)
45 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315438.1 77.1% 80.3% (many diffs)
46 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315439.1 56.1% 59.7% (many diffs)
47 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315440.1 56.1% 59.7% (many diffs)
48 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315441.1 54.7% 56.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3093
ORF length:
3021
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggccgcc gcggagaccc agtcgctacg ggagcagcca gagatggaag
121atgctaattc tgaaaagagt ataaatgaag aaaatggaga agtatcagaa gaccagtctc
181aaaataagca cagtcgtcac aaaaaaaaga agcataaaca cagaagtaaa cataagaaac
241ataaacattc ctcagaagaa gacaaggata aaaaacataa acataagcat aaacataaga
301aacacaaaag aaaagaggtt attgatgctt ctgataaaga gggtatgtct ccagcaaaaa
361gaactaaact tgatgattta gctttgctag aagacttgga aaaacagaga gccttgatta
421aggccgaact tgataatgag ttaatggaag gaaaggtcca gtctggtatg gggctcattt
481tgcaaggtta tgagtctggc tctgaagaag agggggaaat tcatgaaaag gcaagaaatg
541gaaataggtc tagtactaga tcttcaagta caaaggggaa acttgaactt gtggacaata
601aaattactac aaagaaacga agtaaaagca gatccaaaga acggactaga cataggtctg
661ataaaaagaa aagtaagggg ggtattgaaa tcgttaaaga gaaaacaact aggagcaagt
721caaaggagag gaaaaaatct aaaagcccat ccaaaagaag taagtctcaa gatcaagcaa
781ggaaatcaaa atcccctacc cttagaaggc gatctcaaga gaaaattggt aaggccagat
841ctcctactga tgataaggtt aaaattgaag ataaaagtaa atcaaaagat aggaaaaaat
901ccccaattat aaatgaaagt agaagtcgcg atcgaggtaa aaaatccaga tccccagttg
961atttaagagg taaatccaaa gacagaaggt cacggtccaa agagagaaaa tcaaaacggt
1021ctgaaactga taaagaaaag aagccaatta aatctccctc taaagatgct tcatctggga
1081aagaaaatag gtcacccagc agaagacctg gtcgtagtcc taaaagaaga agtttgtctc
1141caaaaccacg tgataaatca agaagaagca ggtctccact tttgaatgat agaagatcta
1201agcagagcaa atccccctcg cggacactgt ctcctgggag aagagccaag agccgatcct
1261tagaaagaaa acgacgagaa ccagagagga gacgactttc ttctccaaga acacgacctc
1321gagatgatat cctcagtaga cgtgaaagat caaaagatgc cagccccatc aatagatggt
1381ctccaacccg aagaagaagt agatctccca ttagaaggag gtctcgttcc ccactcagac
1441gtagcaggtc tccaagaaga agaagcagat ctcctcggag aagggacaga ggtcggagga
1501gcagatcacg cttgcgaagg cggtctcgat cacgcggtgg tcgtagacga aggagcagaa
1561gcaaagtaaa ggaagataaa tttaaaggaa gtctttctga aggaatgaaa gttgagcagg
1621aatcttcgtc tgatgataac cttgaagact ttgatgtaga ggaagaagat gaagaagccc
1681taatagaaca gagaagaatc caaaggcagg caattgttca gaaatataaa taccttgctg
1741aagatagcaa catgtctgtg ccatctgaac caagcagccc ccagagcagt acgagaacac
1801gatcaccatc tccagatgac attctggagc gagtagctgc tgatgttaaa gagtatgaac
1861gggaaaatgt tgatacattt gaggcctcag tgaaagccaa gcataatcta atgacagttg
1921aacagaataa tggttcatct cagaagaagt tgttggcacc tgatatgttt acagaatctg
1981atgatatgtt tgctgcgtat tttgatagtg ctcgtcttcg ggccgctggc attggaaaag
2041atttcaaaga gaatcccaac ctcagagata actggaccga tgcagaaggc tattatcgtg
2101tgaacatagg tgaagtccta gataaacgtt acaatgtgta tggctacact gggcaaggtg
2161tattcagtaa tgttgtacga gccagagata atgcaagagc caaccaagaa gtggctgtaa
2221agatcatcag aaacaatgag ctcatgcaaa agactggttt aaaagaatta gagttcttga
2281aaaaacttaa tgatgctgat cctgatgaca aatttcattg tctgagactc ttcaggcact
2341tctatcacaa gcagcatctt tgtctggtat tcgagcctct cagcatgaac ttacgagagg
2401tgttaaaaaa atatggtaaa gatgttggtc ttcatattaa agctgtaaga tcctatagtc
2461agcagttgtt cctggcattg aaactcctta aaagatgcaa tatcctacat gcagatatca
2521agccagacaa tatcctggtt aatgaatcca aaactatttt aaagctttgc gattttgggt
2581cggcttcaca tgttgcggat aatgacataa caccttatct tgtcagtaga ttttatcgtg
2641ctcctgaaat cattataggt aaaagctatg actatggtat agatatgtgg tctgtaggtt
2701gcaccttata cgaactctat actggaaaaa ttttattccc tggcaaaacc aataaccata
2761tgcTGAAGCT TGCAATGGAT CTCAAAGGAA AGATGCCAAA TAAGATGATT CGAAAAGGTG
2821TGTTCAAAGA TCAGCATTTT GATCAAAATC TCAACTTCAT GTACATAGAA GTTGATAAAG
2881TAACAGAGAG GGAGAAAGTT ACTGTTATGA GCACCATTAA TCCAACTAAG GACCTGTTGG
2941CTGACTTGAT TGGGTGCCAG AGACTTCCTG AAGACCAACG TAAGAAAGTA CACCAGCTAA
3001AGGACTTGTT GGACCAGATT CTGATGTTGG ACCCAGCTAA ACGAATTAGC ATCAACCAGG
3061CCCTACAGCA CGCCTTCATC CAGGAAAAAA TTTAGAACCC AGCTTTCTTG TACAAAGTGG
3121TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
3181CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
3241AAATCCATTC TCGATCTTGG TCAACACGCG TTAAGTCgac aatcaacctc tggattacaa
3301aatttgtgaa agatt

Download FASTA (ORF) (Full)

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