Construct: ORF TRCN0000492284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020698.2_s317c1
- DNA Barcode:
- AATCCATTCTCGATCTTGGTCAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRPF4B (8899)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492284
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NM_003913.5 | 99.9% | 99.9% | 247A>G |
| 2 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011410.2 | 99.9% | 99.9% | 247A>G |
| 3 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011411.2 | 99.9% | 99.9% | 247A>G |
| 4 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011413.2 | 99.9% | 99.9% | 247A>G |
| 5 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011414.2 | 99.9% | 99.9% | 247A>G |
| 6 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011415.2 | 99.9% | 99.9% | 247A>G |
| 7 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011416.2 | 99.9% | 99.9% | 247A>G |
| 8 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011417.2 | 99.9% | 99.9% | 247A>G |
| 9 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011418.2 | 99.9% | 99.9% | 247A>G |
| 10 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011420.2 | 99.9% | 99.9% | 247A>G |
| 11 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011421.2 | 99.9% | 99.9% | 247A>G |
| 12 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011422.2 | 99.9% | 99.9% | 247A>G |
| 13 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011423.2 | 99.9% | 99.9% | 247A>G |
| 14 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011424.2 | 99.9% | 99.9% | 247A>G |
| 15 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011425.2 | 99.9% | 99.9% | 247A>G |
| 16 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011426.2 | 99.9% | 99.9% | 247A>G |
| 17 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011427.2 | 99.9% | 99.9% | 247A>G |
| 18 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011428.2 | 99.9% | 99.9% | 247A>G |
| 19 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011430.2 | 99.9% | 99.9% | 247A>G |
| 20 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011432.2 | 85.6% | 85.3% | (many diffs) |
| 21 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011433.2 | 85.6% | 85.3% | (many diffs) |
| 22 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011434.2 | 85.6% | 85.3% | (many diffs) |
| 23 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011435.2 | 85.6% | 85.3% | (many diffs) |
| 24 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146784.2 | 84.3% | 1_126del;373A>G;3148_3582del | |
| 25 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_011514970.3 | 81.8% | 81.6% | (many diffs) |
| 26 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146783.2 | 81.2% | 1_126del;373A>G;3148_3715del | |
| 27 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146785.2 | 79.6% | 1_126del;373A>G;3148_3792del | |
| 28 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | NM_013830.2 | 88.9% | 95.1% | (many diffs) |
| 29 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315425.1 | 88.9% | 95.1% | (many diffs) |
| 30 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315426.1 | 88.9% | 95.1% | (many diffs) |
| 31 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315427.1 | 88.9% | 95.1% | (many diffs) |
| 32 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315428.1 | 88.9% | 95.1% | (many diffs) |
| 33 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315429.1 | 88.9% | 95.1% | (many diffs) |
| 34 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315430.1 | 88.9% | 95.1% | (many diffs) |
| 35 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315431.1 | 88.9% | 95.1% | (many diffs) |
| 36 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315432.1 | 88.9% | 95.1% | (many diffs) |
| 37 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315433.1 | 88.9% | 95.1% | (many diffs) |
| 38 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315434.1 | 88.9% | 95.1% | (many diffs) |
| 39 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315435.1 | 88.9% | 95.1% | (many diffs) |
| 40 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315436.1 | 88.9% | 95.1% | (many diffs) |
| 41 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315437.1 | 88.9% | 95.1% | (many diffs) |
| 42 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_006516582.3 | 77.5% | 79.8% | (many diffs) |
| 43 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_006516583.3 | 77.4% | 79.9% | (many diffs) |
| 44 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_006516584.3 | 77.1% | 80.3% | (many diffs) |
| 45 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315438.1 | 77.1% | 80.3% | (many diffs) |
| 46 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315439.1 | 56.1% | 59.7% | (many diffs) |
| 47 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315440.1 | 56.1% | 59.7% | (many diffs) |
| 48 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315441.1 | 54.7% | 56.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3093
- ORF length:
- 3021
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgcc gcggagaccc agtcgctacg ggagcagcca gagatggaag 121 atgctaattc tgaaaagagt ataaatgaag aaaatggaga agtatcagaa gaccagtctc 181 aaaataagca cagtcgtcac aaaaaaaaga agcataaaca cagaagtaaa cataagaaac 241 ataaacattc ctcagaagaa gacaaggata aaaaacataa acataagcat aaacataaga 301 aacacaaaag aaaagaggtt attgatgctt ctgataaaga gggtatgtct ccagcaaaaa 361 gaactaaact tgatgattta gctttgctag aagacttgga aaaacagaga gccttgatta 421 aggccgaact tgataatgag ttaatggaag gaaaggtcca gtctggtatg gggctcattt 481 tgcaaggtta tgagtctggc tctgaagaag agggggaaat tcatgaaaag gcaagaaatg 541 gaaataggtc tagtactaga tcttcaagta caaaggggaa acttgaactt gtggacaata 601 aaattactac aaagaaacga agtaaaagca gatccaaaga acggactaga cataggtctg 661 ataaaaagaa aagtaagggg ggtattgaaa tcgttaaaga gaaaacaact aggagcaagt 721 caaaggagag gaaaaaatct aaaagcccat ccaaaagaag taagtctcaa gatcaagcaa 781 ggaaatcaaa atcccctacc cttagaaggc gatctcaaga gaaaattggt aaggccagat 841 ctcctactga tgataaggtt aaaattgaag ataaaagtaa atcaaaagat aggaaaaaat 901 ccccaattat aaatgaaagt agaagtcgcg atcgaggtaa aaaatccaga tccccagttg 961 atttaagagg taaatccaaa gacagaaggt cacggtccaa agagagaaaa tcaaaacggt 1021 ctgaaactga taaagaaaag aagccaatta aatctccctc taaagatgct tcatctggga 1081 aagaaaatag gtcacccagc agaagacctg gtcgtagtcc taaaagaaga agtttgtctc 1141 caaaaccacg tgataaatca agaagaagca ggtctccact tttgaatgat agaagatcta 1201 agcagagcaa atccccctcg cggacactgt ctcctgggag aagagccaag agccgatcct 1261 tagaaagaaa acgacgagaa ccagagagga gacgactttc ttctccaaga acacgacctc 1321 gagatgatat cctcagtaga cgtgaaagat caaaagatgc cagccccatc aatagatggt 1381 ctccaacccg aagaagaagt agatctccca ttagaaggag gtctcgttcc ccactcagac 1441 gtagcaggtc tccaagaaga agaagcagat ctcctcggag aagggacaga ggtcggagga 1501 gcagatcacg cttgcgaagg cggtctcgat cacgcggtgg tcgtagacga aggagcagaa 1561 gcaaagtaaa ggaagataaa tttaaaggaa gtctttctga aggaatgaaa gttgagcagg 1621 aatcttcgtc tgatgataac cttgaagact ttgatgtaga ggaagaagat gaagaagccc 1681 taatagaaca gagaagaatc caaaggcagg caattgttca gaaatataaa taccttgctg 1741 aagatagcaa catgtctgtg ccatctgaac caagcagccc ccagagcagt acgagaacac 1801 gatcaccatc tccagatgac attctggagc gagtagctgc tgatgttaaa gagtatgaac 1861 gggaaaatgt tgatacattt gaggcctcag tgaaagccaa gcataatcta atgacagttg 1921 aacagaataa tggttcatct cagaagaagt tgttggcacc tgatatgttt acagaatctg 1981 atgatatgtt tgctgcgtat tttgatagtg ctcgtcttcg ggccgctggc attggaaaag 2041 atttcaaaga gaatcccaac ctcagagata actggaccga tgcagaaggc tattatcgtg 2101 tgaacatagg tgaagtccta gataaacgtt acaatgtgta tggctacact gggcaaggtg 2161 tattcagtaa tgttgtacga gccagagata atgcaagagc caaccaagaa gtggctgtaa 2221 agatcatcag aaacaatgag ctcatgcaaa agactggttt aaaagaatta gagttcttga 2281 aaaaacttaa tgatgctgat cctgatgaca aatttcattg tctgagactc ttcaggcact 2341 tctatcacaa gcagcatctt tgtctggtat tcgagcctct cagcatgaac ttacgagagg 2401 tgttaaaaaa atatggtaaa gatgttggtc ttcatattaa agctgtaaga tcctatagtc 2461 agcagttgtt cctggcattg aaactcctta aaagatgcaa tatcctacat gcagatatca 2521 agccagacaa tatcctggtt aatgaatcca aaactatttt aaagctttgc gattttgggt 2581 cggcttcaca tgttgcggat aatgacataa caccttatct tgtcagtaga ttttatcgtg 2641 ctcctgaaat cattataggt aaaagctatg actatggtat agatatgtgg tctgtaggtt 2701 gcaccttata cgaactctat actggaaaaa ttttattccc tggcaaaacc aataaccata 2761 tgcTGAAGCT TGCAATGGAT CTCAAAGGAA AGATGCCAAA TAAGATGATT CGAAAAGGTG 2821 TGTTCAAAGA TCAGCATTTT GATCAAAATC TCAACTTCAT GTACATAGAA GTTGATAAAG 2881 TAACAGAGAG GGAGAAAGTT ACTGTTATGA GCACCATTAA TCCAACTAAG GACCTGTTGG 2941 CTGACTTGAT TGGGTGCCAG AGACTTCCTG AAGACCAACG TAAGAAAGTA CACCAGCTAA 3001 AGGACTTGTT GGACCAGATT CTGATGTTGG ACCCAGCTAA ACGAATTAGC ATCAACCAGG 3061 CCCTACAGCA CGCCTTCATC CAGGAAAAAA TTTAGAACCC AGCTTTCTTG TACAAAGTGG 3121 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3181 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3241 AAATCCATTC TCGATCTTGG TCAACACGCG TTAAGTCgac aatcaacctc tggattacaa 3301 aatttgtgaa agatt