Construct: ORF ccsbBroad304_00154

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007539.1_s304c1, BRDN0000404802
Derived from:
ccsbBroadEn_00154
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BCKDHB (594)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00154
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 594 BCKDHB branched chain keto acid de... NM_000056.4 100% 100%
2 human 594 BCKDHB branched chain keto acid de... NM_183050.4 100% 100%
3 human 594 BCKDHB branched chain keto acid de... XM_011536023.3 90.8% 89.5% (many diffs)
4 human 594 BCKDHB branched chain keto acid de... XM_005248756.5 90.6% 87.6% (many diffs)
5 human 594 BCKDHB branched chain keto acid de... XM_011536024.3 89% 87.1% (many diffs)
6 human 594 BCKDHB branched chain keto acid de... XM_011536025.3 88.7% 88.5% 1040_1043delGTCTinsAGGA;1045C>T;1050_1050delTins127
7 human 594 BCKDHB branched chain keto acid de... NM_001318975.1 82.1% 82.1% 0_1ins210
8 human 594 BCKDHB branched chain keto acid de... NR_134945.2 31.2% 1_23del;655_748del;1294_3762del
9 human 594 BCKDHB branched chain keto acid de... XR_001743546.2 24.3% (many diffs)
10 human 594 BCKDHB branched chain keto acid de... XR_002956292.1 14.8% (many diffs)
11 human 594 BCKDHB branched chain keto acid de... XR_001743549.2 14.6% (many diffs)
12 human 594 BCKDHB branched chain keto acid de... XR_001743547.2 14.6% (many diffs)
13 human 594 BCKDHB branched chain keto acid de... XR_001743548.2 11% (many diffs)
14 mouse 12040 Bckdhb branched chain ketoacid deh... NM_001305935.1 84.9% 91% (many diffs)
15 mouse 12040 Bckdhb branched chain ketoacid deh... XM_006510786.1 74.2% 79.3% (many diffs)
16 mouse 12040 Bckdhb branched chain ketoacid deh... NM_199195.1 72.6% 79.8% (many diffs)
17 mouse 12040 Bckdhb branched chain ketoacid deh... XM_017313114.1 63.1% 69.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1242
ORF length:
1176
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggttgtagcg gcggctgccg gctggctact caggctcagg gcggcagggg
121ctgaggggca ctggcgtcgg cttcctggcg cggggctggc gcggggcttt ttgcaccccg
181ccgcgactgt cgaggatgcg gcccagaggc ggcaggtggc tcattttact ttccagccag
241atccggagcc ccgggagtac gggcaaactc agaaaatgaa tcttttccag tctgtaacaa
301gtgccttgga taactcattg gccaaagatc ctactgcagt aatatttggt gaagatgttg
361cctttggtgg agtctttaga tgcactgttg gcttgcgaga caaatatgga aaagatagag
421tttttaatac cccattgtgt gaacaaggaa ttgttggatt tggaatcgga attgcggtca
481ctggagctac tgccattgcg gaaattcagt ttgcagatta tattttccct gcatttgatc
541agattgttaa tgaagctgcc aagtatcgct atcgctctgg ggatcttttt aactgtggaa
601gcctcactat ccggtcccct tggggctgtg ttggtcatgg ggctctctat cattctcaga
661gtcctgaagc attttttgcc cattgcccag gaatcaaggt ggttataccc agaagccctt
721tccaggccaa aggacttctt ttgtcatgca tagaggataa aaatccttgt atattttttg
781aacctaaaat actttacagg gcagcagcgg aagaagtccc tatagaacca tacaacatcc
841cactgtccca ggccgaagtc atacaggaag ggagtgatgt tactctagtt gcctggggca
901ctcaggttca tgtgatccga gaggtagctt ccatggcaaa agaaaagctt ggagtgtctt
961gtgaagtcat tgatctgagg actataatac cttgggatgt ggacacaatt tgtaagtctg
1021tgatcaaaac agggcgactg ctaatcagtc acgaggctcc cttgacaggc ggctttgcat
1081cggaaatcag ctctacagtt caggaggaat gtttcttgaa cctagaggct cctatatcaa
1141gagtatgtgg ttatgacaca ccatttcctc acatttttga accattctac atcccagaca
1201aatggaagtg ttatgatgcc cttcgaaaaa tgatcaacta ttgcccaact ttcttgtaca
1261aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1321ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)