Construct: ORF ccsbBroad304_00202
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014303.1_s304c1, BRDN0000461554
- Derived from:
- ccsbBroadEn_00202
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACNB4 (785)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00202
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 785 | CACNB4 | calcium voltage-gated chann... | NM_000726.4 | 100% | 100% | |
2 | human | 785 | CACNB4 | calcium voltage-gated chann... | NM_001005746.4 | 95.6% | 95.9% | 0_1ins61;2_8delTGGATGT |
3 | human | 785 | CACNB4 | calcium voltage-gated chann... | NM_001005747.4 | 91.4% | 89.7% | (many diffs) |
4 | human | 785 | CACNB4 | calcium voltage-gated chann... | NM_001320722.3 | 90.8% | 90.5% | 0_1ins109;6_6delTins33 |
5 | human | 785 | CACNB4 | calcium voltage-gated chann... | NM_001330118.1 | 90.8% | 90.5% | 0_1ins109;6_6delTins33 |
6 | human | 785 | CACNB4 | calcium voltage-gated chann... | XM_011511796.2 | 90.7% | 90.5% | 0_1ins135;2_3insCCTCAGACA |
7 | human | 785 | CACNB4 | calcium voltage-gated chann... | NM_001145798.2 | 88% | 88% | 1116_1117ins186 |
8 | human | 785 | CACNB4 | calcium voltage-gated chann... | XM_011511797.3 | 87.8% | 84.2% | 597_598insGCAAAACAGAAGCAAAAAGT;677_678ins169 |
9 | human | 785 | CACNB4 | calcium voltage-gated chann... | NM_001330113.2 | 83.5% | 80.2% | (many diffs) |
10 | human | 785 | CACNB4 | calcium voltage-gated chann... | NM_001330115.2 | 79.4% | 74.1% | (many diffs) |
11 | human | 785 | CACNB4 | calcium voltage-gated chann... | NM_001330116.2 | 78.7% | 74.8% | (many diffs) |
12 | human | 785 | CACNB4 | calcium voltage-gated chann... | NM_001330117.2 | 64.2% | 64.2% | 0_1ins558 |
13 | human | 785 | CACNB4 | calcium voltage-gated chann... | XM_024453128.1 | 64.2% | 64.2% | 0_1ins558 |
14 | human | 785 | CACNB4 | calcium voltage-gated chann... | NM_001330114.2 | 58% | 58% | 0_1ins654 |
15 | human | 785 | CACNB4 | calcium voltage-gated chann... | XM_017004885.1 | 58% | 58% | 0_1ins654 |
16 | human | 785 | CACNB4 | calcium voltage-gated chann... | XR_923022.3 | 47.6% | 1_153del;1454_1525del;1786_3271del | |
17 | human | 785 | CACNB4 | calcium voltage-gated chann... | XR_002959337.1 | 45.4% | 1_153del;1454_1688del;1949_3434del | |
18 | human | 785 | CACNB4 | calcium voltage-gated chann... | XR_002959338.1 | 44.2% | (many diffs) | |
19 | human | 785 | CACNB4 | calcium voltage-gated chann... | XM_017004888.2 | 40.5% | 33.8% | (many diffs) |
20 | human | 785 | CACNB4 | calcium voltage-gated chann... | XR_001738938.2 | 39.2% | (many diffs) | |
21 | human | 785 | CACNB4 | calcium voltage-gated chann... | XR_001738928.1 | 30.6% | (many diffs) | |
22 | human | 785 | CACNB4 | calcium voltage-gated chann... | XR_001738939.1 | 17.9% | (many diffs) | |
23 | human | 785 | CACNB4 | calcium voltage-gated chann... | XR_001738940.2 | 17.6% | (many diffs) | |
24 | human | 785 | CACNB4 | calcium voltage-gated chann... | XR_001738935.1 | 16.5% | (many diffs) | |
25 | human | 785 | CACNB4 | calcium voltage-gated chann... | XR_001738937.2 | 16.3% | (many diffs) | |
26 | mouse | 12298 | Cacnb4 | calcium channel, voltage-de... | NM_001037099.2 | 89.9% | 98.2% | (many diffs) |
27 | mouse | 12298 | Cacnb4 | calcium channel, voltage-de... | NM_001285426.1 | 86.2% | 95.1% | (many diffs) |
28 | mouse | 12298 | Cacnb4 | calcium channel, voltage-de... | NM_146123.3 | 82.6% | 88.9% | (many diffs) |
29 | mouse | 12298 | Cacnb4 | calcium channel, voltage-de... | NM_001285427.1 | 81.6% | 89.8% | (many diffs) |
30 | mouse | 12298 | Cacnb4 | calcium channel, voltage-de... | XM_006497641.3 | 81.6% | 89.8% | (many diffs) |
31 | mouse | 12298 | Cacnb4 | calcium channel, voltage-de... | XM_011239015.1 | 81.6% | 89.8% | (many diffs) |
32 | mouse | 12298 | Cacnb4 | calcium channel, voltage-de... | XM_017315163.1 | 81.6% | 89.8% | (many diffs) |
33 | mouse | 12298 | Cacnb4 | calcium channel, voltage-de... | XM_006497642.3 | 63.6% | 67.3% | (many diffs) |
34 | mouse | 12298 | Cacnb4 | calcium channel, voltage-de... | XM_017315165.1 | 63.6% | 67.3% | (many diffs) |
35 | mouse | 12298 | Cacnb4 | calcium channel, voltage-de... | NM_001285428.1 | 58.1% | 63.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1629
- ORF length:
- 1560
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcctcctcc tcctacgcca agaacgggac cgcggacggg ccgcactccc 121 ccacctcgca ggtggcccga ggcaccacaa cccggaggag caggttgaaa agatccgatg 181 gcagcaccac ttcgaccagc ttcatcctca gacagggttc agcggattcc tacacaagca 241 ggccgtctga ctccgatgtc tctttggaag aggaccggga agcaattcga caggagagag 301 aacagcaagc agctatccag cttgagagag caaagtccaa acctgtagca tttgccgtga 361 agacaaatgt gagctactgc ggcgccctgg acgaggatgt gcctgttcca agcacagcta 421 tctcctttga tgctaaagac tttctacata ttaaagagaa atataacaat gattggtgga 481 taggaaggct ggtgaaagag ggctgtgaaa ttggcttcat tccaagtcca ctcagattgg 541 agaacatacg gatccagcaa gaacaaaaaa gaggacgttt tcacggaggg aaatcaagtg 601 gaaattcttc ttcaagtctt ggagaaatgg tatctgggac attccgagca actcccacat 661 caacagcaaa acagaagcaa aaagtgacgg agcacattcc tccttacgat gttgtaccgt 721 caatgcgtcc ggtggtgtta gtggggccgt cactgaaagg ttacgaggta acagacatga 781 tgcagaaagc cctctttgat ttcctgaagc acaggtttga tgggaggatt tcaataacga 841 gagtgacagc tgacatttct cttgctaaga ggtctgtcct aaataatccc agcaagagag 901 caataattga acgttcgaac acccggtcca gcttagcgga agtacaaagt gaaattgaaa 961 gaatctttga gttggcaaga tctttgcaac tggttgttct tgatgcagac accatcaatc 1021 acccagcaca acttataaag acttccttag caccaattat tgttcatgta aaagtctcat 1081 ctccaaaggt tttacagcgg ttgattaaat ctagaggaaa gtcacaaagt aaacacttga 1141 atgttcaact ggtggcagct gataaacttg cacaatgccc cccagaaatg tttgatgtta 1201 tattggatga aaatcagctt gaggatgcat gtgaacatct aggggagtac ctggaggcgt 1261 actggcgtgc cacccacaca accagtagca cacccatgac cccgctgctg ggaaggaatt 1321 tgggctccac ggcactctca ccatatccca cagcaatttc tgggttacag agtcagcgaa 1381 tgaggcacag caaccactcc acagagaact ctccaattga aagacgaagt ctaatgacct 1441 ctgatgaaaa ttatcacaat gaaagggctc ggaagagtag gaaccgcttg tcttccagtt 1501 ctcagcatag ccgagatcat taccctcttg tggaagaaga ttaccctgac tcataccagg 1561 acacttacaa accccatagg aaccgaggat cacctggggg atatagccat gactcccgac 1621 ataggctttt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1681 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1741 tcaacctctg gatta