Construct: ORF ccsbBroad304_01045
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006852.1_s304c1, BRDN0000402517
- Derived from:
- ccsbBroadEn_01045
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT2A (4502)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01045
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4502 | MT2A | metallothionein 2A | NM_005953.5 | 100% | 100% | |
| 2 | human | 4495 | MT1G | metallothionein 1G | NM_005950.3 | 95% | 95% | (many diffs) |
| 3 | human | 4495 | MT1G | metallothionein 1G | NM_001301267.2 | 93.5% | 93.5% | (many diffs) |
| 4 | human | 4501 | MT1X | metallothionein 1X | NM_005952.4 | 92.9% | 90.1% | (many diffs) |
| 5 | human | 4493 | MT1E | metallothionein 1E | NM_175617.4 | 92.3% | 93.4% | (many diffs) |
| 6 | human | 4489 | MT1A | metallothionein 1A | NM_005946.3 | 92.3% | 90.1% | (many diffs) |
| 7 | human | 4496 | MT1H | metallothionein 1H | NM_005951.2 | 91.8% | 90.1% | (many diffs) |
| 8 | human | 4494 | MT1F | metallothionein 1F | NM_005949.4 | 91.2% | 91.8% | (many diffs) |
| 9 | human | 645745 | MT1HL1 | metallothionein 1H like 1 | NM_001276687.2 | 91.2% | 88.5% | (many diffs) |
| 10 | human | 4499 | MT1M | metallothionein 1M | NM_176870.3 | 89.6% | 80.3% | (many diffs) |
| 11 | human | 4490 | MT1B | metallothionein 1B | NM_005947.3 | 88.5% | 85.2% | (many diffs) |
| 12 | human | 4494 | MT1F | metallothionein 1F | NM_001301272.1 | 56.7% | 53.9% | (many diffs) |
| 13 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_027781.1 | 43% | (many diffs) | |
| 14 | human | 4498 | MT1JP | metallothionein 1J, pseudogene | NR_036677.1 | 40.6% | (many diffs) | |
| 15 | human | 4500 | MT1L | metallothionein 1L, pseudogene | NR_001447.2 | 37.6% | (many diffs) | |
| 16 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_003669.2 | 36.4% | (many diffs) | |
| 17 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_003658.2 | 30.9% | (many diffs) | |
| 18 | mouse | 17750 | Mt2 | metallothionein 2 | NM_008630.2 | 88.5% | 86.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 249
- ORF length:
- 183
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tcccaactgc tcctgcgccg ccggtgactc ctgcacctgc gccggctcct 121 gcaaatgcaa agagtgcaaa tgcacctcct gcaagaaaag ctgctgctcc tgctgccctg 181 tgggctgtgc caagtgtgcc cagggctgca tctgcaaagg ggcgtcggac aagtgcagct 241 gctgcgcctg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 301 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 361 tcaacctctg gatta