Construct: ORF ccsbBroad304_01278

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008264.1_s304c1, BRDN0000399731
Derived from:
ccsbBroadEn_01278
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKACB (5567)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01278
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5567 PRKACB protein kinase cAMP-activat... NM_207578.3 100% 100%
2 human 5567 PRKACB protein kinase cAMP-activat... XM_017001713.2 99.6% 99.6% 764_766delAGC
3 human 5567 PRKACB protein kinase cAMP-activat... XM_017001717.1 95.6% 95.7% (many diffs)
4 human 5567 PRKACB protein kinase cAMP-activat... NM_001300917.2 94.6% 93.7% (many diffs)
5 human 5567 PRKACB protein kinase cAMP-activat... XM_017001716.1 94.6% 96.1% (many diffs)
6 human 5567 PRKACB protein kinase cAMP-activat... XM_017001720.1 94.5% 94.5% 0_1ins40;2delT;6T>C
7 human 5567 PRKACB protein kinase cAMP-activat... XM_017001715.1 94.5% 94.5% (many diffs)
8 human 5567 PRKACB protein kinase cAMP-activat... XM_017001718.2 94.3% 93.4% (many diffs)
9 human 5567 PRKACB protein kinase cAMP-activat... XM_017001711.1 94.2% 94.2% (many diffs)
10 human 5567 PRKACB protein kinase cAMP-activat... XM_017001719.1 94.1% 94.1% (many diffs)
11 human 5567 PRKACB protein kinase cAMP-activat... XM_017001714.1 94.1% 94.2% (many diffs)
12 human 5567 PRKACB protein kinase cAMP-activat... XM_017001712.2 93.9% 94.6% (many diffs)
13 human 5567 PRKACB protein kinase cAMP-activat... XM_017001709.1 93.8% 93.9% (many diffs)
14 human 5567 PRKACB protein kinase cAMP-activat... XM_017001710.2 93.6% 94.2% (many diffs)
15 human 5567 PRKACB protein kinase cAMP-activat... XM_017001707.1 93.1% 93.1% (many diffs)
16 human 5567 PRKACB protein kinase cAMP-activat... XM_017001708.2 92.9% 93.5% (many diffs)
17 human 5567 PRKACB protein kinase cAMP-activat... XM_017001706.1 92.8% 92.8% (many diffs)
18 human 5567 PRKACB protein kinase cAMP-activat... NM_001300915.2 92.5% 93.1% (many diffs)
19 human 5567 PRKACB protein kinase cAMP-activat... NM_001300916.2 82.4% 80.9% (many diffs)
20 human 5567 PRKACB protein kinase cAMP-activat... XM_005271020.2 82.2% 80.6% (many diffs)
21 human 5567 PRKACB protein kinase cAMP-activat... NM_002731.3 73% 72.9% 766_781delinsA;786_1053delinsT
22 human 5567 PRKACB protein kinase cAMP-activat... XM_005271018.4 69.9% 69.8% (many diffs)
23 human 5567 PRKACB protein kinase cAMP-activat... XM_005271017.3 69.4% 70% (many diffs)
24 human 5567 PRKACB protein kinase cAMP-activat... XM_005271016.1 69.3% 69.1% (many diffs)
25 human 5567 PRKACB protein kinase cAMP-activat... NM_001242859.2 69.3% 69.1% (many diffs)
26 human 5567 PRKACB protein kinase cAMP-activat... XM_006710758.2 69.1% 69.2% (many diffs)
27 human 5567 PRKACB protein kinase cAMP-activat... NM_001242858.2 69.1% 68.3% (many diffs)
28 human 5567 PRKACB protein kinase cAMP-activat... XM_005271019.1 69% 68.9% (many diffs)
29 human 5567 PRKACB protein kinase cAMP-activat... XM_005271015.3 68.8% 69.5% (many diffs)
30 human 5567 PRKACB protein kinase cAMP-activat... NM_001242857.2 68.7% 68.4% (many diffs)
31 human 5567 PRKACB protein kinase cAMP-activat... NM_001242860.2 68.6% 68.6% (many diffs)
32 human 5567 PRKACB protein kinase cAMP-activat... NM_001242862.2 66.4% 63.2% (many diffs)
33 human 5567 PRKACB protein kinase cAMP-activat... NM_182948.4 62.8% 61.5% (many diffs)
34 human 5567 PRKACB protein kinase cAMP-activat... NM_001242861.2 59.9% 60% (many diffs)
35 human 5566 PRKACA protein kinase cAMP-activat... NM_002730.4 55.6% 68% (many diffs)
36 human 5566 PRKACA protein kinase cAMP-activat... NM_207518.3 53.4% 64.6% (many diffs)
37 mouse 18749 Prkacb protein kinase, cAMP depend... NM_011100.4 63.5% 70.6% (many diffs)
38 mouse 18749 Prkacb protein kinase, cAMP depend... NM_001164198.1 59.8% 66.9% (many diffs)
39 mouse 18749 Prkacb protein kinase, cAMP depend... NM_001164199.1 59.8% 66.6% (many diffs)
40 mouse 18749 Prkacb protein kinase, cAMP depend... NM_001164200.1 55.2% 59.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
837
ORF length:
771
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg gaacgcggcg accgccaaga aaggcagcga ggtggagagc gtgaaagagt
121ttctagccaa agccaaagaa gactttttga aaaaatggga GAATCCAACT CAGAATAATG
181CCGGACTTGA AGATTTTGAA AGGAAAAAAA CCCTTGGAAC AGGTTCATTT GGAAGAGTCA
241TGTTGGTAAA ACACAAAGCC ACTGAACAGT ATTATGCCAT GAAGATCTTA GATAAGCAGA
301AGGTTGTTAA ACTGAAGCAA ATAGAGCATA CTTTGAATGA GAAAAGAATA TTACAGGCAG
361TGAATTTTCC TTTCCTTGTT CGACTGGAGT ATGCTTTTAA GGATAATTCT AATTTATACA
421TGGTTATGGA ATATGTCCCT GGGGGTGAAA TGTTTTCACA TCTAAGAAGA ATTGGAAGGT
481TCAGTGAGCC CCATGCACGG TTCTATGCAG CTCAGATAGT GCTAACATTC GAGTACCTCC
541ATTCACTAGA CCTCATCTAC AGAGATCTAA AACCTGAAAA TCTCTTAATT GACCATCAAG
601GCTATATCCA GGTCACAGAC TTTGGGTTTG CCAAAAGAGT TAAAGGCAGA ACTTGGACAT
661TATGTGGAAC TCCAGAGTAT TTGGCTCCAG AAATAATTCT CAGCAAGGGC TACAATAAGG
721CAGTGGATTG GTGGGCATTA GGAGTGCTAA TCTATGAAAT GGCAGCTGGC TATCCCCCAT
781TCTTTGCAGA CCAACCAATT CAGATTTATG AAAAGATTGT TTCTGGAAAG AACTTTTGCC
841CAACTTTCTT GTACaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
901ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
961tta

Download FASTA (ORF) (Full)