Construct: ORF ccsbBroad304_01279

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001545.1_s304c1, BRDN0000409248
Derived from:
ccsbBroadEn_01279
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKAG1 (5571)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01279
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5571 PRKAG1 protein kinase AMP-activate... NM_002733.5 100% 100%
2 human 5571 PRKAG1 protein kinase AMP-activate... NM_001206709.2 97.3% 97.3% 250_276del
3 human 5571 PRKAG1 protein kinase AMP-activate... NM_001206710.2 90.3% 90.3% 0_1ins96
4 human 5571 PRKAG1 protein kinase AMP-activate... XM_011538562.2 90.3% 90.3% 0_1ins96
5 human 5571 PRKAG1 protein kinase AMP-activate... XM_005269020.2 74.6% 74.6% 0_1ins252
6 human 5571 PRKAG1 protein kinase AMP-activate... XM_017019622.1 74.6% 74.6% 0_1ins252
7 human 5571 PRKAG1 protein kinase AMP-activate... XM_017019623.1 74.6% 74.6% 0_1ins252
8 human 5571 PRKAG1 protein kinase AMP-activate... XM_005269019.4 72.7% 76.6% 1_221del;277_278ins110
9 mouse 19082 Prkag1 protein kinase, AMP-activat... NM_016781.2 87.5% 97.2% (many diffs)
10 mouse 19082 Prkag1 protein kinase, AMP-activat... XM_011245520.1 79.6% 89.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1059
ORF length:
993
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gacggtcatt tcttcagata gctccccagc tgtggaaaat gagcatcctc
121aagagacccc agaatccaac aatagcgtgt atacttcctt catgaagtct catcgctgct
181atgacctgat tcccacaagc tccaaattgg ttgtatttga tacgtccctg caggtgaaga
241aagctttttt tgctttggtg actaacggtg tacgagctgc ccctttatgg gatagtaaga
301agcaaagttt tgtgggcatg ctgaccatca ctgatttcat caatatcctg caccgctact
361ataaatcagc cttggtacag atctatgagc tagaagaaca caagatagaa acttggagag
421aggtgtatct ccaggactcc tttaaaccgc ttgtctgcat ttctcctaat gccagcttgt
481ttgatgctgt ctcttcatta attcggaaca agatccacag gctgccagtt attgacccag
541aatcaggcaa tactttgtac atcctcaccc acaagcgcat tctgaagttc ctcaaattgt
601ttatcactga gttccccaag ccagagttca tgtccaagtc tctggaagag ctacagattg
661gcacctatgc caatattgct atggttcgca ctaccacccc cgtctatgtg gctctgggga
721tttttgtaca gcatcgagtc tcagccctgc cagtggtgga tgagaagggg cgtgtggtgg
781acatctactc caagtttgat gttatcaatc tggcagcaga aaagacctac aacaacctag
841atgtatctgt gactaaagcc ttgcaacatc gatcacatta ctttgagggt gttctcaagt
901gctacctgca tgagactctg gagaccatca tcaacaggct agtggaagca gaggttcacc
961gacttgtagt ggtggatgaa aatgatgtgg tcaagggaat tgtatcactg tctgacatcc
1021tgcaggccct ggtgctcaca ggtggagaga agaagccctg cccaactttc ttgtacaaag
1081tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1141gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)