Construct: ORF ccsbBroad304_01486
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011233.1_s304c1, BRDN0000408328
- Derived from:
- ccsbBroadEn_01486
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATXN1 (6310)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01486
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6310 | ATXN1 | ataxin 1 | NM_000332.3 | 100% | 100% | |
| 2 | human | 6310 | ATXN1 | ataxin 1 | NM_001128164.1 | 100% | 100% | |
| 3 | human | 6310 | ATXN1 | ataxin 1 | NM_001357857.2 | 22.6% | 6.9% | 0_1ins29;555_556ins1861 |
| 4 | mouse | 20238 | Atxn1 | ataxin 1 | NM_001199304.1 | 83.5% | 86.8% | (many diffs) |
| 5 | mouse | 20238 | Atxn1 | ataxin 1 | NM_009124.6 | 83.5% | 86.8% | (many diffs) |
| 6 | mouse | 20238 | Atxn1 | ataxin 1 | XM_011244381.2 | 83.5% | 86.8% | (many diffs) |
| 7 | mouse | 20238 | Atxn1 | ataxin 1 | XM_011244383.2 | 83.5% | 86.8% | (many diffs) |
| 8 | mouse | 20238 | Atxn1 | ataxin 1 | XM_011244385.2 | 83.5% | 86.8% | (many diffs) |
| 9 | mouse | 20238 | Atxn1 | ataxin 1 | XM_011244386.2 | 83.5% | 86.8% | (many diffs) |
| 10 | mouse | 20238 | Atxn1 | ataxin 1 | XM_011244387.2 | 83.5% | 86.8% | (many diffs) |
| 11 | mouse | 20238 | Atxn1 | ataxin 1 | XM_011244388.1 | 83.5% | 86.8% | (many diffs) |
| 12 | mouse | 20238 | Atxn1 | ataxin 1 | XM_011244389.2 | 83.5% | 86.8% | (many diffs) |
| 13 | mouse | 20238 | Atxn1 | ataxin 1 | XM_011244390.2 | 83.5% | 86.8% | (many diffs) |
| 14 | mouse | 20238 | Atxn1 | ataxin 1 | XM_011244391.2 | 83.5% | 86.8% | (many diffs) |
| 15 | mouse | 20238 | Atxn1 | ataxin 1 | XM_011244392.1 | 83.5% | 86.8% | (many diffs) |
| 16 | mouse | 20238 | Atxn1 | ataxin 1 | XM_011244393.1 | 83.5% | 86.8% | (many diffs) |
| 17 | mouse | 20238 | Atxn1 | ataxin 1 | XM_017315462.1 | 83.5% | 86.8% | (many diffs) |
| 18 | mouse | 20238 | Atxn1 | ataxin 1 | XM_017315463.1 | 83.5% | 86.8% | (many diffs) |
| 19 | mouse | 20238 | Atxn1 | ataxin 1 | NM_001199305.1 | 82.7% | 86% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 68
- ORF end:
- 2513
- ORF length:
- 2445
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcacatg aaatccaacc aagagcggag caacgaatgc ctgcctccca agaagcgcga 121 gatccccgcc accagccggt cctccgagga gaaggcccct accctgccca gcgacaacca 181 ccgggtggag ggcacagcat ggctcccggg caaccctggt ggccggggcc acgggggcgg 241 gaggcatggg ccggcaggga cctcggtgga gcttggttta caacagggaa taggtttaca 301 caaagcattg tccacagggc tggactactc cccgcccagc gctcccaggt ctgtccccgt 361 ggccaccacg ctgcctgccg cgtacgccac cccgcagcca gggaccccgg tgtcccccgt 421 gcagtacgct cacctgccgc acaccttcca gttcattggg tcctcccaat acagtggaac 481 ctatgccagc ttcatcccat cacagctgat ccccccaacc gccaaccccg tcaccagtgc 541 agtggcctcg gccgcagggg ccaccactcc atcccagcgc tcccagctgg aggcctattc 601 cactctgctg gccaacatgg gcagtctgag ccagacgccg ggacacaagg ctgagcagca 661 gcagcagcag cagcagcagc agcagcagca gcatcagcat cagcagcagc agcagcagca 721 gcagcagcag cagcagcagc agcacctcag cagggctccg gggctcatca ccccggggtc 781 ccccccacca gcccagcaga accagtacgt ccacatttcc agttctccgc agaacaccgg 841 ccgcaccgcc tctcctccgg ccatccccgt ccacctccac ccccaccaga cgatgatccc 901 acacacgctc accctggggc ccccctccca ggtcgtcatg caatacgccg actccggcag 961 ccactttgtc cctcgggagg ccaccaagaa agctgagagc agccggctgc agcaggccat 1021 ccaggccaag gaggtcctga acggtgagat ggagaagagc cggcggtacg gggccccgtc 1081 ctcagccgac ctgggcctgg gcaaggcagg cggcaagtcg gttcctcacc cgtacgagtc 1141 caggcacgtg gtggtccacc cgagcccctc agactacagc agtcgtgatc cttcgggggt 1201 ccgggcctct gtgatggtcc tgcccaacag caacacgccc gcagctgacc tggaggtgca 1261 acaggccact catcgtgaag cctccccttc taccctcaac gacaaaagtg gcctgcattt 1321 agggaagcct ggccaccggt cctacgcgct ctcaccccac acggtcattc agaccacaca 1381 cagtgcttca gagccactcc cggtgggact gccagccacg gccttctacg cagggactca 1441 accccctgtc atcggctacc tgagcggcca gcagcaagca atcacctacg ccggcagcct 1501 gccccagcac ctggtgatcc ccggcacaca gcccctgctc atcccggtcg gcagcactga 1561 catggaagcg tcgggggcag ccccggccat agtcacgtca tccccccagt ttgctgcagt 1621 gcctcacacg ttcgtcacca ccgcccttcc caagagcgag aacttcaacc ctgaggccct 1681 ggtcacccag gccgcctacc cagccatggt gcaggcccag atccacctgc ctgtggtgca 1741 gtccgtggcc tccccggcgg cggctccccc tacgctgcct ccctacttca tgaaaggctc 1801 catcatccag ttggccaacg gggagctaaa gaaggtggaa gacttaaaaa cagaagattt 1861 catccagagt gcagagataa gcaacgacct gaagatcgac tccagcaccg tagagaggat 1921 tgaagacagc catagcccgg gcgtggccgt gatacagttc gccgtcgggg agcaccgagc 1981 ccaggtcagc gttgaagttt tggtagagta tccttttttt gtgtttggac agggctggtc 2041 atcctgctgt ccggagagaa ccagccagct ctttgatttg ccgtgttcca aactctcagt 2101 tggggatgtc tgcatctcgc ttaccctcaa gaacctgaag aacggctctg ttaaaaaggg 2161 ccagcccgtg gatcccgcca gcgtcctgct gaagcactca aaggccgacg gcctggcggg 2221 cagcagacac aggtatgccg agcaggaaaa cggaatcaac caggggagtg cccagatgct 2281 ctctgagaat ggcgaactga agtttccaga gaaaatggga ttgcctgcag cgcccttcct 2341 caccaaaata gaacccagca agcccgcggc aacgaggaag aggaggtggt cggcgccaga 2401 gagccgcaaa ctggagaagt cagaagacga accacctttg actcttccta agccttctct 2461 aattcctcag gaggttaaga tttgcattga aggccggtct aatgtaggca agttgccaac 2521 tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac 2581 gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggatta