Construct: ORF ccsbBroad304_01576

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008133.1_s304c1, BRDN0000400294
Derived from:
ccsbBroadEn_01576
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SOAT1 (6646)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01576
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6646 SOAT1 sterol O-acyltransferase 1 NM_003101.6 100% 100%
2 human 6646 SOAT1 sterol O-acyltransferase 1 XM_011509911.1 100% 100%
3 human 6646 SOAT1 sterol O-acyltransferase 1 NM_001252511.1 89.3% 89.2% 0_1ins175;2delT
4 human 6646 SOAT1 sterol O-acyltransferase 1 NM_001252512.1 88.1% 88.1% 0_1ins195
5 human 6646 SOAT1 sterol O-acyltransferase 1 XM_011509912.1 60.1% 60.1% 990_990delGinsCTTTGGT;993_994ins651
6 human 6646 SOAT1 sterol O-acyltransferase 1 NR_045530.2 22.6% (many diffs)
7 mouse 20652 Soat1 sterol O-acyltransferase 1 NM_009230.3 82.2% 85.4% (many diffs)
8 mouse 20652 Soat1 sterol O-acyltransferase 1 XM_006496719.3 77.7% 81.4% (many diffs)
9 mouse 20652 Soat1 sterol O-acyltransferase 1 XM_011238778.2 77.7% 81.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1716
ORF length:
1650
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt gggtgaagag aagatgtctc taagaaaccg gctgtcaaag tccagggaaa
121atcctgagga agatgaagac cagagaaacc ctgcaaagga gtccctagag acacctagta
181atggtcgaat tgacataaaa cagttgatag caaagaagat aaagttgaca gcagaggcag
241aggaattgaa gccatttttt atgaaggaag ttggcagtca ctttgatgat tttgtgacca
301atctcattga aaagtcagca tcattagata atggtgggtg cgctctcaca accttttctg
361ttcttgaagg agagaaaaac aaccatagag cgaaggattt gagagcacct ccagaacaag
421gaaagatttt tattgcaagg cgctctctct tagatgaact gcttgaagtg gaccacatca
481gaacaatata tcacatgttt attgccctcc tcattctctt tatcctcagc acacttgtag
541tagattacat tgatgaagga aggctggtgc ttgagttcag cctcctgtct tatgcttttg
601gcaaatttcc taccgttgtt tggacctggt ggatcatgtt cctgtctaca ttttcagttc
661cctattttct gtttcaacat tgggccactg gctatagcaa gagttctcat ccgctgatcc
721gttctctctt ccatggcttt cttttcatga tcttccagat tggagttcta ggttttggac
781caacatatgt tgtgttagca tatacactgc caccagcttc ccggttcatc attatattcg
841agcagattcg ttttgtaatg aaggcccact catttgtcag agagaacgtg cctcgggtac
901taaattcagc taaggagaaa tcaagcactg ttccaatacc tacagtcaac cagtatttgt
961acttcttatt tgctcctacc cttatctacc gtgacagcta tcccaggaat cccactgtaa
1021gatggggtta tgtcgctatg aagtttgcac aggtctttgg ttgctttttc tatgtgtact
1081acatctttga aaggctttgt gcccccttgt ttcggaatat caaacaggag cccttcagcg
1141ctcgtgttct ggtcctatgt gtatttaact ccatcttgcc aggtgtgctg attctcttcc
1201ttactttttt tgcctttttg cactgctggc tcaatgcctt tgctgagatg ttacgctttg
1261gtgacaggat gttctataag gattggtgga actccacgtc atactccaac tattatagaa
1321cctggaatgt ggtggtccat gactggctat attactatgc ttacaaggac tttctctggt
1381ttttctccaa gagattcaaa tctgctgcca tgttagctgt ctttgctgta tctgctgtag
1441tacacgaata tgccttggct gtttgcttga gctttttcta tcccgtgctc ttcgtgctct
1501tcatgttctt tggaatggct ttcaacttca ttgtcaatga tagtcggaaa aagccgattt
1561ggaatgttct gatgtggact tctcttttct tgggcaatgg agtcttactc tgcttttatt
1621ctcaagaatg gtatgcacgt cagcactgtc ctctgaaaaa tcccacattt ttggattatg
1681tccggccacg ttcctggact tgtcgttacg tgttttaccc aactttcttg tacaaagtgg
1741ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1801ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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