Construct: ORF ccsbBroad304_02903
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011478.1_s304c1, BRDN0000461530
- Derived from:
- ccsbBroadEn_02903
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSMF (26012)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02903
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | NM_015537.4 | 100% | 100% | |
| 2 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | NM_001130969.1 | 99.6% | 99.6% | 704_709delCCATCT |
| 3 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | NM_001130970.1 | 95.8% | 95.6% | 704_705ins53;706A>G;710_710delCinsGTAATCCAGAG |
| 4 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | NM_001130971.1 | 95.6% | 95.6% | 704_705ins69 |
| 5 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | XM_005266061.5 | 94.3% | 94.3% | 825_826ins90 |
| 6 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | NM_001178064.1 | 93.9% | 93.9% | 704_709delCCATCT;831_832ins90 |
| 7 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | XM_011518496.3 | 90.2% | 89.9% | (many diffs) |
| 8 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | XM_005266062.5 | 89.9% | 89.9% | 704_705ins69;756_757ins90 |
| 9 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | XM_017014597.2 | 59.4% | 47.7% | (many diffs) |
| 10 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | XM_011518497.2 | 49.8% | 49.8% | 0_1ins795 |
| 11 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | NM_001039387.1 | 89.2% | 93.9% | (many diffs) |
| 12 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | NM_001039386.1 | 88.9% | 93.6% | (many diffs) |
| 13 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | NM_001177654.1 | 88.7% | 93.3% | (many diffs) |
| 14 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | NM_020276.3 | 85.3% | 89.6% | (many diffs) |
| 15 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_006498200.1 | 85.1% | 89.6% | (many diffs) |
| 16 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | NM_001177655.1 | 83.8% | 88.3% | (many diffs) |
| 17 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_006498201.1 | 83.5% | 87.9% | (many diffs) |
| 18 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319141.1 | 80.5% | 84.6% | (many diffs) |
| 19 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319140.1 | 80.2% | 84.4% | (many diffs) |
| 20 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_006498202.3 | 79.9% | 83.9% | (many diffs) |
| 21 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_006498203.3 | 79.6% | 83.9% | (many diffs) |
| 22 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319142.1 | 76.9% | 80.7% | (many diffs) |
| 23 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319143.1 | 76.7% | 80.7% | (many diffs) |
| 24 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319144.1 | 57% | 60.3% | (many diffs) |
| 25 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319145.1 | 53.6% | 56.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1653
- ORF length:
- 1584
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcgccgcc gcctccagga ggagggcgct gaggagcgag gccatgtcct 121 cggtggcggc caaagtgcga gcagcccgag cgtttggaga gtacctgtcc cagagtcacc 181 ctgagaaccg caacggcgca gatcacctgc tggctgatgc ctactctggc cacgacgggt 241 cccccgagat gcagccggcc ccccagaaca agcgccgcct gtccctcgtc tccaacggct 301 gctacgaggg cagcctctca gaggagccca gcattaggaa gcccgcaggc gagggccctc 361 agcctcgagt gtacaccatc tctggggagc ctgccctgct gcccagccct gaggcggagg 421 ccattgagct ggcggtggtg aaggggcggc ggcagcggca ccctcaccat cacagccagc 481 ccctgcgcgc cagccctggt ggcagccggg aggacgtcag caggccctgc cagagctggg 541 cgggcagccg ccagggctcc aaggagtgcc ccggatgtgc ccagctggct cctggcccca 601 cccctcgggc ctttgggctg gaccagccac ctctgcctga gacctccggt cgccgcaaga 661 agctggagag gatgtacagc gttgaccgtg tgtctgacga catccctatt cgtacctggt 721 tccccaagga aaatcttttc agcttccaga cagcaaccac aactatgcaa gcggtgttca 781 ggggctacgc ggagaggaag cgccggaaac gggagaatga ttccgcgtct gtaatccaga 841 ggaacttccg caaacacctg cgcatggtcg gcagccggag ggtgaaggcc cagacgttcg 901 ctgagcggcg cgagcggagc ttcagccggt cctggagcga ccccaccccc atgaaagccg 961 acacttccca cgactcccga gacagcagtg acctgcagag ctcccactgc acgctggacg 1021 aggccttcga ggacctggac tgggacactg agaagggcct ggaggctgtg gcctgcgaca 1081 ccgaaggctt cgtgccacca aaggtcatgc tcatttcctc caaggtgccc aaggctgagt 1141 acatccccac tatcatccgc cgggatgacc cctccatcat ccccatcctc tacgaccatg 1201 agcacgcaac cttcgaggac atccttgagg agatagagag gaagctgaac gtctaccaca 1261 agggagccaa gatctggaaa atgctgattt tctgccaggg aggtcctgga cacctctatc 1321 tcctcaagaa caaggtggcc acctttgcca aagtggagaa ggaagaggac atgattcact 1381 tctggaagcg gctgagccgc ctgatgagca aagtgaaccc agagccgaac gtcatccaca 1441 tcatgggctg ctacattctg gggaacccca atggagagaa gctgttccag aacctcagga 1501 ccctcatgac tccttatagg gtcaccttcg agtcacccct ggagctctca gcccaaggga 1561 agcagatgat cgagacgtac tttgacttcc ggttgtatcg cctgtggaag agccgccagc 1621 actcgaagct gctggacttt gacgacgtcc tgttgccaac tttcttgtac aaagtggttg 1681 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1741 accggtggcg cgttaagtcg acaatcaacc tctggatta