Construct: ORF ccsbBroad304_03523

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015088.1_s304c1, BRDN0000461634
Derived from:
ccsbBroadEn_03523
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RALGPS2 (55103)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03523
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55103 RALGPS2 Ral GEF with PH domain and ... NM_152663.5 100% 100%
2 human 55103 RALGPS2 Ral GEF with PH domain and ... XM_006711410.3 100% 100%
3 human 55103 RALGPS2 Ral GEF with PH domain and ... NM_001286247.1 95.5% 95.5% 1245_1246ins78
4 human 55103 RALGPS2 Ral GEF with PH domain and ... XM_011509688.1 94.5% 93.5% 1628_1629ins92;1653_1654insTGAG
5 human 55103 RALGPS2 Ral GEF with PH domain and ... XM_006711411.3 65.2% 65.3% 0_1ins605;2_6delTGGGC
6 human 55103 RALGPS2 Ral GEF with PH domain and ... XM_017001591.2 60.7% 60.8% 0_1ins605;2_6delTGGGC;645_646ins78
7 mouse 78255 Ralgps2 Ral GEF with PH domain and ... NM_001159965.1 88% 96.4% (many diffs)
8 mouse 78255 Ralgps2 Ral GEF with PH domain and ... NM_001159966.1 88% 96.4% (many diffs)
9 mouse 78255 Ralgps2 Ral GEF with PH domain and ... NM_023884.4 88% 96.4% (many diffs)
10 mouse 78255 Ralgps2 Ral GEF with PH domain and ... XM_006497028.3 88% 96.4% (many diffs)
11 mouse 78255 Ralgps2 Ral GEF with PH domain and ... XM_006497029.2 88% 96.4% (many diffs)
12 mouse 78255 Ralgps2 Ral GEF with PH domain and ... XM_006497030.2 88% 96.4% (many diffs)
13 mouse 78255 Ralgps2 Ral GEF with PH domain and ... XM_006497031.3 88% 96.4% (many diffs)
14 mouse 78255 Ralgps2 Ral GEF with PH domain and ... XM_006497032.3 88% 96.4% (many diffs)
15 mouse 78255 Ralgps2 Ral GEF with PH domain and ... NM_001159967.1 83.9% 92.3% (many diffs)
16 mouse 78255 Ralgps2 Ral GEF with PH domain and ... NM_001159968.1 82.7% 90.6% (many diffs)
17 mouse 78255 Ralgps2 Ral GEF with PH domain and ... XM_006497033.2 66.3% 73.2% (many diffs)
18 mouse 78255 Ralgps2 Ral GEF with PH domain and ... XM_017312931.1 62.1% 69.1% (many diffs)
19 mouse 78255 Ralgps2 Ral GEF with PH domain and ... XR_373625.2 21.1% (many diffs)
20 mouse 78255 Ralgps2 Ral GEF with PH domain and ... NR_027640.1 20.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1815
ORF length:
1749
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga cctaatgaac gggcaggcaa gcagtgtcaa tattgcagct actgcttctg
121agaaaagtag cagctctgaa tccttaagtg acaaaggctc tgaattgaag aaaagctttg
181atgctgtggt attcgatgtt cttaaggtta caccagaaga atatgcgggt cagataacat
241taatggatgt tccagtattt aaagctattc aaccagatga gctttcaagt tgtggatgga
301ataaaaaaga aaaatatagt tctgcaccaa atgcagttgc cttcacaaga agattcaatc
361atgtaagctt ttgggttgtt agagagattc ttcatgctca aacattaaaa attagagcag
421aagttttgag ccactatatt aaaactgcta agaaactgta tgagctgaat aaccttcatg
481cacttatggc agtggtttct ggcctacaga gtgccccaat tttcaggttg actaaaacat
541gggcgttatt aagtcgaaaa gacaaaacta cctttgaaaa attagaatat gtaatgagta
601aagaagataa ctacaaaaga ctcagagact atataagtag cttaaagatg acaccttgca
661ttccctattt aggtatctat ttgtcagatt taacatacat cgattcagca tacccatcaa
721ctggcagcat tctagaaaat gagcaaagat caaatttaat gaataatatc cttcgaataa
781tttctgattt acagcagtct tgtgaatatg atattcccat gttgcctcat gtccaaaaat
841atctcaactc tgttcagtat atagaagaac tacaaaaatt tgtggaagac gataattaca
901agctttcatt aaagatagaa ccagggacaa gcaccccacg ttctgctgct tccagagaag
961atttagtagg tcctgaagta ggagcgtctc cacagagtgg acgaaaaagt gtggcagctg
1021aaggagcctt gctcccacag acaccgccat cccctcggaa tctgattcca catggacata
1081ggaagtgcca tagtttgggt tataatttca ttcataaaat gaacacagca gaatttaaga
1141gtgcaacgtt tccaaatgca ggaccaagac atctgttaga tgatagcgtc atggagcccc
1201atgcgccatc tcgaggccaa gctgaaagtt ctactctttc tagtggaata tcaataggta
1261gcagcgatgg ttctgaacta agtgaagaga cctcatggcc tgcttttgaa aggaacagat
1321tataccattc tctcggcccg gtgacaagag tggcacgaaa tggctatcga agtcacatga
1381aggccagcag ttctgcagaa tcagaagatt tggcagtaca tttatatcca ggagctgtta
1441ctattcaagg tgttctcagg agaaaaactt tgttaaaaga aggcaaaaag cctacagtag
1501catcttggac aaaatattgg gcagctttgt gtgggacaca gcttttttac tatgctgcca
1561aatctctaaa ggctaccgaa agaaaacatt tcaaatcaac atccaataag aacgtatctg
1621tgataggatg gatggtgatg atggctgatg accctgaaca tcctgatctc ttcctgctga
1681ctgactctga gaaaggaaat tcgtacaagt ttcaagctgg caatagaatg aatgcaatgt
1741tatggtttaa gcatttgagt gcagcctgcc aaagtaacaa acaacaggtt cctacaaact
1801tgatgacttt tgagtaccca actttcttgt acaaagtggt tggtaagcct atccctaacc
1861ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1921cgacaatcaa cctctggatt a

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