Construct: ORF ccsbBroad304_03523
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015088.1_s304c1, BRDN0000461634
- Derived from:
- ccsbBroadEn_03523
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RALGPS2 (55103)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03523
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55103 | RALGPS2 | Ral GEF with PH domain and ... | NM_152663.5 | 100% | 100% | |
| 2 | human | 55103 | RALGPS2 | Ral GEF with PH domain and ... | XM_006711410.3 | 100% | 100% | |
| 3 | human | 55103 | RALGPS2 | Ral GEF with PH domain and ... | NM_001286247.1 | 95.5% | 95.5% | 1245_1246ins78 |
| 4 | human | 55103 | RALGPS2 | Ral GEF with PH domain and ... | XM_011509688.1 | 94.5% | 93.5% | 1628_1629ins92;1653_1654insTGAG |
| 5 | human | 55103 | RALGPS2 | Ral GEF with PH domain and ... | XM_006711411.3 | 65.2% | 65.3% | 0_1ins605;2_6delTGGGC |
| 6 | human | 55103 | RALGPS2 | Ral GEF with PH domain and ... | XM_017001591.2 | 60.7% | 60.8% | 0_1ins605;2_6delTGGGC;645_646ins78 |
| 7 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | NM_001159965.1 | 88% | 96.4% | (many diffs) |
| 8 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | NM_001159966.1 | 88% | 96.4% | (many diffs) |
| 9 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | NM_023884.4 | 88% | 96.4% | (many diffs) |
| 10 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | XM_006497028.3 | 88% | 96.4% | (many diffs) |
| 11 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | XM_006497029.2 | 88% | 96.4% | (many diffs) |
| 12 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | XM_006497030.2 | 88% | 96.4% | (many diffs) |
| 13 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | XM_006497031.3 | 88% | 96.4% | (many diffs) |
| 14 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | XM_006497032.3 | 88% | 96.4% | (many diffs) |
| 15 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | NM_001159967.1 | 83.9% | 92.3% | (many diffs) |
| 16 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | NM_001159968.1 | 82.7% | 90.6% | (many diffs) |
| 17 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | XM_006497033.2 | 66.3% | 73.2% | (many diffs) |
| 18 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | XM_017312931.1 | 62.1% | 69.1% | (many diffs) |
| 19 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | XR_373625.2 | 21.1% | (many diffs) | |
| 20 | mouse | 78255 | Ralgps2 | Ral GEF with PH domain and ... | NR_027640.1 | 20.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1815
- ORF length:
- 1749
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cctaatgaac gggcaggcaa gcagtgtcaa tattgcagct actgcttctg 121 agaaaagtag cagctctgaa tccttaagtg acaaaggctc tgaattgaag aaaagctttg 181 atgctgtggt attcgatgtt cttaaggtta caccagaaga atatgcgggt cagataacat 241 taatggatgt tccagtattt aaagctattc aaccagatga gctttcaagt tgtggatgga 301 ataaaaaaga aaaatatagt tctgcaccaa atgcagttgc cttcacaaga agattcaatc 361 atgtaagctt ttgggttgtt agagagattc ttcatgctca aacattaaaa attagagcag 421 aagttttgag ccactatatt aaaactgcta agaaactgta tgagctgaat aaccttcatg 481 cacttatggc agtggtttct ggcctacaga gtgccccaat tttcaggttg actaaaacat 541 gggcgttatt aagtcgaaaa gacaaaacta cctttgaaaa attagaatat gtaatgagta 601 aagaagataa ctacaaaaga ctcagagact atataagtag cttaaagatg acaccttgca 661 ttccctattt aggtatctat ttgtcagatt taacatacat cgattcagca tacccatcaa 721 ctggcagcat tctagaaaat gagcaaagat caaatttaat gaataatatc cttcgaataa 781 tttctgattt acagcagtct tgtgaatatg atattcccat gttgcctcat gtccaaaaat 841 atctcaactc tgttcagtat atagaagaac tacaaaaatt tgtggaagac gataattaca 901 agctttcatt aaagatagaa ccagggacaa gcaccccacg ttctgctgct tccagagaag 961 atttagtagg tcctgaagta ggagcgtctc cacagagtgg acgaaaaagt gtggcagctg 1021 aaggagcctt gctcccacag acaccgccat cccctcggaa tctgattcca catggacata 1081 ggaagtgcca tagtttgggt tataatttca ttcataaaat gaacacagca gaatttaaga 1141 gtgcaacgtt tccaaatgca ggaccaagac atctgttaga tgatagcgtc atggagcccc 1201 atgcgccatc tcgaggccaa gctgaaagtt ctactctttc tagtggaata tcaataggta 1261 gcagcgatgg ttctgaacta agtgaagaga cctcatggcc tgcttttgaa aggaacagat 1321 tataccattc tctcggcccg gtgacaagag tggcacgaaa tggctatcga agtcacatga 1381 aggccagcag ttctgcagaa tcagaagatt tggcagtaca tttatatcca ggagctgtta 1441 ctattcaagg tgttctcagg agaaaaactt tgttaaaaga aggcaaaaag cctacagtag 1501 catcttggac aaaatattgg gcagctttgt gtgggacaca gcttttttac tatgctgcca 1561 aatctctaaa ggctaccgaa agaaaacatt tcaaatcaac atccaataag aacgtatctg 1621 tgataggatg gatggtgatg atggctgatg accctgaaca tcctgatctc ttcctgctga 1681 ctgactctga gaaaggaaat tcgtacaagt ttcaagctgg caatagaatg aatgcaatgt 1741 tatggtttaa gcatttgagt gcagcctgcc aaagtaacaa acaacaggtt cctacaaact 1801 tgatgacttt tgagtaccca actttcttgt acaaagtggt tggtaagcct atccctaacc 1861 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 1921 cgacaatcaa cctctggatt a