Construct: ORF ccsbBroad304_03644

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009183.1_s304c1, BRDN0000403490
Derived from:
ccsbBroadEn_03644
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARFGAP1 (55738)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03644
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55738 ARFGAP1 ADP ribosylation factor GTP... NM_175609.3 100% 100%
2 human 55738 ARFGAP1 ADP ribosylation factor GTP... XM_011528901.2 99.5% 99.5% 863_868delAGGTCC
3 human 55738 ARFGAP1 ADP ribosylation factor GTP... XM_017027950.1 99.5% 99.5% 863_868delAGGTCC
4 human 55738 ARFGAP1 ADP ribosylation factor GTP... XM_006723823.3 97.5% 97.5% 716_717ins30
5 human 55738 ARFGAP1 ADP ribosylation factor GTP... NM_018209.4 97.1% 97.1% 716_717ins30;833_838delAGGTCC
6 human 55738 ARFGAP1 ADP ribosylation factor GTP... NM_001281483.1 86.1% 86.2% 1_1delAins166;3G>C;6_11delAACAAC
7 human 55738 ARFGAP1 ADP ribosylation factor GTP... NM_001281484.1 69.9% 63.4% (many diffs)
8 human 55738 ARFGAP1 ADP ribosylation factor GTP... NM_001281482.2 64.8% 65.1% 716_717ins30;833_1077del;1209_1210ins248
9 human 55738 ARFGAP1 ADP ribosylation factor GTP... XR_001754329.2 40% 1_112del;975_980delAGGTCC;1361_3099del
10 human 55738 ARFGAP1 ADP ribosylation factor GTP... XR_001754330.2 35.6% 1_112del;797_1007del;1566_3485del
11 human 55738 ARFGAP1 ADP ribosylation factor GTP... XR_936575.2 35.5% (many diffs)
12 human 55738 ARFGAP1 ADP ribosylation factor GTP... XR_936576.2 34.7% (many diffs)
13 human 55738 ARFGAP1 ADP ribosylation factor GTP... XR_936574.2 31.7% (many diffs)
14 human 55738 ARFGAP1 ADP ribosylation factor GTP... NR_104022.2 20.3% (many diffs)
15 mouse 228998 Arfgap1 ADP-ribosylation factor GTP... XM_006500594.3 83.4% 84.9% (many diffs)
16 mouse 228998 Arfgap1 ADP-ribosylation factor GTP... XM_011239935.1 83.4% 84.9% (many diffs)
17 mouse 228998 Arfgap1 ADP-ribosylation factor GTP... NM_145760.3 81.3% 83% (many diffs)
18 mouse 228998 Arfgap1 ADP-ribosylation factor GTP... XM_017318014.1 81.3% 83% (many diffs)
19 mouse 228998 Arfgap1 ADP-ribosylation factor GTP... NM_001177707.1 77.6% 79.1% (many diffs)
20 mouse 228998 Arfgap1 ADP-ribosylation factor GTP... NM_001177708.1 77.6% 79.1% (many diffs)
21 mouse 228998 Arfgap1 ADP-ribosylation factor GTP... NM_001177706.1 77.2% 78.9% (many diffs)
22 mouse 228998 Arfgap1 ADP-ribosylation factor GTP... NM_001177709.1 77.2% 78.9% (many diffs)
23 mouse 228998 Arfgap1 ADP-ribosylation factor GTP... NM_001177710.1 69.2% 69.8% (many diffs)
24 mouse 228998 Arfgap1 ADP-ribosylation factor GTP... XM_011239936.1 57.8% 52.5% (many diffs)
25 mouse 228998 Arfgap1 ADP-ribosylation factor GTP... XR_001782401.1 40% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1308
ORF length:
1242
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cagcccaaga accaggaagg ttcttaaaga agtcagggtg caggatgaga
121acaacgtttg ttttgagtgt ggcgcgttca atcctcagtg ggtcagtgtg acctacggca
181tctggatctg cctggagtgc tcggggagac accgcgggct tggggttcac ctcagctttg
241tgcgctctgt tactatggac aagtggaagg acattgagct tgagaagatg aaagctggtg
301ggaatgctaa gttccgagag ttcctggagt ctcaggagga ttacgatcct tgctggtcct
361tgcaggagaa gtacaacagc agagccgcgg ccctctttag ggataaggtg gtcgctctgg
421ccgaaggcag agagtggtct ctggagtcat cacctgccca gaactggacc ccacctcagc
481ccaggacgct gccgtccatg gtgcaccgag tctctggcca gccgcagagt gtgaccgcct
541cctcggacaa ggcttttgaa gactggctga atgatgacct cggctcctat caaggggccc
601aggggaatcg ctacgtgggg tttgggaaca cgccaccgcc tcagaagaaa gaagatgact
661tcctcaacaa cgccatgtcc tccctgtact cgggctggag cagcttcacc actggagcca
721gccggtttgc ctcggcagcc aaggagggcg ctacaaagtt tggatcccaa gcgagtcaga
781agttttgggg tcacaagcag cagccggagc cggcgtccga gctgggccac agcctgaacg
841agaacgtcct caagcctgcg caggagaagg tgaaggaggg aaagattttt gatgatgtct
901ccagtggggt ctctcagttg gcgtccaagg gagtcggtag taagggatgg cgggacgtca
961ccaccttttt ttcggggaaa gcagagggcc ccttggacag cccctcggag ggccacagtt
1021atcagaacag cggtctggac cacttccaaa acagcaacat agaccagagc ttctgggaga
1081cctttggaag tgctgagccc accaagaccc gcaagtcccc gagcagcgac agctggacgt
1141gcgcggacac ctccaccgag aggaggagct cggacagctg ggaggtgtgg ggctcggcct
1201ccaccaacag gaacagcaac agcgacggcg gggagggcgg ggagggcacc aagaaggcag
1261tgccgccggc cgtgcccact gatgatggct gggacaacca gaactggtac ccaactttct
1321tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1381aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)