Construct: ORF ccsbBroad304_04899
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006941.1_s304c1, BRDN0000407853
- Derived from:
- ccsbBroadEn_04899
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PACRG (135138)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04899
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 135138 | PACRG | parkin coregulated | NM_001080378.1 | 100% | 100% | |
| 2 | human | 135138 | PACRG | parkin coregulated | NM_001080379.1 | 100% | 100% | |
| 3 | human | 135138 | PACRG | parkin coregulated | NM_152410.2 | 86.8% | 86.8% | 613_729del |
| 4 | human | 135138 | PACRG | parkin coregulated | XM_017010280.2 | 84.9% | 75% | (many diffs) |
| 5 | human | 135138 | PACRG | parkin coregulated | XM_006715344.4 | 83.3% | 80.9% | (many diffs) |
| 6 | human | 135138 | PACRG | parkin coregulated | XM_011535462.3 | 82.7% | 69.7% | (many diffs) |
| 7 | human | 135138 | PACRG | parkin coregulated | XM_011535461.3 | 82.7% | 75% | (many diffs) |
| 8 | human | 135138 | PACRG | parkin coregulated | XM_005266826.5 | 81.2% | 80.2% | (many diffs) |
| 9 | human | 135138 | PACRG | parkin coregulated | XM_005266825.5 | 81.1% | 78.8% | (many diffs) |
| 10 | human | 135138 | PACRG | parkin coregulated | XM_006715345.4 | 80.2% | 80.5% | (many diffs) |
| 11 | human | 135138 | PACRG | parkin coregulated | XM_011535469.3 | 80.1% | 80.1% | 612_613insGTGAACTCCGGAGAC;618_619ins138 |
| 12 | human | 135138 | PACRG | parkin coregulated | XM_011535468.3 | 79.8% | 79.3% | 614_615delGC;618_622delGGCTCinsAA;627_628ins149 |
| 13 | human | 135138 | PACRG | parkin coregulated | XM_011535466.2 | 79% | 79.3% | (many diffs) |
| 14 | human | 135138 | PACRG | parkin coregulated | XM_017010275.2 | 71.5% | 57.2% | (many diffs) |
| 15 | human | 135138 | PACRG | parkin coregulated | XM_017010276.2 | 71.5% | 57.2% | (many diffs) |
| 16 | human | 135138 | PACRG | parkin coregulated | XM_017010277.2 | 71.5% | 57.2% | (many diffs) |
| 17 | human | 135138 | PACRG | parkin coregulated | XM_017010278.2 | 71.5% | 57.2% | (many diffs) |
| 18 | human | 135138 | PACRG | parkin coregulated | XM_017010279.2 | 71.5% | 57.2% | (many diffs) |
| 19 | human | 135138 | PACRG | parkin coregulated | XM_011535471.2 | 55.8% | 39.2% | (many diffs) |
| 20 | human | 135138 | PACRG | parkin coregulated | XM_017010281.2 | 36.9% | 25.4% | (many diffs) |
| 21 | human | 135138 | PACRG | parkin coregulated | XM_017010282.2 | 36.9% | 25.4% | (many diffs) |
| 22 | mouse | 69310 | Pacrg | PARK2 co-regulated | NM_027032.2 | 83.2% | 86.3% | (many diffs) |
| 23 | mouse | 69310 | Pacrg | PARK2 co-regulated | XM_006523353.3 | 47.9% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 837
- ORF length:
- 771
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ggcagaaaaa gagaccctga gcttaaacaa atgcccagac aagatgccga 121 agaggaccaa gctgctggca caacagccgc tcccggtgca ccagcctcac tctctggttt 181 ctgagggttt cacagtcaaa gccatgatga aaaactcagt cgtgagaggc cctccagctg 241 caggggcatt taaagaaaga ccaaccaagc ccacagcatt tcgaaaattc tatgagcgag 301 gtgacttccc aattgccctt gagcatgatt cgaaaggaaa caaaatcgcc tggaaggtag 361 aaattgagaa gctggattac catcattatc tgcctctgtt ttttgatggg ctttgtgaaa 421 tgacatttcc ctatgagttt tttgctcggc aaggaatcca cgacatgctg gaacacggtg 481 ggaacaagat cctacctgtc cttccacagc tcattatccc gataaaaaat gccttgaacc 541 tccgaaaccg acaggtcatc tgtgtcactc tcaaggtcct ccagcatctg gttgtgtcag 601 ctgagatggt gggcaaggcc ttggtgcctt attaccgtca aatcctccct gtcctgaaca 661 tctttaagaa tatgaatgtg aactccggag acggcattga ctacagccag cagaagaggg 721 agaacattgg ggacttgatc caggagacac tggaggcctt cgagcgctac ggaggagaaa 781 atgcctttat caacattaag tacgtggtcc caacctacga gtcttgcttg ctaaactacc 841 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 901 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 961 tta