Transcript: Mouse XM_006523353.3

PREDICTED: Mus musculus PARK2 co-regulated (Pacrg), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pacrg (69310)
Length:
1007
CDS:
279..698

Additional Resources:

NCBI RefSeq record:
XM_006523353.3
NBCI Gene record:
Pacrg (69310)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006523353.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000189879 CCTCCGAAACAGACAGATCAT pLKO.1 398 CDS 100% 4.950 3.465 N Pacrg n/a
2 TRCN0000191049 CCTGAACATCTTTAAGAACAT pLKO.1 512 CDS 100% 4.950 3.465 N Pacrg n/a
3 TRCN0000217411 GTCCTAGCATCTCATTACTTG pLKO.1 753 3UTR 100% 4.950 3.465 N Pacrg n/a
4 TRCN0000189976 GATCATCTGTGTGACGCTCAA pLKO.1 413 CDS 100% 4.050 2.835 N Pacrg n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006523353.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04899 pDONR223 100% 47.9% 50.5% None (many diffs) n/a
2 ccsbBroad304_04899 pLX_304 0% 47.9% 50.5% V5 (many diffs) n/a
3 TRCN0000469772 GCCCGCTGGTAGACAACCTGAAAC pLX_317 58.7% 47.9% 50.5% V5 (many diffs) n/a
4 ccsbBroadEn_04898 pDONR223 100% 47.9% 50.5% None (many diffs) n/a
5 ccsbBroad304_04898 pLX_304 0% 47.9% 50.5% V5 (many diffs) n/a
6 TRCN0000470221 AAGGAGTTCCTCTCAACATAGACA pLX_317 58% 47.9% 50.5% V5 (many diffs) n/a
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