Construct: ORF ccsbBroad304_05736

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015344.1_s304c1, BRDN0000408404
Derived from:
ccsbBroadEn_05736
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GAGE12B (729428)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05736
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 729422 GAGE12C G antigen 12C NM_001098408.1 100% 100%
2 human 729431 GAGE12E G antigen 12E NM_001098418.1 100% 100%
3 human 100132399 GAGE12D G antigen 12D NM_001127199.1 100% 100%
4 human 100008586 GAGE12F G antigen 12F NM_001098405.2 99.7% 99.1% 148C>T
5 human 645073 GAGE12G G antigen 12G NM_001098409.2 99.7% 99.1% 148C>T
6 human 729442 GAGE12H G antigen 12H NM_001098410.1 99.7% 99.1% 172A>G
7 human 729428 GAGE12B G antigen 12B NM_001127345.2 99.7% 99.1% 148C>T
8 human 26748 GAGE12I G antigen 12I NM_001477.1 99.7% 99.1% 148C>T
9 human 2579 GAGE7 G antigen 7 NM_021123.3 99.7% 99.1% 148C>T
10 human 2576 GAGE4 G antigen 4 NM_001474.1 99.4% 98.2% 148C>T;223G>C
11 human 2577 GAGE5 G antigen 5 NM_001475.1 99.1% 97.4% 67G>A;148C>T;223G>C
12 human 2543 GAGE1 G antigen 1 NM_001040663.2 98.8% 97.4% 148C>T;223G>C;337_338delGGinsAA
13 human 729396 GAGE12J G antigen 12J NM_001098406.2 98.8% 97.4% (many diffs)
14 human 2578 GAGE6 G antigen 6 NM_001476.1 98.8% 96.5% (many diffs)
15 human 645051 GAGE13 G antigen 13 NM_001098412.2 98.5% 97.4% (many diffs)
16 human 645037 GAGE2B G antigen 2B NM_001098411.3 97.7% 96.5% (many diffs)
17 human 2574 GAGE2C G antigen 2C NM_001472.2 97.7% 96.5% (many diffs)
18 human 729408 GAGE2D G antigen 2D NM_001098407.1 97.4% 95.7% (many diffs)
19 human 26749 GAGE2E G antigen 2E NM_001127200.3 97.4% 95.7% (many diffs)
20 human 729447 GAGE2A G antigen 2A NM_001127212.1 97.4% 95.7% (many diffs)
21 human 100101629 GAGE8 G antigen 8 NM_012196.1 97.4% 95.7% (many diffs)
22 human 102724473 GAGE10 G antigen 10 NM_001098413.3 94.8% 89.7% (many diffs)
23 human 2543 GAGE1 G antigen 1 XM_011543896.2 89.7% 88.7% (many diffs)
24 human 102724473 GAGE10 G antigen 10 XM_024452325.1 80% 71.7% (many diffs)
25 human 645073 GAGE12G G antigen 12G NM_001354422.1 63.8% 63.2% 148C>T;202_203ins126
26 human 2543 GAGE1 G antigen 1 NR_102272.1 10.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
420
ORF length:
351
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gagttggcga ggaagatcga cctattattg gcctagacca aggcgctatg
121tacagcctcc tgaaatgatt gggcctatgc ggcccgagca gttcagtgat gaagtggaac
181cagcaacacc tgaagaaggg gaaccagcaa ctcaatgtca ggatcctgca gctgctcagg
241agggagagga tgagggagca tctgcaggtc aagggccgaa gcctgaagct catagccagg
301aacagggtca cccacagact gggtgtgagt gtgaagatgg tcctgatggg caggagatgg
361acccgccaaa tccagaggag gtgaaaacgc ctgaagaagg tgaaaagcaa tcacagtgtt
421tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
481attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
541ggatta

Download FASTA (ORF) (Full)