Construct: ORF ccsbBroad304_05779

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016215.1_s304c1, BRDN0000405067
Derived from:
ccsbBroadEn_05779
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ADK (132)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05779
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 132 ADK adenosine kinase NM_001123.3 99.9% 99.7% 604A>T
2 human 132 ADK adenosine kinase NM_006721.4 94.8% 93.9% (many diffs)
3 human 132 ADK adenosine kinase NM_001202449.1 94.6% 94.4% 89_90ins54;550A>T
4 human 132 ADK adenosine kinase NM_001369124.1 83.4% 83.4% 502_503ins171
5 human 132 ADK adenosine kinase XM_017015699.1 83.3% 76.5% (many diffs)
6 human 132 ADK adenosine kinase XM_017015702.1 80.1% 68.7% 604A>T;709_710ins115;831_832ins89
7 human 132 ADK adenosine kinase XM_017015698.1 79.2% 75.5% (many diffs)
8 human 132 ADK adenosine kinase NM_001202450.1 79.1% 78.4% (many diffs)
9 human 132 ADK adenosine kinase XM_017015703.2 78.1% 77.9% 0_1ins225;379A>T
10 human 132 ADK adenosine kinase NM_001369123.1 76% 64.4% (many diffs)
11 human 132 ADK adenosine kinase XM_017015706.1 65.7% 64.9% (many diffs)
12 human 132 ADK adenosine kinase XM_017015704.1 62.3% 60.7% (many diffs)
13 human 132 ADK adenosine kinase XM_017015705.1 60.4% 49.3% (many diffs)
14 mouse 11534 Adk adenosine kinase NM_001243041.1 89.2% 91% (many diffs)
15 mouse 11534 Adk adenosine kinase NM_134079.4 85% 86.1% (many diffs)
16 mouse 11534 Adk adenosine kinase XM_006518440.3 59% 56.1% (many diffs)
17 mouse 11534 Adk adenosine kinase XM_006518441.3 55.4% 55.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1101
ORF length:
1035
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61aagccatgac gtcagtcaga gaaaatattc tctttggaat gggaaatcct ctgcttgaca
121tctctgctgt agtggacaaa gatttccttg ataagtattc tctgaaacca aatgaccaaa
181tcttggctga agacaaacac aaggaactgt ttgatgaact tgtgaaaaaa ttcaaagtcg
241aatatcatgc tggtggctct acccagaatt caattaaagt ggctcagtgg atgattcaac
301agccacacaa agcagcaaca ttttttggat gcattgggat agataaattt ggggagatcc
361tgaagagaaa agctgctgaa gcccatgtgg atgctcatta ctacgagcag aatgagcagc
421caacaggaac ttgtgctgca tgcatcactg gtgacaacag gtccctcata gctaatcttg
481ctgctgccaa ttgttataaa aaggaaaaac atcttgatct ggagaaaaac tggatgttgg
541tagaaaaagc aagagtttgt tatatagcag gcttttttct tacagtttcc ccagagtcag
601tattaaaggt ggctcaccat gcttctgaaa acaacaggat tttcactttg aatctatctg
661caccgttttt tagccagttc tacaaggaat cattgatgaa agttatgcct tatgttgata
721tactttttgg aaatgagaca gaagctgcca cttttgctag agagcaaggc tttgagacta
781aagacattaa agagatagcc aaaaagacac aagccctgcc aaagatgaac tcaaagaggc
841agcgaatcgt gatcttcacc caagggagag atgacactat aatggctaca gaaagtgaag
901tcactgcttt tgctgtcttg gatcaagacc agaaagaaat tattgatacc aatggagctg
961gagatgcatt tgttggaggt tttctgtctc aactggtctc tgacaagcct ctgactgaat
1021gtatccgtgc tggccactat gcagcaagca tcataattag acggactggc tgcacctttc
1081ctgagaagcc agacttccac tgcccaactt tcttgtacaa agtggttggt aagcctatcc
1141ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1201ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)