Construct: ORF ccsbBroad304_05976
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003004.1_s304c1, BRDN0000406941
- Derived from:
- ccsbBroadEn_05976
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDH13 (1012)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05976
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1012 | CDH13 | cadherin 13 | NM_001257.5 | 99.7% | 99.4% | (many diffs) |
| 2 | human | 1012 | CDH13 | cadherin 13 | NM_001220489.2 | 94.2% | 93.9% | (many diffs) |
| 3 | human | 1012 | CDH13 | cadherin 13 | NM_001220488.2 | 93.5% | 91.6% | (many diffs) |
| 4 | human | 1012 | CDH13 | cadherin 13 | XM_011522804.3 | 84.6% | 82.3% | (many diffs) |
| 5 | human | 1012 | CDH13 | cadherin 13 | NM_001220490.2 | 64.1% | 63.9% | (many diffs) |
| 6 | human | 1012 | CDH13 | cadherin 13 | XM_017022848.2 | 42.4% | 40.2% | (many diffs) |
| 7 | human | 1012 | CDH13 | cadherin 13 | NM_001220491.2 | 25.2% | 23.6% | (many diffs) |
| 8 | human | 1012 | CDH13 | cadherin 13 | XM_017022849.2 | 23.7% | 20.6% | (many diffs) |
| 9 | human | 1012 | CDH13 | cadherin 13 | NM_001220492.2 | 22.9% | 22.2% | (many diffs) |
| 10 | mouse | 12554 | Cdh13 | cadherin 13 | NM_019707.5 | 87% | 92.8% | (many diffs) |
| 11 | mouse | 12554 | Cdh13 | cadherin 13 | XM_006530628.3 | 85.3% | 91% | (many diffs) |
| 12 | mouse | 12554 | Cdh13 | cadherin 13 | XM_017312550.1 | 84.4% | 89.7% | (many diffs) |
| 13 | mouse | 12554 | Cdh13 | cadherin 13 | XM_011248295.1 | 82.9% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2205
- ORF length:
- 2139
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca gccgagaact ccgctcgttc tgtgcgttct cctgtcccag gtgctgctgc 121 taacatctgc agaagatttg gactgcactc ctggatttca gcagaaagtg ttccatatca 181 atcagccagc tgaattcatt gaggaccagt caattctaaa cttgaccttc agtgactgta 241 agggaaacga caagctacgc tatgaggtct cgagcccata cttcaaggtg aacagcgatg 301 gcggcttagt tgctctgaga aacataactg cagtgggcaa aactctgttc gtccatgcac 361 ggacccccca tgcggaagat atggcagaac tcgtgattgt cggggggaaa gacatccagg 421 gctccttgca ggatatattt aaatttgcaa gaacttctcc tgtcccaaga caaaagaggt 481 ccattgtggt atctcccatt ttaattccag agaatcagag acagcctttc ccaagagatg 541 ttggcaaggt agtcgatagt gacaggccag aaaggtccaa gttccggctc actggaaagg 601 gagtggatca agagcctaaa ggaattttca gaatcaatga gaacacaggg agcgtctcca 661 tgacacggac cttggacaga gaagtaatcg ctgtttatca actatttgtg gagaccactg 721 atgtcaatgg caaaactctc gaggggccgg tgcctctgga agtcattgtg attgatcaga 781 atgacaaccg accgatcttt cgggaaggcc cctacatcgg ccacgtcatg gaagggtcac 841 ccacaggcac cacagtgatg cggatgacag cctttgatgc agatgaccca gccaccgata 901 atgccctcct gcggtataat atccgtcggc agacgcctga caagccatct cccaacatgt 961 tctacatcga tcctgagaaa ggagacattg tcactgttgt gtcacctgcg ctgctggacc 1021 gagagactct ggaaaatccc aagtatgaac tgatcatcga ggctcaagat atggctggac 1081 tggatgttgg attaacaggc acggccacag ccacgatcat gatcgatgac aaaaatgatc 1141 actcaccaaa attcaccaag aaagagtttc aagccacagt cgaggaagga gctgtgggag 1201 ttattgtcaa tttgacagtt gaagataagg atgaccccgc cacaggtgca tggagggctg 1261 cctacaccat catcaacgga aaccccgggc agagctttga aatccacacc aaccctcaaa 1321 ccaacgaagg gatgctttct gttgtcaaac cattggacta tgaaatttct gccttccaca 1381 ccctgctgat caaagtggaa aatgaagacc cactcgtacc cgacgtctcc tacggcccca 1441 gctccacagc caccgtccac atcactgtcc tggatgtcaa cgagggccca gtcttctacc 1501 cagaccccat gatggtgacc aggcaggagg acctctctgt gggcagcgtg ctgctgacag 1561 tgaatgccac ggaccccgac tccctgcagc atcaaaccat caggtattct gtttacaagg 1621 acccagcagg ttggctgaat attaacccca tcaatgggac tgttgacacc acagctgtgc 1681 tggaccgtga gtccacattt gtcgacaaca gcgtgtacac tgctctcttc ctggcaattg 1741 acagtggcaa ccctcccgct acgggcactg ggactttgct gataaccctg gaggacgtga 1801 atgacaatgc cccgttcatt taccccacag tagctgaagt ctgtgatgat gccaaaaacc 1861 tcagtgtagt cattttggga gcatcagata aggatcttca cccgaataca gatcctttca 1921 aatttgaaat ccacaaacaa gctgttcctg ataaagtctg gaagatctcc aagatcaaca 1981 atacacacgc cctggtaagc cttcttcaaa atctgaacaa agcaaactac aacctgccca 2041 tcatggtgac agattcaggg aaaccaccca tgacgaatat cacagatctc agggtacaag 2101 tgtgctcctg caggaattcc aaagtggact gcaacgcggc gggggccctg cgcttcagcc 2161 tgccctcagt cctgctcctc agcctcttca gcttagcttg tctgtgccca actttcttgt 2221 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 2281 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a