Construct: ORF ccsbBroad304_05978

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF001277.1_s304c1, BRDN0000400362
• Derived from: ccsbBroadEn_05978
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: CDH18 (1016)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_05978
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	1016	CDH18	cadherin 18	NM_001291956.3	99.7%	99.3%	(many diffs)
2	human	1016	CDH18	cadherin 18	NM_001349556.2	99.7%	99.3%	(many diffs)
3	human	1016	CDH18	cadherin 18	NM_001349558.2	99.7%	99.3%	(many diffs)
4	human	1016	CDH18	cadherin 18	NM_001349559.2	99.7%	99.3%	(many diffs)
5	human	1016	CDH18	cadherin 18	NM_004934.5	99.7%	99.3%	(many diffs)
6	human	1016	CDH18	cadherin 18	XM_005248228.4	99.7%	99.3%	(many diffs)
7	human	1016	CDH18	cadherin 18	XM_006714435.4	99.7%	99.3%	(many diffs)
8	human	1016	CDH18	cadherin 18	XM_017008924.2	99.7%	99.3%	(many diffs)
9	human	1016	CDH18	cadherin 18	XM_017008926.2	99.7%	99.3%	(many diffs)
10	human	1016	CDH18	cadherin 18	XM_017008927.2	99.7%	99.3%	(many diffs)
11	human	1016	CDH18	cadherin 18	XM_017008928.2	99.7%	99.3%	(many diffs)
12	human	1016	CDH18	cadherin 18	XM_017008929.2	99.7%	99.3%	(many diffs)
13	human	1016	CDH18	cadherin 18	XM_017008930.2	99.7%	99.3%	(many diffs)
14	human	1016	CDH18	cadherin 18	XM_011513928.3	91.1%	90%	(many diffs)
15	human	1016	CDH18	cadherin 18	XM_011513929.3	78.1%	77.7%	(many diffs)
16	human	1016	CDH18	cadherin 18	NM_001291957.2	72.5%	67.5%	(many diffs)
17	human	1016	CDH18	cadherin 18	NM_001349560.2	72.5%	67.5%	(many diffs)
18	human	1016	CDH18	cadherin 18	XM_011513930.3	72.5%	67.5%	(many diffs)
19	human	1016	CDH18	cadherin 18	NM_001167667.3	72.4%	68.2%	(many diffs)
20	human	1016	CDH18	cadherin 18	NM_001349561.2	72.4%	68.2%	(many diffs)
21	human	1016	CDH18	cadherin 18	NM_001349562.2	71.5%	71.2%	(many diffs)
22	human	1016	CDH18	cadherin 18	NM_001349563.2	71.5%	71.2%	(many diffs)
23	mouse	320865	Cdh18	cadherin 18	NM_001081299.1	89%	95.5%	(many diffs)
24	mouse	320865	Cdh18	cadherin 18	XM_006520094.3	89%	95.5%	(many diffs)
25	mouse	320865	Cdh18	cadherin 18	XM_006520095.3	89%	95.5%	(many diffs)
26	mouse	320865	Cdh18	cadherin 18	XM_006520096.3	89%	95.5%	(many diffs)
27	mouse	320865	Cdh18	cadherin 18	XM_011245360.2	89%	95.5%	(many diffs)
28	mouse	320865	Cdh18	cadherin 18	XM_006520097.3	64.6%	65.4%	(many diffs)
29	mouse	320865	Cdh18	cadherin 18	XM_006520098.3	64.5%	65.3%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

2436

ORF length:

2370

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatgaa aattacttgc acatcttgca tctgtccagt cctagtgtgt ctctgttttg
121	tgcagaggtg ttatggaact gctcaccaca gctccatcaa ggtgatgaga aaccaaacca
181	aacacattga aggtgaaacc gaagtccatc atcgtcccaa aaggggatgg gtatggaatc
241	agttctttgt tttagaagaa catatgggac cagatcctca gtatgttgga aagctgcact
301	ccaattctga caaaggtgat ggatctgtca agtacatcct tactggagag ggtgctggga
361	ctatatttat cattgacgat accacgggtg atatccactc aacaaaaagc ctagacagag
421	agcagaagac ccactatgtg cttcatgctc aagctattga tagacgtaca aacaaacctc
481	ttgagcctga atccgagttc atcatcaaag tgcaagacat caatgacaac gctccaaaat
541	tcacagatgg accatacatt gttactgtgc ctgaaatgtc agatatgggt acctctgttc
601	tacaggtgac agctactgat gcagatgacc ctacctatgg aaacagcgct cgggtggttt
661	acagcattct ccagggacaa ccctacttct ccgtcgaccc taaaacagga gttattagaa
721	cggccttaca taacatggac ggagaagcca gagaacatta ctccgtagtc attcaagcca
781	aagacatggc tgggcaagtt ggagggcttt caggatctac aacagtcaac atcaccttaa
841	ccgatgtcaa tgacaacaca ccacgctttc ctcaaaaaca ctatcagcta tatgttcctg
901	agtcagctca agttggttca gctgttggga aaatcaaggc aaatgatgct gacactggct
961	caaatgctga catgacctac tccatcataa atggtgatgg catgggaata ttctcaatct
1021	ccactgacaa agagaccaga gaaggaatcc tttctttaaa gaagccactg aactatgaga
1081	aaaagaagtc atataccctc aacatagaag gagcaaatac acatcttgat tttcgctttt
1141	ctcacttggg tccttttaaa gatgctacta tgctgaagat cattgttggg gatgtagatg
1201	aaccaccact attttccatg ccttcctacc tcatggaagt ctacgaaaat gccaagattg
1261	ggaccgtcgt tggtacagtt ttggcacaag atcctgacag tattaacagc ttagtaagat
1321	acttcatcaa ctacaatgtt gaagacgaca gatttttcaa cattgatgcc aatactggga
1381	ccattaggac tacaaaggtt ctcgacagag aagaaactcc atggtacaac atcacagtca
1441	ctgcttcaga aattgataat cctgatttgc tgagccatgt cacagtgggt attagagttc
1501	tggatgtcaa tgacaatcca cccgaacttg ccagggaata tgatattatt gtatgtgaaa
1561	attctaagcc tggccaggtt attcatacca tcagtgccac tgataaagat gattttgcca
1621	atggaccaag gtttaacttc tttcttgatg agcgcctgcc tgtaaatcca aacttcactc
1681	tgaaggacaa tgaagataac acagccagca ttctgacaag gcggaggaga tttagtcgaa
1741	ctgttcagga tgtgtattat ctgcccatta tgatctctga tggtggaatc ccctctctca
1801	gcagcagcag caccctcacc atcagggttt gtgcatgcga gagagatggg cgtgtgcgga
1861	cctgccatgc agaagccttc ctgtcctcgg ctggtttgag tacaggagcc ttaatcgcta
1921	ttcttctctg tgttctcatt ctcctggcaa ttgtggtact ttttatcacc ctgaggcgca
1981	gcaaaaaaga gcccttgatc atttcagaag aggatgtacg ggagaacgtg gtcacctatg
2041	atgatgaagg aggcggagag gaagacacag gggcctttga catcacagcc ttgaggaatc
2101	cttctgctgc tgaggagctc aagtaccgga gggatatcag acctgaagtg aagctcactc
2161	ccagacacca gacatcatcc accctggaaa gcatagatgt tcaggaattt attaagcaaa
2221	gactggcaga agcagaccta gaccctagcg ttccccctta tgactctctt cagacttatg
2281	cctatgaggg tcagagatca gaagctgggt ctatcagctc gctggattca gcaacgacac
2341	aatcagacca ggattatcac taccttggag actggggacc cgagtttaaa aagttagctg
2401	aactctatgg agaaatagaa tctgaaagaa caactcaccc aactttcttg tacaaagtgg
2461	ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
2521	ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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