Construct: ORF ccsbBroad304_06279

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006428.1_s304c1, BRDN0000406795
Derived from:
ccsbBroadEn_06279
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GK2 (2712)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06279
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2712 GK2 glycerol kinase 2 NM_033214.3 99.9% 100% 12A>G
2 human 2710 GK glycerol kinase NM_001128127.2 88.1% 88.4% (many diffs)
3 human 2710 GK glycerol kinase NM_001205019.1 87.2% 87.4% (many diffs)
4 human 2710 GK glycerol kinase XM_006724483.2 83.8% 84.1% (many diffs)
5 human 2710 GK glycerol kinase NM_000167.5 83.4% 83.3% (many diffs)
6 human 2710 GK glycerol kinase XM_011545491.2 83% 83.3% (many diffs)
7 human 2710 GK glycerol kinase NM_203391.3 82.6% 82.4% (many diffs)
8 human 2710 GK glycerol kinase XM_006724484.2 79.4% 79.3% (many diffs)
9 human 2710 GK glycerol kinase XM_011545492.1 78.7% 78.5% (many diffs)
10 human 2713 GK3P glycerol kinase 3 pseudogene NR_026575.1 64.9% (many diffs)
11 human 2710 GK glycerol kinase XM_005274488.4 55% 55.8% (many diffs)
12 human 2710 GK glycerol kinase XM_006724485.2 54.5% 55.2% (many diffs)
13 human 2710 GK glycerol kinase XM_006724486.3 54.5% 55.2% (many diffs)
14 human 2710 GK glycerol kinase XM_011545493.2 54.5% 55.2% (many diffs)
15 human 2710 GK glycerol kinase XM_011545494.2 54.5% 55.2% (many diffs)
16 human 2710 GK glycerol kinase XM_017029409.1 54.5% 55.2% (many diffs)
17 human 2710 GK glycerol kinase XM_017029411.1 50.3% 50.8% (many diffs)
18 human 2710 GK glycerol kinase XM_017029412.2 50.3% 50.8% (many diffs)
19 human 2710 GK glycerol kinase XM_017029410.1 49.9% 50.2% (many diffs)
20 mouse 14933 Gk glycerol kinase NM_212444.2 86.9% 87.8% (many diffs)
21 mouse 14933 Gk glycerol kinase NM_001294140.1 86% 86.9% (many diffs)
22 mouse 14933 Gk glycerol kinase XM_006527831.3 82.7% 83.6% (many diffs)
23 mouse 14933 Gk glycerol kinase NM_008194.3 82.3% 82.8% (many diffs)
24 mouse 14933 Gk glycerol kinase XM_006527829.3 81.9% 82.7% (many diffs)
25 mouse 14933 Gk glycerol kinase NM_001331046.1 81.4% 81.9% (many diffs)
26 mouse 14933 Gk glycerol kinase NM_001294138.1 78.3% 78.8% (many diffs)
27 mouse 14933 Gk glycerol kinase XM_006527833.3 77.6% 78% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1725
ORF length:
1659
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agccccgaag acagcagctg tggggccgtt ggtgggagcg gtggtccagg
121gcaccaactc cactcgcttt ctggttttca attcaaaaac agcggaacta cttagtcatc
181acaaagtgga attaacacaa gagttcccaa aagaaggatg ggtggaacaa gaccctaaag
241aaattcttca gtctgtctac gagtgtatag cgagaacgtg tgagaaactt gacgaactga
301atattgatat atccaacata aaagctgttg gtgtcagcaa tcagagggaa accactgtaa
361tctgggacaa gttaacagga gagcctctct acaatgctgt ggtgtggctt gatctaagaa
421cccagactac tgttgaggat cttagtaaaa aaattccagg aaatagtaac ttcgtcaagt
481ctaagacagg ccttccactc agcacttact tcagtgcagt aaaacttcgt tggatgcttg
541acaatgtgag aaacgtccaa aaggctgttg aagaaggtag agctcttttt ggtaccattg
601attcatggct tatctggagt ttgacaggag gagttaatgg aggcgtgcat tgtacagatg
661taacaaatgc aagtaggaca atgcttttta atatccattc tttggaatgg gataaagagc
721tctgtgactt ttttgaaatt ccaatggacc ttcttccaaa tgtcttcagt tcttctgaga
781tctatggcct aattaaaact ggagccctgg aaggtgtgcc aatatctggg tgtttggggg
841accaatgtgc tgcattagta ggacaaatgt gcttccagga gggacaagcc aaaaacacct
901atggaacagg ttgcttctta ctgtgtaata cgggtcgtaa atgtgtgttt tctgaacatg
961gccttttgac cacagtagct tacaaactag gcagagagaa gccagcatat tatgcactgg
1021aaggttctgt tgctatagca ggtgctgtta ttcgttggct aagagacaat cttggaatta
1081tagagacctc aggagacatt gaaagacttg ctaaagaagt aggaacttct tatggctgtt
1141actttgtccc agccttttca gggttatatg caccttattg ggagcccagt gcaagaggga
1201tactctgtgg cctcactcag tttaccaata aatgtcatat tgcttttgct gcattagaag
1261ctgtttgttt ccaaacccga gagattttgg aagccatgaa ccgtgactgt ggaattccac
1321ttcgtcattt gcaggtagat ggaggaatga ccaacaacaa agttcttatg cagctacaag
1381cagatattct tcatattcca gtaataaaac cctttatgcc tgaaacaact gcactaggag
1441ctgccatggc agcaggggct gcagagggag taagcgtttg gagccttgaa ccccaggctt
1501tgtcagttct caggatggaa cgatttgaac cacagatcca ggccacagaa agtgaaattc
1561gttatgccac atggaagaaa gccgtaatga agtcaatggg ttgggttacc agtcagtctc
1621ctgaaggtgg tgatccttct atcttctcta gtctgccttt gggatttttt atagtgagta
1681gcatggtaat gctaattgga gcaagatata tctcgggtgt gccataccca actttcttgt
1741acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1801gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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