Transcript: Human NM_033214.3

Homo sapiens glycerol kinase 2 (GK2), mRNA.

Source:
NCBI, updated 2019-06-30
Taxon:
Homo sapiens (human)
Gene:
GK2 (2712)
Length:
1868
CDS:
29..1690

Additional Resources:

NCBI RefSeq record:
NM_033214.3
NBCI Gene record:
GK2 (2712)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147665 TAGCAGGTGCTGTTATTCGT pXPR_003 TGG 987 59% 1 0.6002 GK2 GK2 77909
2 BRDN0001145991 GTCCTACTAATGCAGCACAT pXPR_003 TGG 782 47% 1 0.4267 GK2 GK2 77910
3 BRDN0001487045 ATATCCAACATAAAAGCTGT pXPR_003 TGG 260 16% 1 0.1438 GK2 GK2 77911
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_033214.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000229763 ATCTCGGGTGTGCCATAATAA pLKO_005 1673 CDS 100% 15.000 21.000 N GK2 n/a
2 TRCN0000229762 GTGTAATACGGGTCGTAAATG pLKO_005 886 CDS 100% 13.200 18.480 N GK2 n/a
3 TRCN0000199685 GCAAGATATATCTCGGGTGTG pLKO.1 1664 CDS 100% 2.250 3.150 N GK2 n/a
4 TRCN0000196302 GCAGTTCTGCCTCTTAATATA pLKO.1 1746 3UTR 100% 15.000 12.000 N GK2 n/a
5 TRCN0000257429 GACTACTGTTGAGGATCTTAG pLKO_005 388 CDS 100% 10.800 8.640 N GK2 n/a
6 TRCN0000037645 GCGTGCATTGTACAGATGTAA pLKO.1 606 CDS 100% 5.625 4.500 N GK2 n/a
7 TRCN0000358593 AGAGAGAAGCCAGCATATTAT pLKO_005 956 CDS 100% 15.000 10.500 N GK2 n/a
8 TRCN0000194910 CCTCACTCAGTTTACCAATAA pLKO.1 1174 CDS 100% 13.200 9.240 N GK2 n/a
9 TRCN0000218506 TATGGAGTCCCAAGATGTAAA pLKO_005 1701 3UTR 100% 13.200 9.240 N GK2 n/a
10 TRCN0000229761 TGACGAACTGAATATTGATAT pLKO_005 253 CDS 100% 13.200 9.240 N GK2 n/a
11 TRCN0000358654 AGTAACTTCGTCAAGTCTAAG pLKO_005 428 CDS 100% 10.800 7.560 N GK2 n/a
12 TRCN0000037648 CTTCGTCATTTGCAGGTAGAT pLKO.1 1283 CDS 100% 4.950 3.465 N GK2 n/a
13 TRCN0000196782 GAAATTCCAATGGACCTTCTT pLKO.1 698 CDS 100% 4.950 3.465 N GK2 n/a
14 TRCN0000037647 GAGAAACTTGACGAACTGAAT pLKO.1 245 CDS 100% 4.950 3.465 N GK2 n/a
15 TRCN0000037646 GTGCTGCATTAGTAGGACAAA pLKO.1 810 CDS 100% 4.950 3.465 N GK2 n/a
16 TRCN0000358615 TACATAATGAAAGCATATAGC pLKO_005 1727 3UTR 100% 4.950 3.465 N GK2 n/a
17 TRCN0000037644 CCACAGAAAGTGAAATTCGTT pLKO.1 1506 CDS 100% 3.000 2.100 N GK2 n/a
18 TRCN0000195234 CTGAGATCTATGGCCTAATTA pLKO.1 738 CDS 100% 15.000 7.500 Y GK2 n/a
19 TRCN0000024656 CCATTGATTCATGGCTTATTT pLKO.1 558 CDS 100% 15.000 9.000 N Gk n/a
20 TRCN0000319760 CCATTGATTCATGGCTTATTT pLKO_005 558 CDS 100% 15.000 9.000 N Gk n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_033214.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06279 pDONR223 100% 99.9% 100% None 12A>G n/a
2 ccsbBroad304_06279 pLX_304 0% 99.9% 100% V5 12A>G n/a
3 ccsbBroadEn_14656 pDONR223 0% 99.9% 100% None 12A>G n/a
4 ccsbBroad304_14656 pLX_304 0% 99.9% 100% V5 12A>G n/a
5 TRCN0000474895 TTTCTTGTATCACAAGGATTACCA pLX_317 31.2% 99.9% 100% V5 12A>G n/a
6 ccsbBroadEn_10490 pDONR223 100% 87.3% 87.5% None (many diffs) n/a
7 ccsbBroad304_10490 pLX_304 0% 87.3% 87.5% V5 (many diffs) n/a
8 TRCN0000479938 GTACCAGTTGGTCTCCCACTAACC pLX_317 24.1% 87.3% 87.5% V5 (many diffs) n/a
9 ccsbBroadEn_06278 pDONR223 100% 82.5% 82.1% None (many diffs) n/a
10 ccsbBroad304_06278 pLX_304 0% 82.5% 82.1% V5 (many diffs) n/a
11 TRCN0000473511 TCCACTTGCGAAGTCGGGCGCCAG pLX_317 31.9% 82.5% 82.1% V5 (many diffs) n/a
12 ccsbBroadEn_14655 pDONR223 0% 82.5% 82.1% None (many diffs) n/a
13 ccsbBroad304_14655 pLX_304 0% 82.5% 82.1% V5 (many diffs) n/a
14 TRCN0000470124 CTTTTCCAGCCGTATGCAGTACTA pLX_317 25.7% 82.5% 82.1% V5 (many diffs) n/a
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