Transcript: Mouse NM_001294138.1

Mus musculus glycerol kinase (Gk), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Gk (14933)
Length:
4441
CDS:
317..1975

Additional Resources:

NCBI RefSeq record:
NM_001294138.1
NBCI Gene record:
Gk (14933)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146383 AGGAAATAATAACTTTGTCA pXPR_003 AGG 493 30% 6 0.3828 Gk GK 76329
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001294138.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000024654 CCTGTGTATTATGCGTTGGAA pLKO.1 1337 CDS 100% 3.000 2.400 N Gk n/a
2 TRCN0000319734 CCTGTGTATTATGCGTTGGAA pLKO_005 1337 CDS 100% 3.000 2.400 N Gk n/a
3 TRCN0000024656 CCATTGATTCATGGCTTATTT pLKO.1 930 CDS 100% 15.000 10.500 N Gk n/a
4 TRCN0000319760 CCATTGATTCATGGCTTATTT pLKO_005 930 CDS 100% 15.000 10.500 N Gk n/a
5 TRCN0000024657 CAGGGTTATATGCGCCTTATT pLKO.1 1494 CDS 100% 13.200 9.240 N Gk n/a
6 TRCN0000024658 CCTTCCACTTAGCACGTATTT pLKO.1 826 CDS 100% 13.200 9.240 N Gk n/a
7 TRCN0000319696 CCTTCCACTTAGCACGTATTT pLKO_005 826 CDS 100% 13.200 9.240 N Gk n/a
8 TRCN0000195270 CTTAGTCATCATCAAGTAGAA pLKO.1 422 CDS 100% 4.950 3.465 N GK n/a
9 TRCN0000024655 GCTGAACTTCTTAGTCATCAT pLKO.1 413 CDS 100% 4.950 3.465 N Gk n/a
10 TRCN0000350182 GCTGAACTTCTTAGTCATCAT pLKO_005 413 CDS 100% 4.950 3.465 N Gk n/a
11 TRCN0000195234 CTGAGATCTATGGCCTAATTA pLKO.1 1110 CDS 100% 15.000 9.000 N GK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001294138.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06278 pDONR223 100% 85.8% 91% None (many diffs) n/a
2 ccsbBroad304_06278 pLX_304 0% 85.8% 91% V5 (many diffs) n/a
3 TRCN0000473511 TCCACTTGCGAAGTCGGGCGCCAG pLX_317 31.9% 85.8% 91% V5 (many diffs) n/a
4 ccsbBroadEn_14655 pDONR223 0% 85.8% 91% None (many diffs) n/a
5 ccsbBroad304_14655 pLX_304 0% 85.8% 91% V5 (many diffs) n/a
6 TRCN0000470124 CTTTTCCAGCCGTATGCAGTACTA pLX_317 25.7% 85.8% 91% V5 (many diffs) n/a
7 ccsbBroadEn_10490 pDONR223 100% 81.6% 86.4% None (many diffs) n/a
8 ccsbBroad304_10490 pLX_304 0% 81.6% 86.4% V5 (many diffs) n/a
9 TRCN0000479938 GTACCAGTTGGTCTCCCACTAACC pLX_317 24.1% 81.6% 86.4% V5 (many diffs) n/a
10 ccsbBroadEn_06279 pDONR223 100% 78.3% 78.8% None (many diffs) n/a
11 ccsbBroad304_06279 pLX_304 0% 78.3% 78.8% V5 (many diffs) n/a
12 ccsbBroadEn_14656 pDONR223 0% 78.3% 78.8% None (many diffs) n/a
13 ccsbBroad304_14656 pLX_304 0% 78.3% 78.8% V5 (many diffs) n/a
14 TRCN0000474895 TTTCTTGTATCACAAGGATTACCA pLX_317 31.2% 78.3% 78.8% V5 (many diffs) n/a
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