Transcript: Human XM_006724485.2

PREDICTED: Homo sapiens glycerol kinase (GK), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GK (2710)
Length:
3570
CDS:
640..1704

Additional Resources:

NCBI RefSeq record:
XM_006724485.2
NBCI Gene record:
GK (2710)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006724485.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196319 GTAGGTTGAGTTCATTGTAAA pLKO.1 3410 3UTR 100% 13.200 18.480 N GK n/a
2 TRCN0000196700 GCAACGGTTATGCATAATATT pLKO.1 2573 3UTR 100% 15.000 10.500 N GK n/a
3 TRCN0000296846 GCAACGGTTATGCATAATATT pLKO_005 2573 3UTR 100% 15.000 10.500 N GK n/a
4 TRCN0000195024 CCAGCAATTCTGTCTCTTAAT pLKO.1 1759 3UTR 100% 13.200 9.240 N GK n/a
5 TRCN0000296850 CCAGCAATTCTGTCTCTTAAT pLKO_005 1759 3UTR 100% 13.200 9.240 N GK n/a
6 TRCN0000037635 ACCAGTAGTGAAGCCCTCAAT pLKO.1 1374 CDS 100% 4.950 3.465 N GK n/a
7 TRCN0000196599 GATAAACAACTCTGCGAATTT pLKO.1 670 CDS 100% 13.200 7.920 N GK n/a
8 TRCN0000296845 GATAAACAACTCTGCGAATTT pLKO_005 670 CDS 100% 13.200 7.920 N GK n/a
9 TRCN0000195040 CTTAATGCAATGACACTATTC pLKO.1 1774 3UTR 100% 10.800 6.480 N GK n/a
10 TRCN0000037634 CCGGAGTTCTTCTGAGATCTA pLKO.1 723 CDS 100% 4.950 2.970 N GK n/a
11 TRCN0000195270 CTTAGTCATCATCAAGTAGAA pLKO.1 237 5UTR 100% 4.950 2.970 N GK n/a
12 TRCN0000196785 GACTATTGATTCATGGCTTAT pLKO.1 552 5UTR 100% 10.800 5.400 Y GK n/a
13 TRCN0000037638 CTCACCACAGTGGCTTACAAA pLKO.1 943 CDS 100% 5.625 2.813 Y GK n/a
14 TRCN0000196762 GCTGAACTACTTAGTCATCAT pLKO.1 228 5UTR 100% 4.950 2.475 Y GK n/a
15 TRCN0000037636 CTAAAGAAGTAGGTACTTCTT pLKO.1 1088 CDS 100% 0.495 0.248 Y GK n/a
16 TRCN0000195234 CTGAGATCTATGGCCTAATTA pLKO.1 734 CDS 100% 15.000 7.500 Y GK2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006724485.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10490 pDONR223 100% 60.8% 61% None (many diffs) n/a
2 ccsbBroad304_10490 pLX_304 0% 60.8% 61% V5 (many diffs) n/a
3 TRCN0000479938 GTACCAGTTGGTCTCCCACTAACC pLX_317 24.1% 60.8% 61% V5 (many diffs) n/a
4 ccsbBroadEn_06278 pDONR223 100% 57.9% 57.9% None 0_1ins615;970_972delGACinsATT;975_1062delinsA n/a
5 ccsbBroad304_06278 pLX_304 0% 57.9% 57.9% V5 0_1ins615;970_972delGACinsATT;975_1062delinsA n/a
6 TRCN0000473511 TCCACTTGCGAAGTCGGGCGCCAG pLX_317 31.9% 57.9% 57.9% V5 0_1ins615;970_972delGACinsATT;975_1062delinsA n/a
7 ccsbBroadEn_14655 pDONR223 0% 57.9% 57.9% None 0_1ins615;970_972delGACinsATT;975_1062delinsA n/a
8 ccsbBroad304_14655 pLX_304 0% 57.9% 57.9% V5 0_1ins615;970_972delGACinsATT;975_1062delinsA n/a
9 TRCN0000470124 CTTTTCCAGCCGTATGCAGTACTA pLX_317 25.7% 57.9% 57.9% V5 0_1ins615;970_972delGACinsATT;975_1062delinsA n/a
10 ccsbBroadEn_06279 pDONR223 100% 54.5% 55.2% None (many diffs) n/a
11 ccsbBroad304_06279 pLX_304 0% 54.5% 55.2% V5 (many diffs) n/a
12 ccsbBroadEn_14656 pDONR223 0% 54.5% 55.2% None (many diffs) n/a
13 ccsbBroad304_14656 pLX_304 0% 54.5% 55.2% V5 (many diffs) n/a
14 TRCN0000474895 TTTCTTGTATCACAAGGATTACCA pLX_317 31.2% 54.5% 55.2% V5 (many diffs) n/a
Download CSV

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.