Construct: ORF ccsbBroad304_07396
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003945.1_s304c1, BRDN0000408335
- Derived from:
- ccsbBroadEn_07396
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDST3 (9348)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07396
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | NM_004784.3 | 99.9% | 100% | 81C>T |
| 2 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | XM_006714416.3 | 99.9% | 100% | 81C>T |
| 3 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | XM_017008839.2 | 96.7% | 94% | 81C>T;2502_2503insAGCAG;2615_2697del |
| 4 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | XM_017008840.2 | 96.7% | 94% | 81C>T;2502_2503insAGCAG;2615_2697del |
| 5 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | XM_011532415.3 | 59.7% | 58.8% | (many diffs) |
| 6 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | XM_011532416.3 | 59% | 58.7% | (many diffs) |
| 7 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | XM_017008842.1 | 56.4% | 56.4% | 0_1ins1140 |
| 8 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | XM_017008841.1 | 54.5% | 51.7% | 0_1ins1140;1362_1363insAGCAG;1475_1557del |
| 9 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | XM_017008844.1 | 54.5% | 51.7% | 0_1ins1140;1362_1363insAGCAG;1475_1557del |
| 10 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | XM_017008843.1 | 50.6% | 47.8% | 0_1ins1245;1257_1258insAGCAG;1370_1452del |
| 11 | human | 64579 | NDST4 | N-deacetylase and N-sulfotr... | XM_017008545.2 | 45.6% | 49.9% | (many diffs) |
| 12 | human | 64579 | NDST4 | N-deacetylase and N-sulfotr... | XM_017008546.1 | 45.6% | 49.9% | (many diffs) |
| 13 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | NR_146514.2 | 44.3% | (many diffs) | |
| 14 | human | 9348 | NDST3 | N-deacetylase and N-sulfotr... | NR_146513.2 | 40.9% | (many diffs) | |
| 15 | mouse | 83398 | Ndst3 | N-deacetylase/N-sulfotransf... | NM_031186.3 | 89% | 93.4% | (many diffs) |
| 16 | mouse | 83398 | Ndst3 | N-deacetylase/N-sulfotransf... | XM_006502370.2 | 89% | 93.4% | (many diffs) |
| 17 | mouse | 83398 | Ndst3 | N-deacetylase/N-sulfotransf... | XM_006502371.3 | 89% | 93.4% | (many diffs) |
| 18 | mouse | 83398 | Ndst3 | N-deacetylase/N-sulfotransf... | XM_006502372.2 | 52.6% | 54.1% | (many diffs) |
| 19 | mouse | 83398 | Ndst3 | N-deacetylase/N-sulfotransf... | XM_006502374.2 | 51.9% | 53.9% | (many diffs) |
| 20 | mouse | 83398 | Ndst3 | N-deacetylase/N-sulfotransf... | NM_001293682.1 | 47.3% | 50.4% | (many diffs) |
| 21 | mouse | 83398 | Ndst3 | N-deacetylase/N-sulfotransf... | XM_006502376.3 | 47.3% | 50.4% | (many diffs) |
| 22 | mouse | 64580 | Ndst4 | N-deacetylase/N-sulfotransf... | XM_006501860.1 | 44.9% | 50.1% | (many diffs) |
| 23 | mouse | 83398 | Ndst3 | N-deacetylase/N-sulfotransf... | NR_121616.1 | 41% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2688
- ORF length:
- 2619
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagttttatc atgaagcttc acagacactt tcaaagaaca gtcattctgc 121 ttgccacttt ttgtatggtg agcattatta tttctgctta ctacctgtac agtggctaca 181 aacaggaaaa tgaactctct gagacggctt cagaagttga ctgtggcgac ctccaacacc 241 taccatatca actaatggaa gtgaaagcaa tgaagctttt tgatgcctca aggacagacc 301 ccacagtcct agtatttgta gagagccagt actcatctct tggtcaagac atcattatga 361 ttctagaatc aagtagattc cagtatcaca ttgaaattgc ccctggaaag ggagatctcc 421 cagtgcttat agacaaaatg aaaggcaaat acattctcat tatttatgag aatattttaa 481 agtatataaa tatggattcc tggaatcgaa gccttctaga taaatactgt gtagaatatg 541 gtgtgggtgt cattggattc cacaaaacta gtgagaagag tgtacagagc tttcagttaa 601 aaggtttccc tttttccata tatggaaatc ttgcagtaaa agattgttgt attaatcctc 661 attctccatt gattcgtgtg accaaatctt ccaagcttga aaaaggttct ttacctggaa 721 ctgactggac agtttttcag attaatcatt cagcctatca accagtaata tttgccaaag 781 taaagacccc agaaaacctt tctccttcca tctctaaagg tgctttttat gccactatta 841 tacatgacct ggggcttcat gatggaattc aaagggttct ttttggcaac aacttgaact 901 tttggctgca caagctcatc ttcatagatg ccatctcctt cttatcaggg aagaggctga 961 cattgtcctt ggacaggtac attcttgtgg atattgatga tatatttgtg ggaaaagagg 1021 gaacaagaat gaacaccaat gatgtaaagg ccctgcttga tactcagaat cttttgcgtg 1081 cacaaatcac aaattttaca ttcaacctgg gattttcagg gaaattttac catacaggaa 1141 ctgaagagga agatgaagga gatgactgtc tgttggggtc tgtggatgag ttctggtggt 1201 ttcctcacat gtggagccat atgcagcccc acctcttcca caatgagtca tctttggtgg 1261 agcagatgat tctcaacaaa aaatttgcct tagagcacgg cattccaacg gacatgggct 1321 acgctgtggc ccctcaccat tcgggcgtct accctgtaca tgttcagctt tatgaggcct 1381 ggaagaaggt ctggaatatt aaaatcacca gcactgaaga atatccacat ctgaagccag 1441 ctagataccg gaggggtttt atccacaaaa acatcatggt tctcccaaga caaacctgtg 1501 ggcttttcac tcacaccatt ttctacaaag aatatccagg gggtcctaaa gagctggata 1561 agagtatcca aggaggagaa cttttcttca ctgtcgtcct caaccctatc agcattttca 1621 tgacccattt gtccaactat gggaatgacc gactgggatt atatacattt gttaatctgg 1681 ccaactttgt gaagagctgg accaacctgc gacttcagac tctgcctcca gtacaactgg 1741 cccacaagta ttttgagctg tttcctgatc agaaagaccc tctctggcag aatccttgcg 1801 atgacaaacg ccacagagac atttggtcta aagaaaaaac ttgtgatcgc ttaccaaaat 1861 tcttggtaat aggaccccag aaaactggta ccactgcttt gtatttgttc ctggttatgc 1921 atccttccat ccttagtaac tcccccagcc caaaaacctt tgaggaggta cagttcttta 1981 atagaaataa ctaccacagg gggattgatt ggtatatgga tttcttccca gtcccatcta 2041 atgtcactac cgactttttg tttgagaaga gtgccaatta cttccactca gaggaagccc 2101 ctaaaagagc tgcttctctg gttcccaaag ccaagattat caccattctc attgaccctt 2161 cagaccgagc atactcctgg taccagcatc agcgatcaca tgaagaccct gcagctctga 2221 agtttagctt ctacgaagtg atctcagcag ggccccgtgc accctcggag ctcagagcct 2281 tgcagaagag atgtttggtc ccggggtggt atgccagcca catcgagaga tggcttgttt 2341 atttcccccc atttcagttg ctaattattg atgggcaaca actaagaact gatcctgcta 2401 cagtgatgga tgaagtacag aagtttctag gagtcttgcc tcattataat tactcagaag 2461 ctttaacgtt tgattctcat aaaggtttct ggtgtcagtt actggaagaa ggtaaaacaa 2521 aatgccttgg aaagagcaaa ggaagaaaat accctccaat ggattctgat agcaggacat 2581 ttctgtcaag ctactatcga gatcacaacg tggaactctc aaagctgctg cacaaactgg 2641 gtcagcctct gccatcctgg ctgagacagg agctgcagaa agtaagattg ccaactttct 2701 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2761 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta