Construct: ORF ccsbBroad304_07670
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003317.1_s304c1, BRDN0000462385
- Derived from:
- ccsbBroadEn_07670
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAG3 (10734)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07670
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282716.1 | 99.9% | 99.8% | 48G>T;106A>C;1293A>C |
| 2 | human | 10734 | STAG3 | stromal antigen 3 | NM_012447.4 | 99.9% | 99.8% | 48G>T;106A>C;1293A>C |
| 3 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282717.1 | 99.8% | 99.7% | (many diffs) |
| 4 | human | 10734 | STAG3 | stromal antigen 3 | XM_011515742.1 | 99.8% | 99.7% | (many diffs) |
| 5 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011686.2 | 98.7% | 98.6% | (many diffs) |
| 6 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011683.2 | 98.7% | 98.5% | (many diffs) |
| 7 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011684.1 | 98.7% | 98.5% | (many diffs) |
| 8 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011685.1 | 98.7% | 98.5% | (many diffs) |
| 9 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282718.2 | 95.1% | 95.1% | (many diffs) |
| 10 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011687.1 | 94.1% | 93.9% | (many diffs) |
| 11 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744740.2 | 23.3% | (many diffs) | |
| 12 | human | 378108 | TRIM74 | tripartite motif containing 74 | XM_017012197.2 | 13.1% | 11.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3744
- ORF length:
- 3675
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcttccccg ttgcaaagag ctgtgggaga taccaagagg gccttttctg 121 catcttctag ttcctctgcc agtctaccct ttgatgacag ggactcaaac catccctcag 181 aggggaatgg cgactctttg ttagctgatg aagacactga ctttgaagac agcttgaatc 241 gcaatgtgaa gaagagagca gcaaaacgac caccgaaaac aacaccggtg gcaaaacatc 301 caaagaaagg gtcccgagtg gtacatcgtc atagccggaa acagtcagag ccaccagcca 361 atgatctttt caatgctgtg aaagccgcca aaagtgacat gcagtctttg gtagatgagt 421 ggctggatag ctacaagcaa gaccaggatg caggatttct ggagcttgtt aactttttca 481 tccaatcttg cggatgtaaa ggcattgtga cccctgagat gttcaagaag atgtccaact 541 cagagatcat ccagcaccta acagagcagt ttaatgagga ctcgggggac taccctctca 601 tagctccagg tccatcctgg aagaagttcc agggcagctt ctgtgaattt gtgaggacat 661 tggtctgtca gtgccagtac agcctcctct atgatggctt ccctatggac gacctcatct 721 ccctgctcac tggcctctca gactcacaag tccgcgcctt ccgtcacact agcaccctgg 781 ctgctatgaa actgatgacc tccctggtaa aagttgccct ccaactgagt gtgcaccaag 841 ataacaatca gcgtcagtat gaggctgaaa gaaacaaggg gccagggcag agggcacctg 901 agcggctgga gagcctgttg gagaaacgca aagagctcca agagcatcaa gaggagattg 961 aggggatgat gaatgccctc ttcaggggtg tctttgttca tcggtacagg gatgtccttc 1021 ctgagatccg tgctatctgc attgaggaaa ttgggtgttg gatgcaaagc tacagcacgt 1081 ctttcctcac cgacagctat ttaaaatata ttggttggac tctgcatgat aagcaccgag 1141 aagtccgcct gaagtgtgtg aaggccctga aagggctgta cggtaaccgg gacctgacca 1201 cacgcctgga gctcttcacc agccgcttca aggaccggat ggtttccatg gtcatggaca 1261 gagagtatga tgtggcagtg gaggctgtca gattactgat acttatcctt aagaacatgg 1321 aaggggtgct gacggacgcg gattgtgaga gcgtctaccc cgttgtgtat gcctctcatc 1381 gaggcctggc ctctgccgca ggcgaatttc tgtactggaa actcttctac cctgagtgcg 1441 agataagaat gatgggtgga agagagcaac gccagagccc aggcgcccag aggactttct 1501 tccagcttct gctgtccttc tttgtggaga gcgagctcca tgaccacgct gcttacttag 1561 tagacagtct gtgggactgt gcaggggctc ggctgaagga ctgggagggt ctgacaagcc 1621 tgctgctgga gaaggaccag aacctgggtg atgtgcagga gagcacactg atagaaatcc 1681 ttgtgtccag tgcccggcaa gcttcagagg ggcacccgcc tgtgggccgg gtcactggga 1741 ggaagggctt aacctctaag gagcgcaaga cccaagccga tgacagggtg aagttgactg 1801 agcacctcat ccccctgctg ccccagctcc tggccaagtt ctcagctgat gcagagaagg 1861 tcactcccct gctccagctt ctcagctgct ttgacctcca catctactgc actgggcgct 1921 tggagaagca cctggagctg ttcctgcagc aactccagga ggtggtggtg aagcatgcag 1981 agccagcggt gcttgaggct ggggcgcatg ccctctacct gctctgtaat cccgaattca 2041 ctttcttcag ccgggcggac tttgcccgca gccagctagt agatttgctg actgaccgct 2101 tccagcagga gcttgaagag ctgttacagt cgtccttcct agatgaggat gaggtatata 2161 atctggcagc cactctgaaa cgcctctctg ccttctacaa cactcatgac ctgactcgct 2221 gggagctcta tgagccatgt tgccaactcc tgcagaaggc tgtggacaca ggagaggttc 2281 ctcaccaggt tatcctgcca gccttgactc ttgtctattt ttccattctc tggacactaa 2341 cccacatttc taaatcagat gcttcccaga agcagctgtc gagtttgagg gacagaatgg 2401 tggccttctg tgaactctgc cagagttgcc tctcagatgt ggatactgag atccaggagc 2461 aggcttttgt cttattaagt gatctacttc tcatctttag ccctcagatg attgttgggg 2521 gccgtgattt ccttaggcca cttgtctttt ttcctgaagc tactctccag tctgagctag 2581 ccagcttcct catggaccac gtcttcatcc agccgggaga cctgggcagt ggtgattccc 2641 aggaggatca tttacagata gagcggctac accagcggcg ccgcctccta gccgggttct 2701 gcaagctgtt gctttatggg gtgctggaga tggatgcagc ctcagatgtt ttcaaacact 2761 acaacaagtt ctacaatgac tatggtgaca ttatcaagga aacattaact agagcaaggc 2821 agattgaccg aagtcattgt tcccgaatcc tgctgctgag cctcaagcag ctgtacacag 2881 aactgctgca ggagcatggg ccccagggcc tgaatgagct tcctgccttc atcgagatga 2941 gggacctggc ccggaggttt gccttgagtt ttggacccca gcagctgcag aaccgtgacc 3001 tcgtggtcat gctacacaag gaaggcatcc agttctcctt gtctgagctt cctccagctg 3061 gctcctccaa tcagcctcca aatctggcat tcctggagct cctttcagag ttttcccccc 3121 gactcttcca tcaggacaag cagcttttac tgtcctatct agaaaagtgc ctgcagcatg 3181 tctcccaggc acctggccat ccctggggcc cagtcaccac ctactgccac tccctcagcc 3241 ctgtggagaa cacagcagag accagccctc aggtcctccc cagctccaag aggaggcgcg 3301 ttgaagggcc tgccaagcct aacagagagg acgtctcctc gtcccaggaa gaaagtctgc 3361 agctgaacag catcccgccc acgcccaccc tcacctccac agctgtgaag agcaggcagc 3421 ccctgtgggg gttgaaagag atggaggaag aagatggctc agagttggat tttgcccagg 3481 gtcagcccgt cgcaggcacc gagaggtcaa ggttcttggg tccacaatat ttccagactc 3541 cacacaaccc ttcaggtcct ggcctgggca accagctgat gcgactcagc cttatggaag 3601 aggacgagga agaagagtta gaaatccagg atgagtcaaa tgaagaacgg caggatacag 3661 acatgcaagc aagtagctac tcttccacca gtgagcgcgg gctggacctc ttagattcta 3721 cagagctgga tattgaggat ttcttgccaa ctttcttgta caaagtggtt ggtaagccta 3781 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 3841 gcgttaagtc gacaatcaac ctctggatta