Construct: ORF ccsbBroad304_07805

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017328.1_s304c1, BRDN0000411387
Derived from:
ccsbBroadEn_07805
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SCAF8 (22828)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07805
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22828 SCAF8 SR-related CTD associated f... NM_001286199.1 99.9% 99.9% 890A>T;3048C>T
2 human 22828 SCAF8 SR-related CTD associated f... NM_014892.4 99.9% 99.9% 890A>T;3048C>T
3 human 22828 SCAF8 SR-related CTD associated f... NM_001286194.1 96.5% 96.5% 1_135del;1025A>T;3183C>T
4 human 22828 SCAF8 SR-related CTD associated f... NM_001286189.1 95% 94.9% (many diffs)
5 human 22828 SCAF8 SR-related CTD associated f... NM_001286188.1 94.1% 94.1% 1_234del;1124A>T;3282C>T
6 mouse 106583 Scaf8 SR-related CTD-associated f... NM_134123.3 88.3% 91.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3879
ORF length:
3813
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga ggccgtgaag accttcaata gcgagttgta ttccctgaat gactataaac
121cacccatttc gaaagcgaaa atgacccaaa ttactaaggc agccatcaaa gctattaagt
181tctataaaca tgtggtacag agtgttgaga agtttattca gaaatgtaaa ccagaataca
241aagtacctgg actttatgtt attgactcca ttgtgcgaca atcccgacat cagtttggtc
301aagaaaagga tgtgtttgca cccagattta gtaataacat cattagcact ttccagaatt
361tatatcgttg ccctggggat gacaagagta aaatagtgag agtactaaac ttatggcaga
421agaataatgt atttaagagt gagattattc agcccctttt ggatatggca gccgggattc
481cgcctccagt tgtcacacct gttttggcca gcactaccac tgctatgagc aatactccag
541gaactcctgt gacacctgtt actccggcca atgtggtcca aggcttacct gatccgtggg
601tatctcagat aacaaataca gatacacttg cggctgtagc tcagatcttg caaagtcctc
661aaggccagca gcttcaacaa ttaatacaaa ccttacagat acaacaacag aagccccagc
721cttccattct gcaggcccta gatgctggtc ttgttgttca gttgcaagct cttacggcac
781aacttacagc tgcagctgca gctgccaaca ctcttactcc cttagaacag ggagtctcct
841ttaacaagaa gttgatggat aggtttgatt ttggggaaga ctctgagcat agtgaagaac
901ccaaaaagga aattccagct tcacaacttt ctcacgtttc agaatctgtg aacatttcca
961tttttcatca gatagcagaa caactacaac agcaaaacct agaacatctc agacagcagc
1021tcttggagca gcaacagcct caaaaggcca ctcctcagga tagtcaggaa ggaacctttg
1081ggtcagagca ttcagcgtca ccatcacaag ggagtagtca gcagcatttt cttgaacctg
1141aagtcaattt ggatgattcc atagatattc agcaacagga tatggatata gatgaagggc
1201aagatggagt ggaagaggag gtctttgaac aagaagctaa gaaagtggcg gttcgctcaa
1261gatcaagaac acattcacga tctcgttcaa gatcaccaag aaaacgaagg tctaggtcac
1321ggtctggctc tagaaagcgt aaacacagaa agcgatcacg ctcccgctca agagaaagaa
1381agaggaaatc atcacggtcg tattcaagtg aaaggagagc cagagaaagg gagaaagaac
1441gacagaaaaa gggattacct ccaattagat ctaaaacact aagtgtatgt agtactactc
1501tctgggttgg gcaagtggac aagaaggcaa cacagcaaga cttaaccaac ctgtttgaag
1561agtttggaca gattgaatcc attaatatga ttcctccccg gggctgtgct tatgtctgca
1621tggttcatcg acaagatgca tttcgagctc ttcagaaact cagttctgga tcatataaaa
1681ttgggtccaa ggtcattaag atcgcttggg ctttaaacaa aggtgtaaaa acagaataca
1741aacaattctg ggatgtggat cttggagtta catatatacc atgggaaaaa gttaaagtgg
1801atgacttgga aggttttgca gaaggaggca tgattgatca ggagactgta aatactgagt
1861gggaaactgt gaaaagctca gaacctgtta aagagacggt ccagacaact cagagcccaa
1921ctccagttga aaaggagaca gtggtcacaa cccaggcaga ggttttccct cctcctgttg
1981ctatgttgca gattccagtg gcgccagccg tgcctacagt tagtttagtc ccaccagcat
2041ttcctgtgtc gatgccggtt cctcctcctg gattcagtcc aatccctcca cctccttttt
2101taagagcaag ttttaaccct tcacaaccac cacctggttt catgccgcct ccagttcccc
2161cacctgttgt gccaccccct acgattccac cagtagtacc aacatcttta gtgcagccgt
2221cattatccat gacaccggaa actgtgaaag atgttggatt tggtagcctt gttataccag
2281gcggttctgt tgccagcaat cttgctactt ccgctctgcc agctggaaat gtttttaatg
2341ctccaactaa acaggcagag cctgaagaaa aagtacctca tcttatagac caccagattt
2401cttctggtga aaacaccaga tcagtgattc caaatgatat ttcaagtaat gctgcaattt
2461taggaggaca gccgccaaat gtgacaagca attctggaat tctgggagtc caaagaccaa
2521atgtatcaag taattctgaa attcttgggg tccggccatc taatgtttcc agtagttctg
2581ggattattgc agcccaacca ccaaatattc taaataactc tggaatattg ggaatacagc
2641cacccagtgt gtcaaatagt tctggacttt tgggagtgct acccccaaat atacctaaca
2701attctggact tgtaggagta cagccaccaa atgttccaaa tactcctgga cttctgggaa
2761cacagccacc agctggacct caaaacttac cccctttaag tatccctaat caaaggatgc
2821ccacaatgcc aatgttagac attcgtccgg gactaatacc acaggcacct gggccaagat
2881tccctttaat acagcctgga attccacccc aacggggaat cccaccccca tcggtacttg
2941attcagctct tcatccacca ccccgtggac cttttcctcc aggagatatt tttagtcaac
3001cagaaagacc ttttttagct cctggaagac aaagcgtaga caatgttact aacccagaaa
3061aaaggatacc acttgggaat gataacattc aacaggaagg agatagagat tatcggtttc
3121ctcctataga aaccagggaa agcattagta gacctccccc tgtggatgtt agagatgtgg
3181ttgggcggcc tatagatcca agagaaggtc ctggacggcc tccactagat ggtagggatc
3241attttggaag acctcctgta gatataagag agaatcttgt gaggccaggt atagatcatc
3301ttggtcgaag agaccacttt ggctttaatc cagagaagcc ctgggggcat agaggagatt
3361ttgatgagag agagcatcgg gttctaccgg tctatggtgg tccaaaaggc ttacatgaag
3421aaagaggtag atttcggtct ggaaactatc gatttgatcc tagaagtggt ccttggaacc
3481gaggatttgg acaagaagtt cacagagatt ttgatgaccg cagaagaccc tgggagaggc
3541aaagggatag ggatgacaga gattttgatt tctgcagaga aatgaatgga aatcgtcttg
3601gacgagacag aattcaaaac acttgggttc cccctcctca tgctcgggtt tttgattatt
3661ttgaaggggc cacttctcaa cgaaaaggtg ataatgtgcc tcaggttaat ggtgaaaata
3721cagagagaca tgctcagcca ccacctatac cagtacagaa tgatcctgaa ctttatgaaa
3781aactgacatc ttcaaatgaa ataaacaagg agaagagtga cacagttgct gatatagaaa
3841gtgaaccagt ggtagaaagc acagaaactg aggggacata cccaactttc ttgtacaaag
3901tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
3961gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)