Construct: ORF ccsbBroad304_08101
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007210.1_s304c1, BRDN0000402658
- Derived from:
- ccsbBroadEn_08101
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM230 (29058)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08101
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001009924.1 | 99.7% | 99.1% | 356A>C |
| 2 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001009925.1 | 99.7% | 99.1% | 356A>C |
| 3 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001330984.1 | 99.7% | 99.1% | 356A>C |
| 4 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001330985.1 | 99.7% | 99.1% | 356A>C |
| 5 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001330986.1 | 99.7% | 99.1% | 356A>C |
| 6 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_014145.4 | 99.7% | 99.1% | 356A>C |
| 7 | human | 29058 | TMEM230 | transmembrane protein 230 | XM_017027836.2 | 99.7% | 99.1% | 356A>C |
| 8 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001330987.1 | 66.4% | 56.9% | (many diffs) |
| 9 | human | 29058 | TMEM230 | transmembrane protein 230 | NM_001009923.2 | 65.3% | 65% | 1_189del;545A>C |
| 10 | human | 29058 | TMEM230 | transmembrane protein 230 | XM_024451879.1 | 46.3% | 40.1% | (many diffs) |
| 11 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958485.1 | 26% | (many diffs) | |
| 12 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958479.1 | 25.6% | (many diffs) | |
| 13 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958476.1 | 23.6% | (many diffs) | |
| 14 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958484.1 | 23.1% | (many diffs) | |
| 15 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958482.1 | 22.3% | (many diffs) | |
| 16 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958481.1 | 21.9% | (many diffs) | |
| 17 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958483.1 | 21.7% | (many diffs) | |
| 18 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958478.1 | 14.7% | (many diffs) | |
| 19 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958480.1 | 14.3% | (many diffs) | |
| 20 | human | 29058 | TMEM230 | transmembrane protein 230 | XR_002958477.1 | 12.8% | (many diffs) | |
| 21 | mouse | 70612 | Tmem230 | transmembrane protein 230 | NM_001141971.1 | 90.8% | 95.8% | (many diffs) |
| 22 | mouse | 70612 | Tmem230 | transmembrane protein 230 | NM_027478.3 | 90.8% | 95.8% | (many diffs) |
| 23 | mouse | 70612 | Tmem230 | transmembrane protein 230 | XM_006500166.3 | 90.8% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 426
- ORF length:
- 360
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat gccgtcccgt accaacctgg ctactggaat ccccagtagt aaagtgaaat 121 attcaaggct ctccagcaca gacgatggct acattgacct tcagtttaag aaaacccctc 181 ctaagatccc ttataaggcc atcgcacttg ccactgtgct gtttttgatt ggcgcctttc 241 tcattattat aggctccctc ctgctgtcag gctacatcag caaagggggg gcagaccggg 301 ccgttccagt gctgatcatt ggcattctgg tgttcctacc cggattttac cacctgcgca 361 tcgcttacta tgcatccaaa ggctaccgtg gttactccta tgatgacatt ccagactttg 421 ctgactaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 481 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 541 acctctggat ta