Construct: ORF ccsbBroad304_08255

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF003602.1_s304c1, BRDN0000404957
• Derived from: ccsbBroadEn_08255
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: ZNF219 (51222)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_08255
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	51222	ZNF219	zinc finger protein 219	NM_001101672.2	99.9%	99.8%	778C>A
2	human	51222	ZNF219	zinc finger protein 219	NM_001102454.1	99.9%	99.8%	778C>A
3	human	51222	ZNF219	zinc finger protein 219	NM_016423.3	99.9%	99.8%	778C>A
4	human	51222	ZNF219	zinc finger protein 219	XM_017021354.1	99.9%	99.8%	778C>A
5	human	51222	ZNF219	zinc finger protein 219	XM_011536825.1	95%	94.7%	1_97del;102_115del;889C>A
6	human	51222	ZNF219	zinc finger protein 219	XM_024449623.1	94.2%	94.1%	0_1ins123;655C>A
7	human	51222	ZNF219	zinc finger protein 219	XM_024449624.1	94.2%	94.1%	0_1ins123;655C>A
8	human	51222	ZNF219	zinc finger protein 219	XM_024449625.1	94.2%	94.1%	0_1ins123;655C>A
9	human	51222	ZNF219	zinc finger protein 219	XM_005267739.1	93.9%	93.6%	1_124del;129_142del;916C>A
10	mouse	69890	Zfp219	zinc finger protein 219	NM_001253694.1	82.4%	87.5%	(many diffs)
11	mouse	69890	Zfp219	zinc finger protein 219	NM_001253695.1	82.4%	87.5%	(many diffs)
12	mouse	69890	Zfp219	zinc finger protein 219	NM_027248.2	82.4%	87.5%	(many diffs)
13	mouse	69890	Zfp219	zinc finger protein 219	XM_006519498.3	82.4%	87.5%	(many diffs)
14	mouse	69890	Zfp219	zinc finger protein 219	XM_006519499.3	82.4%	87.5%	(many diffs)
15	mouse	69890	Zfp219	zinc finger protein 219	XM_006519501.3	82.4%	87.5%	(many diffs)
16	mouse	69890	Zfp219	zinc finger protein 219	XM_006519502.3	82.4%	87.5%	(many diffs)
17	mouse	69890	Zfp219	zinc finger protein 219	XM_006519503.2	82.4%	87.5%	(many diffs)
18	mouse	69890	Zfp219	zinc finger protein 219	XM_006519504.3	82.4%	87.5%	(many diffs)
19	mouse	69890	Zfp219	zinc finger protein 219	XM_006519505.1	82.4%	87.5%	(many diffs)
20	mouse	69890	Zfp219	zinc finger protein 219	XM_011245176.2	82.4%	87.5%	(many diffs)
21	mouse	69890	Zfp219	zinc finger protein 219	XM_011245177.1	82.4%	87.5%	(many diffs)
22	mouse	69890	Zfp219	zinc finger protein 219	XM_017316180.1	82.4%	87.5%	(many diffs)
23	mouse	69890	Zfp219	zinc finger protein 219	NM_001253696.1	78.2%	83.3%	(many diffs)
24	mouse	69890	Zfp219	zinc finger protein 219	XM_006519506.3	78.2%	83.3%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

2232

ORF length:

2166

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatgga gggctcacgt ccccgcgccc cgagcggcca cttagcgccg tcgccgccgg
121	ctttcgacgg cgagctggat ctgcagcgat actccaacgg gccagccgtg agcgcagggt
181	cgctcgggat gggagcggtg agctggtctg agagtcgtgc aggcgaacgg cgcttcccct
241	gccctgtatg cgggaagcgc ttccgcttca actctatcct tgctttgcac ctgcgggcgc
301	acccaggagc ccaggccttc cagtgccctc actgcggcca ccgcgcggcg cagcgggctc
361	tgctgcgctc gcacctgcgc acacaccagc ccgagcgccc acgtagtcct gctgcacgcc
421	tgttgctgga gttggaagag cgcgcgctac tacgcgaggc ccgactgggg agagcccgaa
481	gctcaggggg catgcaggcc acccctgcca ctgagggtct ggcgcggccc caggctcctt
541	catcgtccgc cttccgttgc ccctactgca aaggcaagtt tcgcacctcg gcggagcgcg
601	aacgccacct gcacatcctg cataggccct ggaagtgcgg cctgtgcagt ttcggctcca
661	gccaggagga ggagctgctg caccacagcc tgacggccca cggggctccc gagcgtcccc
721	tggcggccac ctccgctgcg cctccgcctc agcctcagcc tcagcctcca ccccagcccg
781	aacccagatc agtcccccag ccggagccgg agccggagcc cgaacgtgag gcaaccccga
841	ccacagctcc tgccgctccc gaggagcccc cagcgcctcc ggagttccgc tgccaagtgt
901	gcggccagag ctttacacag tcttggtttc tcaagggcca catgcgtaag cacaaggcct
961	ccttcgatca tgcgtgtccg gtgtgcggcc gctgcttcaa ggagccctgg ttccttaaga
1021	accacatgaa ggtgcacgcc agcaagctgg gcccactgcg tgccccgggg cctgcctccg
1081	ggcctgcccg cgccccccag cctcctgacc tcggcctgct ggcctatgag ccgttgggcc
1141	cagcgctcct cttggccccg gctcccaccc cggccgagcg ccgtgagccc ccgagcctct
1201	tgggctacct gagcctgcga gctggcgagg gccggcccaa cggcgagggt gctgagcccg
1261	gtcccggccg cagcttcgga ggcttccgcc cgctgtcctc tgctctcccg gcccgggctc
1321	gccggcaccg tgcggaggag cctgaggaag aagaggaggt ggtggaggcc gaggaggaaa
1381	cctgggcccg gggcaggtcg ctgggctctc tggcttccct gcacccgcgc ccgggtgagg
1441	gaccggggca ctctgcctct gctgctgggg cccaggcaag atcgaccgcc acgcaggaag
1501	agaatgggct gttggttgga gggacccggc ctgaaggggg ccggggcgcc accggcaagg
1561	attgtccttt ctgcggaaaa tctttccgct cagcacatca cctcaaagtg catctgcgag
1621	tgcacacagg cgagcggccc tacaagtgtc cgcactgcga ctacgcgggc acccagtccg
1681	gctcgctcaa gtatcaccta cagcgccacc accgggagca gaggagcggg gccggccccg
1741	ggccaccccc ggagccaccg cctccttccc agcggggttc ggccccgcaa tctggagcca
1801	agccgtctcc gcagcctgcg acctgggtgg agggcgcctc aagtccccgg cctccttcta
1861	gcggtgctgg gccggggtcc cgtcggaagc ccgccagccc tgggaggacc ctgcgcaacg
1921	ggcgaggcgg tgaggccgaa cccctggacc tgtccttgcg ggcagggccg ggaggcgagg
1981	ccgggcctgg gggtgccctc caccgctgcc tcttctgccc gttcgccact ggagccccag
2041	agctcatggc cttgcacctt caagtgcacc acagccgccg ggctaggggc cgccggccac
2101	cccaggctga cgcgtccccg ccctatgccc gagtaccatc aggagagacc cctcccagtc
2161	cttcgcagga aggggaggag ggctccgggc tgtccagacc cggagaggca gggctggggg
2221	ggcaagaacg gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
2281	tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
2341	caatcaacct ctggatta

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