Transcript: Human XM_017021354.1

PREDICTED: Homo sapiens zinc finger protein 219 (ZNF219), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF219 (51222)
Length:
2866
CDS:
238..2406

Additional Resources:

NCBI RefSeq record:
XM_017021354.1
NBCI Gene record:
ZNF219 (51222)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017021354.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000353582 CGCTTCCGCTTCAACTCTATC pLKO_005 430 CDS 100% 10.800 15.120 N ZNF219 n/a
2 TRCN0000369361 CACAAGGCCTCCTTCGATCAT pLKO_005 1123 CDS 100% 4.950 6.930 N ZNF219 n/a
3 TRCN0000016148 GCCAGAGCTTTACACAGTCTT pLKO.1 1076 CDS 100% 4.950 6.930 N ZNF219 n/a
4 TRCN0000329983 GCCAGAGCTTTACACAGTCTT pLKO_005 1076 CDS 100% 4.950 6.930 N ZNF219 n/a
5 TRCN0000016151 GCTTCAACTCTATCCTTGCTT pLKO.1 437 CDS 100% 3.000 4.200 N ZNF219 n/a
6 TRCN0000377280 CGATTAGCTTAGTGAGCTTAC pLKO_005 2421 3UTR 100% 6.000 4.800 N ZNF219 n/a
7 TRCN0000016149 CCCTACTGCAAAGGCAAGTTT pLKO.1 733 CDS 100% 5.625 4.500 N ZNF219 n/a
8 TRCN0000353583 CCATAGGCGTGAGAGTTTATT pLKO_005 2703 3UTR 100% 15.000 10.500 N ZNF219 n/a
9 TRCN0000329984 GCTCAGCACATCACCTCAAAG pLKO_005 1760 CDS 100% 10.800 7.560 N ZNF219 n/a
10 TRCN0000016152 GAAGAGAATGGGCTGTTGGTT pLKO.1 1669 CDS 100% 3.000 2.100 N ZNF219 n/a
11 TRCN0000016150 GCACATCACCTCAAAGTGCAT pLKO.1 1765 CDS 100% 2.640 1.584 N ZNF219 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017021354.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08255 pDONR223 100% 99.9% 99.8% None 778C>A n/a
2 ccsbBroad304_08255 pLX_304 0% 99.9% 99.8% V5 778C>A n/a
3 TRCN0000472416 ACTACCACGATGTTCCTCGAGGGT pLX_317 15.1% 99.9% 99.8% V5 778C>A n/a
4 ccsbBroadEn_15832 pDONR223 0% 99.6% 99.7% None 681_686delGCCTCA;729C>T n/a
5 ccsbBroad304_15832 pLX_304 0% 99.6% 99.7% V5 681_686delGCCTCA;729C>T n/a
6 TRCN0000475181 TTATCTCTCAACAGCCTCAATAGC pLX_317 17.7% 99.6% 99.7% V5 681_686delGCCTCA;729C>T n/a
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