Construct: ORF ccsbBroad304_08333
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005839.1_s304c1, BRDN0000402820
- Derived from:
- ccsbBroadEn_08333
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL48 (51642)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08333
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51642 | MRPL48 | mitochondrial ribosomal pro... | NM_016055.6 | 99.6% | 99% | 17A>G;617A>C |
| 2 | human | 51642 | MRPL48 | mitochondrial ribosomal pro... | XM_017017919.1 | 88.4% | 76.6% | (many diffs) |
| 3 | human | 51642 | MRPL48 | mitochondrial ribosomal pro... | NM_001318498.2 | 86.9% | 84.4% | (many diffs) |
| 4 | human | 51642 | MRPL48 | mitochondrial ribosomal pro... | NM_001318499.2 | 83.5% | 83% | 17A>G;111_233del;740A>C |
| 5 | human | 51642 | MRPL48 | mitochondrial ribosomal pro... | NM_001318500.2 | 56.7% | 56.1% | 17A>G;199_200ins273;344A>C |
| 6 | human | 51642 | MRPL48 | mitochondrial ribosomal pro... | NR_134658.2 | 39.7% | (many diffs) | |
| 7 | human | 51642 | MRPL48 | mitochondrial ribosomal pro... | NR_134659.2 | 35.8% | (many diffs) | |
| 8 | human | 51642 | MRPL48 | mitochondrial ribosomal pro... | NR_134660.2 | 35.6% | (many diffs) | |
| 9 | mouse | 52443 | Mrpl48 | mitochondrial ribosomal pro... | XM_006508021.3 | 46% | 45.7% | (many diffs) |
| 10 | mouse | 52443 | Mrpl48 | mitochondrial ribosomal pro... | XM_017322353.1 | 46% | 45.7% | (many diffs) |
| 11 | mouse | 52443 | Mrpl48 | mitochondrial ribosomal pro... | XM_017322355.1 | 46% | 45.7% | (many diffs) |
| 12 | mouse | 52443 | Mrpl48 | mitochondrial ribosomal pro... | XM_017322356.1 | 46% | 45.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 702
- ORF length:
- 636
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cggaaccttg ggaaaggtgc tgtgcctgag gaacaatacc atttttaagc 121 aagccttttc tctcttaagg tttagaactt caggagagaa gcccatctat tctgtaggtg 181 gaattctact aagtatcagt cggccctaca agacaaagcc cacccacggc attggaaagt 241 acaagcactt aattaaagca gaagagccca agaagaagaa gggaaaagtg gaagtgagag 301 ccattaattt ggggacagat tatgaatatg gggttttaaa tattcatctg actgcatatg 361 atatgaccct ggcagagagt tatgcccagt atgttcacaa cctctgcaac tctctctcca 421 ttaaagtcga ggaaagttat gcaatgccaa ccaaaaccat agaagtgttg cagttgcagg 481 accaaggcag caaaatgctc ctggactcag tgcttaccac ccatgagcga gtggttcaga 541 tcagcggttt gagtgctacg tttgcagaaa ttttcttgga aataatccaa agcagtcttc 601 ctgaaggagt cagactgtca gtgaaggagc acactgaaga agacttcaag ggacgattca 661 aagctcgacc agaactggaa gcactgttgg ccaagttgaa gtacccaact ttcttgtaca 721 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 781 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta