Transcript: Human NR_134659.2

Homo sapiens mitochondrial ribosomal protein L48 (MRPL48), transcript variant 6, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
MRPL48 (51642)
Length:
1753
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_134659.2
NBCI Gene record:
MRPL48 (51642)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_134659.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000240819 ACTTCAAGGGACGATTCAAAG pLKO_005 929 3UTR 100% 10.800 8.640 N MRPL48 n/a
2 TRCN0000240817 GCCTGAGGAACAATACCATTT pLKO_005 380 3UTR 100% 10.800 8.640 N MRPL48 n/a
3 TRCN0000180011 CCACGGCATTGGAAAGTACAA pLKO.1 510 3UTR 100% 4.950 3.960 N MRPL48 n/a
4 TRCN0000240821 GTGGAAGTGAGAGCCATTAAT pLKO_005 574 3UTR 100% 15.000 10.500 N MRPL48 n/a
5 TRCN0000240818 GCAACTCTCTCTCCATTAAAG pLKO_005 692 3UTR 100% 13.200 9.240 N MRPL48 n/a
6 TRCN0000179227 GAAGAGCTTACTGGGTAGTTA pLKO.1 1040 3UTR 100% 5.625 3.938 N MRPL48 n/a
7 TRCN0000180879 GCAGAGAGTTATGCCCAGTAT pLKO.1 658 3UTR 100% 4.950 3.465 N MRPL48 n/a
8 TRCN0000240820 CATGCCAGCAGTGGTCATATT pLKO_005 1007 3UTR 100% 13.200 7.920 N MRPL48 n/a
9 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1668 3UTR 100% 5.625 2.813 Y KLHL30 n/a
10 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1668 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_134659.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08333 pDONR223 100% 35.8% None (many diffs) n/a
2 ccsbBroad304_08333 pLX_304 0% 35.8% V5 (many diffs) n/a
3 TRCN0000470218 ACGCCGTTTGAACGGCTAGCTGTA pLX_317 77.5% 35.8% V5 (many diffs) n/a
4 ccsbBroadEn_13781 pDONR223 100% 13.8% None (many diffs) n/a
5 ccsbBroad304_13781 pLX_304 0% 13.8% V5 (many diffs) n/a
6 TRCN0000469746 TCCCTGCGCCGTCCGGGTTTTCGA pLX_317 100% 13.8% V5 (many diffs) n/a
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