Construct: ORF ccsbBroad304_08575

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002103.1_s304c1, BRDN0000405664
Derived from:
ccsbBroadEn_08575
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EDEM2 (55741)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08575
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55741 EDEM2 ER degradation enhancing al... NM_018217.3 99.9% 99.8% 1366G>A
2 human 55741 EDEM2 ER degradation enhancing al... NM_001145025.2 93.5% 93.4% 104_105ins111;1255G>A
3 human 111089941 MMP24-AS1-EDEM2 MMP24-AS1-EDEM2 readthrough NM_001355008.2 92.8% 92.7% 0_1ins123;1243G>A
4 human 55741 EDEM2 ER degradation enhancing al... NR_026728.2 82.5% (many diffs)
5 mouse 108687 Edem2 ER degradation enhancer, ma... NM_145537.2 88.5% 93.6% (many diffs)
6 mouse 108687 Edem2 ER degradation enhancer, ma... XM_006498555.1 45.5% 47.1% (many diffs)
7 mouse 108687 Edem2 ER degradation enhancer, ma... XR_866205.2 42% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1800
ORF length:
1734
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc tttccggctg ctcatcccgc tcggcctcct gtgcgcgctg ctgcctcagc
121accatggtgc gccaggtccc gacggctccg cgccagatcc cgcccactac agggagcgag
181tcaaggccat gttctaccac gcctacgaca gctacctgga gaatgccttt cccttcgatg
241agctgcgacc tctcacctgt gacgggcacg acacctgggg cagtttttct ctgactctaa
301ttgatgcact ggacaccttg ctgattttgg ggaatgtctc agaattccaa agagtggttg
361aagtgctcca ggacagcgtg gactttgata ttgatgtgaa cgcctctgtg tttgaaacaa
421acattcgagt ggtaggagga ctcctgtctg ctcatctgct ctccaagaag gctggggtgg
481aagtagaggc tggatggccc tgttccgggc ctctcctgag aatggctgag gaggcggccc
541gaaaactcct cccagccttt cagaccccca ctggcatgcc atatggaaca gtgaacttac
601ttcatggcgt gaacccagga gagacccctg tcacctgtac ggcagggatt gggaccttca
661ttgttgaatt tgccaccctg agcagcctca ctggtgaccc ggtgttcgaa gatgtggcca
721gagtggcttt gatgcgcctc tgggagagcc ggtcagatat cgggctggtc ggcaaccaca
781ttgatgtgct cactggcaag tgggtggccc aggacgcagg catcggggct ggcgtggact
841cctactttga gtacttggtg aaaggagcca tcctgcttca ggataagaag ctcatggcca
901tgttcctaga gtataacaaa gccatccgga actacacccg cttcgatgac tggtacctgt
961gggttcagat gtacaagggg actgtgtcca tgccagtctt ccagtccttg gaggcctact
1021ggcctggtct tcagagcctc attggagaca ttgacaatgc catgaggacc ttcctcaact
1081actacactgt atggaagcag tttggggggc tcccggaatt ctacaacatt cctcagggat
1141acacagtgga gaagcgagag ggctacccac ttcggccaga acttattgaa agcgcaatgt
1201acctctaccg tgccacgggg gatcccaccc tcctagaact cggaagagat gctgtggaat
1261ccattgaaaa aatcagcaag gtggagtgcg gatttgcaac aatcaaagat ctgcgagacc
1321acaagctgga caaccgcatg gagtcgttct tcctggccga gactgtgaaa tacctctacc
1381tcctgtttga cccaaccaac ttcatccaca acaatgggtc caccttcgac acggtgatca
1441ccccctatgg ggagtgcatc ctgggggctg gggggtacat cttcaacaca gaagctcacc
1501ccatcgaccc tgccgccctg cactgctgcc agaggctgaa ggaagagcag tgggaggtgg
1561aggacttgat gagggaattc tactctctca aacggagcag gtcgaaattt cagaaaaaca
1621ctgttagttc ggggccatgg gaacctccag caaggccagg aacactcttc tcaccagaaa
1681accatgacca ggcaagggag aggaagcctg ccaaacagaa ggtcccactt ctcagctgcc
1741ccagtcagcc cttcacctcc aagttggcat tactgggaca ggttttccta gactcctcat
1801acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1861attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1921ggatta

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