Construct: ORF ccsbBroad304_08603

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016659.1_s304c1, BRDN0000404165
Derived from:
ccsbBroadEn_08603
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CBWD1 (55871)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08603
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55871 CBWD1 COBW domain containing 1 NM_001145355.1 99.9% 100% 468A>G
2 human 150472 CBWD2 COBW domain containing 2 NM_001330339.1 99% 98.6% (many diffs)
3 human 55871 CBWD1 COBW domain containing 1 XM_011517961.2 94.6% 94.7% 468A>G;596_597ins57
4 human 55871 CBWD1 COBW domain containing 1 NM_018491.3 88.7% 86.7% (many diffs)
5 human 150472 CBWD2 COBW domain containing 2 NM_172003.3 88% 85.5% (many diffs)
6 human 445571 CBWD3 COBW domain containing 3 NM_201453.3 87.9% 85% (many diffs)
7 human 220869 CBWD5 COBW domain containing 5 NM_001330668.1 87.9% 84.7% (many diffs)
8 human 644019 CBWD6 COBW domain containing 6 NM_001085457.1 87.5% 84.2% (many diffs)
9 human 55871 CBWD1 COBW domain containing 1 NM_001145356.1 84% 82% (many diffs)
10 human 55871 CBWD1 COBW domain containing 1 XM_005251513.4 83.7% 81.7% (many diffs)
11 human 150472 CBWD2 COBW domain containing 2 NM_001330336.1 83.2% 80.7% (many diffs)
12 human 445571 CBWD3 COBW domain containing 3 XM_005277637.2 83.1% 80.2% (many diffs)
13 human 220869 CBWD5 COBW domain containing 5 NM_001363751.1 83.1% 80% (many diffs)
14 human 220869 CBWD5 COBW domain containing 5 XM_005272750.1 83% 80% (many diffs)
15 human 445571 CBWD3 COBW domain containing 3 NM_001291821.1 82.9% 80% (many diffs)
16 human 644019 CBWD6 COBW domain containing 6 XM_017015035.1 82.7% 79.5% (many diffs)
17 human 644019 CBWD6 COBW domain containing 6 XM_017015020.1 82.6% 79.2% (many diffs)
18 human 55871 CBWD1 COBW domain containing 1 XM_005251514.1 81.5% 79.5% (many diffs)
19 human 150472 CBWD2 COBW domain containing 2 NM_001330337.1 80.9% 78.5% (many diffs)
20 human 445571 CBWD3 COBW domain containing 3 XM_005277639.2 80.9% 78% (many diffs)
21 human 220869 CBWD5 COBW domain containing 5 XM_005272751.1 80.9% 77.7% (many diffs)
22 human 644019 CBWD6 COBW domain containing 6 XM_024447653.1 80.6% 77.5% (many diffs)
23 human 644019 CBWD6 COBW domain containing 6 XM_017015019.1 79.6% 76.3% (many diffs)
24 human 644019 CBWD6 COBW domain containing 6 XM_017015023.1 77.8% 74.5% (many diffs)
25 human 55871 CBWD1 COBW domain containing 1 XM_005251515.1 76.8% 74.7% (many diffs)
26 human 150472 CBWD2 COBW domain containing 2 XM_005263604.2 76.1% 73.7% (many diffs)
27 human 445571 CBWD3 COBW domain containing 3 XM_005277640.2 76.1% 73.2% (many diffs)
28 human 220869 CBWD5 COBW domain containing 5 NM_001024916.3 76.1% 73% (many diffs)
29 human 445571 CBWD3 COBW domain containing 3 XM_017014730.1 75.9% 73% (many diffs)
30 human 644019 CBWD6 COBW domain containing 6 XM_017015036.1 75.8% 72.7% (many diffs)
31 human 644019 CBWD6 COBW domain containing 6 XM_017015024.1 75.7% 72.5% (many diffs)
32 human 644019 CBWD6 COBW domain containing 6 XM_017015021.1 75% 71.8% (many diffs)
33 human 220869 CBWD5 COBW domain containing 5 XM_017014456.1 74.9% 74% (many diffs)
34 human 220869 CBWD5 COBW domain containing 5 XM_024447453.1 74.9% 74% (many diffs)
35 human 644019 CBWD6 COBW domain containing 6 XM_017015022.1 73% 69.8% (many diffs)
36 human 150472 CBWD2 COBW domain containing 2 XM_024452717.1 69.6% 69.3% (many diffs)
37 human 55871 CBWD1 COBW domain containing 1 XM_011517962.3 69.2% 67.2% (many diffs)
38 human 55871 CBWD1 COBW domain containing 1 XM_011517963.1 68.7% 68.8% 0_1ins336;132A>G
39 human 150472 CBWD2 COBW domain containing 2 XM_011510667.1 68.6% 66.5% (many diffs)
40 human 445571 CBWD3 COBW domain containing 3 XM_006717116.1 68.6% 66.2% (many diffs)
41 human 220869 CBWD5 COBW domain containing 5 XM_006716914.1 68.6% 66% (many diffs)
42 human 644019 CBWD6 COBW domain containing 6 XM_017015025.1 68.4% 65.3% (many diffs)
43 human 220869 CBWD5 COBW domain containing 5 XM_017014457.1 67.1% 66.2% (many diffs)
44 human 644019 CBWD6 COBW domain containing 6 XM_017015028.1 66.3% 65.2% (many diffs)
45 human 644019 CBWD6 COBW domain containing 6 XM_017015029.2 64.8% 64.1% (many diffs)
46 human 445571 CBWD3 COBW domain containing 3 XR_001746301.1 63.6% (many diffs)
47 human 220869 CBWD5 COBW domain containing 5 XM_017014455.1 61.6% 59% (many diffs)
48 human 644019 CBWD6 COBW domain containing 6 XM_017015026.1 61.2% 58.5% (many diffs)
49 human 445571 CBWD3 COBW domain containing 3 XR_001746303.1 60.8% (many diffs)
50 human 150472 CBWD2 COBW domain containing 2 NM_001330340.1 60.5% 59.1% (many diffs)
51 human 150472 CBWD2 COBW domain containing 2 NM_001330342.1 60.5% 59.1% (many diffs)
52 human 220869 CBWD5 COBW domain containing 5 XM_017014459.1 60.4% 59.1% (many diffs)
53 human 445571 CBWD3 COBW domain containing 3 XM_011518706.2 60.3% 59.1% (many diffs)
54 human 644019 CBWD6 COBW domain containing 6 XM_024447652.1 60% 58.3% (many diffs)
55 human 445571 CBWD3 COBW domain containing 3 XR_001746302.1 58.6% (many diffs)
56 human 220869 CBWD5 COBW domain containing 5 XR_001746234.1 58% (many diffs)
57 human 220869 CBWD5 COBW domain containing 5 XR_428504.1 57.9% (many diffs)
58 human 644019 CBWD6 COBW domain containing 6 XM_017015031.1 57.6% 56% (many diffs)
59 human 55871 CBWD1 COBW domain containing 1 XR_001746352.1 57.2% (many diffs)
60 human 644019 CBWD6 COBW domain containing 6 XM_017015027.1 56.7% 49.6% (many diffs)
61 human 220869 CBWD5 COBW domain containing 5 XR_001746232.1 56.6% (many diffs)
62 human 445571 CBWD3 COBW domain containing 3 XR_001746299.1 55.9% (many diffs)
63 human 445571 CBWD3 COBW domain containing 3 XR_001746297.1 54.9% (many diffs)
64 human 220869 CBWD5 COBW domain containing 5 XR_002956769.1 54.7% (many diffs)
65 human 150472 CBWD2 COBW domain containing 2 NR_138473.1 54.7% (many diffs)
66 human 220869 CBWD5 COBW domain containing 5 XR_001746237.2 53.2% (many diffs)
67 human 150472 CBWD2 COBW domain containing 2 NR_138474.1 53.1% (many diffs)
68 human 220869 CBWD5 COBW domain containing 5 XM_017014461.1 52.7% 51.5% (many diffs)
69 human 445571 CBWD3 COBW domain containing 3 XM_017014732.1 52.6% 51.5% (many diffs)
70 human 445571 CBWD3 COBW domain containing 3 XR_001746300.1 52.5% (many diffs)
71 human 644019 CBWD6 COBW domain containing 6 XM_017015030.1 52% 44.9% (many diffs)
72 human 644019 CBWD6 COBW domain containing 6 XR_001746360.1 51.6% (many diffs)
73 human 220869 CBWD5 COBW domain containing 5 XR_002956768.1 50.7% (many diffs)
74 human 644019 CBWD6 COBW domain containing 6 XM_017015033.1 50.3% 48.9% (many diffs)
75 human 55871 CBWD1 COBW domain containing 1 XM_005251517.5 49.7% 46.8% (many diffs)
76 human 445571 CBWD3 COBW domain containing 3 XR_002956784.1 49% (many diffs)
77 human 220869 CBWD5 COBW domain containing 5 XR_428503.2 49% (many diffs)
78 human 55871 CBWD1 COBW domain containing 1 XM_017014925.1 48.6% 47% (many diffs)
79 human 220869 CBWD5 COBW domain containing 5 XM_017014458.1 48% 45.7% (many diffs)
80 human 220869 CBWD5 COBW domain containing 5 NM_001286835.1 47.9% 42.9% (many diffs)
81 human 445571 CBWD3 COBW domain containing 3 XR_929802.1 45.7% (many diffs)
82 human 644019 CBWD6 COBW domain containing 6 XM_017015032.1 44.1% 40.6% (many diffs)
83 human 150472 CBWD2 COBW domain containing 2 XR_244814.2 44% (many diffs)
84 human 220869 CBWD5 COBW domain containing 5 XR_001746239.1 42.7% (many diffs)
85 human 55871 CBWD1 COBW domain containing 1 XM_011517964.3 42.5% 39.6% (many diffs)
86 human 445571 CBWD3 COBW domain containing 3 XR_929803.1 42.1% (many diffs)
87 human 220869 CBWD5 COBW domain containing 5 XR_001746240.1 41.5% (many diffs)
88 human 644019 CBWD6 COBW domain containing 6 XR_001746359.1 41.4% (many diffs)
89 human 55871 CBWD1 COBW domain containing 1 XR_001746353.1 40.7% (many diffs)
90 human 220869 CBWD5 COBW domain containing 5 XR_001746238.1 40.1% (many diffs)
91 human 445571 CBWD3 COBW domain containing 3 XR_929804.1 39.8% (many diffs)
92 human 150472 CBWD2 COBW domain containing 2 XR_922864.1 39.4% (many diffs)
93 human 445571 CBWD3 COBW domain containing 3 XR_001746298.1 38.8% (many diffs)
94 human 445571 CBWD3 COBW domain containing 3 XR_001746304.1 38.5% (many diffs)
95 human 150472 CBWD2 COBW domain containing 2 XR_427073.2 38.3% (many diffs)
96 human 644019 CBWD6 COBW domain containing 6 XR_001746361.1 38.2% (many diffs)
97 human 220869 CBWD5 COBW domain containing 5 XR_001746241.2 36.8% (many diffs)
98 human 644019 CBWD6 COBW domain containing 6 XR_001746362.1 35.8% (many diffs)
99 human 644019 CBWD6 COBW domain containing 6 XM_017015034.1 35% 32.8% (many diffs)
100 human 644019 CBWD6 COBW domain containing 6 XR_001746363.1 34.8% (many diffs)
101 human 150472 CBWD2 COBW domain containing 2 XR_002959242.1 34.2% (many diffs)
102 human 220869 CBWD5 COBW domain containing 5 NR_104600.1 24.4% (many diffs)
103 human 220869 CBWD5 COBW domain containing 5 XR_002956766.1 23.4% (many diffs)
104 human 220869 CBWD5 COBW domain containing 5 XR_001746233.1 23.2% (many diffs)
105 human 220869 CBWD5 COBW domain containing 5 XR_001746235.1 23% (many diffs)
106 human 220869 CBWD5 COBW domain containing 5 XR_001746231.1 22.8% (many diffs)
107 human 220869 CBWD5 COBW domain containing 5 XR_002956767.1 20.2% (many diffs)
108 human 220869 CBWD5 COBW domain containing 5 NM_001286836.1 17.9% 16% (many diffs)
109 mouse 226043 Cbwd1 COBW domain containing 1 NM_146097.3 75.9% 73.1% (many diffs)
110 mouse 226043 Cbwd1 COBW domain containing 1 NR_033744.1 45.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1143
ORF length:
1077
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgta tttcaaacgt gccgctcggg cctttcccgt attgctcact ggtgctggga
121agacaacact tctgaactat attttgacag agcaacatag taaaagagta gcggtcattt
181taaatgaatt tggggaagga agtgcgctgg agaaatcctt agctgtcagc caaggtggag
241agctctatga agagtggctg gaacttagaa acggttgcct ctgctgttca gtgaaggaca
301gtggccttag agctattgag aatttgatgc aaaagaaggg gaaatttgat tacatactgt
361tagagaccac tggattagca gaccctggtg cagtggcttc tatgttttgg gttgatgctg
421aattagggag tgatatttat cttgatggta tcataactat tgtggattca aaatatggat
481taaaacattt aacagaagag aaacctgatg gccttatcaa tgaagctact aggcaagttg
541ctttggcaga tgccattctc attaataaaa cagacctggt tccagaagaa gatgtaaaga
601aattaagaac gacaattaga tccataaatg gactaggaca aatcttagaa acacaaagat
661caagagttga tctctctaat gtattagatc ttcatgcctt tgatagtctc tctggaataa
721gtttgcagaa aaaacttcag catgtgccag gaacacaacc tcaccttgat cagagtattg
781ttacaatcac atttgaagta ccaggaaatg caaaggaaga acatcttaat atgtttattc
841agaatctcct gtgggaaaag aatgtgagaa acaaggacaa tcactgcatg gaggtcataa
901ggctgaaggg attggtgtca atcaaagaca aatcacaaca agtgattgtc cagggtgtcc
961atgagctcta tgatctggag gagactccag tgagctggaa ggatgacact gagagaacaa
1021atcgattggt cctccttggc agaaatttag ataaggatat ccttaaacag ctgtttatag
1081ctactgtgac agaaacagaa aagcagtgga caacacgttt ccaagaagat caagtttgta
1141catacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
1201tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
1261tctggatta

Download FASTA (ORF) (Full)