Construct: ORF ccsbBroad304_09483

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015237.1_s304c1, BRDN0000406454
Derived from:
ccsbBroadEn_09483
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MSI2 (124540)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09483
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 124540 MSI2 musashi RNA binding protein 2 NM_138962.4 99.7% 99.6% 6G>N;321A>G
2 human 124540 MSI2 musashi RNA binding protein 2 XM_005257014.3 94.6% 94.5% 6G>N;321A>G;788_841del
3 human 124540 MSI2 musashi RNA binding protein 2 XM_005257015.3 91.1% 89.7% (many diffs)
4 human 124540 MSI2 musashi RNA binding protein 2 NM_001322250.1 88.3% 88.4% 0_1ins66;255A>G;722_775del
5 human 124540 MSI2 musashi RNA binding protein 2 XM_017024147.2 85.7% 85.5% 6G>N;308_309ins93;695_748del
6 human 124540 MSI2 musashi RNA binding protein 2 XM_017024148.1 82% 81% (many diffs)
7 human 124540 MSI2 musashi RNA binding protein 2 NM_001322251.2 73.7% 74.3% (many diffs)
8 human 124540 MSI2 musashi RNA binding protein 2 NM_170721.2 69% 69.8% (many diffs)
9 human 124540 MSI2 musashi RNA binding protein 2 XM_017024149.2 68.5% 65.7% (many diffs)
10 human 124540 MSI2 musashi RNA binding protein 2 XM_011524286.2 64.6% 64.7% 0_1ins312;9A>G;476_529del
11 human 124540 MSI2 musashi RNA binding protein 2 XM_011524287.3 59.4% 53.7% (many diffs)
12 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534442.3 95.3% 99.3% (many diffs)
13 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_017314830.1 93% 96.7% (many diffs)
14 mouse 76626 Msi2 musashi RNA-binding protein 2 NM_054043.3 90.3% 94.2% (many diffs)
15 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534440.2 88.8% 92.6% (many diffs)
16 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_011249302.1 88.3% 91.8% (many diffs)
17 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534439.2 86.8% 90.2% (many diffs)
18 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534435.2 84.5% 88.1% (many diffs)
19 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534438.2 83.7% 85.7% (many diffs)
20 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534432.2 82.7% 85.9% (many diffs)
21 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534434.2 81.5% 83.7% (many diffs)
22 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534431.2 79.8% 81.7% (many diffs)
23 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_006534441.3 75.3% 72.8% (many diffs)
24 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_011249304.2 69.7% 69.3% (many diffs)
25 mouse 76626 Msi2 musashi RNA-binding protein 2 XM_011249305.2 63.5% 61.9% (many diffs)
26 mouse 76626 Msi2 musashi RNA-binding protein 2 NM_001201341.1 43.2% 33.4% (many diffs)
27 mouse 76626 Msi2 musashi RNA-binding protein 2 XR_879764.1 14.2% (many diffs)
28 mouse 76626 Msi2 musashi RNA-binding protein 2 XR_388550.2 14.1% (many diffs)
29 mouse 76626 Msi2 musashi RNA-binding protein 2 XR_001780073.1 14% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1050
ORF length:
984
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga ngcaaatggg agccaaggca cctcgggcag cgccaacgac tcccagcacg
121accccggtaa aatgtttatc ggtggactga gctggcagac ctcaccagat agccttagag
181actattttag caaatttgga gaaattagag aatgtatggt catgagagat cccactacga
241aacgctccag aggcttcggt ttcgtcacgt tcgcagaccc agcaagtgta gataaagtat
301taggtcagcc ccaccatgag ttagattcca agacgattga ccccaaagtt gcatttcctc
361gtcgagcgca acccaagatg gtcacgagaa caaagaaaat atttgtaggc gggttatctg
421cgaacacagt agtggaagat gtaaagcaat atttcgagca gtttggcaag gtggaagatg
481caatgctgat gtttgataaa actaccaaca ggcacagagg gtttggcttt gtcacttttg
541agaatgaaga tgttgtggag aaagtctgtg agattcattt ccatgaaatc aataataaaa
601tggtagaatg taagaaagct cagccgaaag aagtcatgtt cccacctggg acaagaggcc
661gggcccgggg actgccttac accatggacg cgttcatgct tggcatgggg atgctgggat
721atcccaactt cgtggcgacc tatggccgtg gctaccccgg atttgctcca agctatggct
781atcagttccc aggcttccca gcagcggctt atggaccagt ggcagcagcg gcggtggcgg
841cagcaagagg atcaggctcc aacccggcgc ggcccggagg cttcccgggg gccaacagcc
901caggacctgt cgccgatctc tacggccctg ccagccagga ctccggagtg gggaattaca
961taagtgcggc cagcccacag ccgggctcgg gcttcggcca cggcatagct ggacctttga
1021ttgcaacggc ctttacaaat ggataccatt gcccaacttt cttgtacaaa gtggttggta
1081agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1141ggtggcgcgt taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)