Construct: ORF ccsbBroad304_09557
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017041.1_s304c1, BRDN0000405309
- Derived from:
- ccsbBroadEn_09557
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STXBP5 (134957)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09557
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 134957 | STXBP5 | syntaxin binding protein 5 | NM_139244.4 | 99.9% | 100% | 3285A>G |
| 2 | human | 134957 | STXBP5 | syntaxin binding protein 5 | XM_006715339.3 | 98.2% | 98.2% | 2146_2205del;3345A>G |
| 3 | human | 134957 | STXBP5 | syntaxin binding protein 5 | NM_001127715.2 | 96.8% | 96.8% | 2146_2253del;3393A>G |
| 4 | human | 134957 | STXBP5 | syntaxin binding protein 5 | XM_017010267.1 | 78.8% | 78.8% | (many diffs) |
| 5 | human | 134957 | STXBP5 | syntaxin binding protein 5 | XM_011535453.1 | 69.2% | 69.2% | 0_1ins987;1159_1218del;2358A>G |
| 6 | human | 134957 | STXBP5 | syntaxin binding protein 5 | XM_017010268.2 | 65.5% | 62.8% | (many diffs) |
| 7 | human | 134957 | STXBP5 | syntaxin binding protein 5 | XR_245504.2 | 64.8% | 1_349del;2495_2557del;2850_2851ins907 | |
| 8 | human | 134957 | STXBP5 | syntaxin binding protein 5 | XR_001743163.2 | 40.8% | (many diffs) | |
| 9 | human | 134957 | STXBP5 | syntaxin binding protein 5 | XR_002956261.1 | 38.8% | (many diffs) | |
| 10 | human | 134957 | STXBP5 | syntaxin binding protein 5 | XR_245502.3 | 34.8% | (many diffs) | |
| 11 | mouse | 78808 | Stxbp5 | syntaxin binding protein 5 ... | NM_001081344.2 | 88.8% | 97% | (many diffs) |
| 12 | mouse | 78808 | Stxbp5 | syntaxin binding protein 5 ... | XM_006512928.2 | 87.4% | 95.5% | (many diffs) |
| 13 | mouse | 78808 | Stxbp5 | syntaxin binding protein 5 ... | XM_006512927.3 | 87.3% | 95.3% | (many diffs) |
| 14 | mouse | 78808 | Stxbp5 | syntaxin binding protein 5 ... | XM_006512926.3 | 86.1% | 94% | (many diffs) |
| 15 | mouse | 78808 | Stxbp5 | syntaxin binding protein 5 ... | XM_011243236.2 | 60.2% | 62.7% | (many diffs) |
| 16 | mouse | 78808 | Stxbp5 | syntaxin binding protein 5 ... | XM_011243237.2 | 59.6% | 63.8% | (many diffs) |
| 17 | mouse | 78808 | Stxbp5 | syntaxin binding protein 5 ... | XM_011243238.2 | 59.2% | 63.3% | (many diffs) |
| 18 | mouse | 78808 | Stxbp5 | syntaxin binding protein 5 ... | XR_380096.3 | 51.3% | (many diffs) | |
| 19 | mouse | 78808 | Stxbp5 | syntaxin binding protein 5 ... | XR_380094.2 | 50.5% | (many diffs) | |
| 20 | mouse | 78808 | Stxbp5 | syntaxin binding protein 5 ... | XR_380092.2 | 49.9% | (many diffs) | |
| 21 | mouse | 78808 | Stxbp5 | syntaxin binding protein 5 ... | XR_001779636.1 | 39.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3414
- ORF length:
- 3345
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggaaattc aacatcagga aggtgctgga cggcctgacc gccggctcgt 121 cctcggcgtc gcagcagcaa cagcagcagc atccgcctgg gaaccgggag ccggagatcc 181 aggaaacgct ccagtccgag cactttcagc tctgcaagac tgttcgccat ggatttccct 241 atcaaccctc agccctggcc tttgatcctg tacagaagat cctggcagtg ggaactcaga 301 ctggtgcttt aaggctcttt ggtcgtccag gagtagaatg ttattgccag catgacagtg 361 gagctgcagt aatccagctc cagttcctga ttaatgaggg agcgcttgtg agtgccttgg 421 ctgatgacac cttacactta tggaatttac gtcagaagag gcctgccata ctacattcgc 481 ttaaattttg cagagaaagg gttacatttt gccatctgcc tttccagagt aagtggctct 541 atgtgggcac tgaacgaggt aatatacata ttgtcaatgt ggagtccttc acactctcag 601 gctacgtcat tatgtggaat aaagccattg aactgtcatc taaatctcac ccaggacctg 661 tggtccatat aagtgataat ccaatggacg agggaaagct tttgattggc tttgaatctg 721 gaacagtagt tttatgggac ctcaaatcaa agaaagccga ctacagatac acatatgatg 781 aggctatcca ctctgttgct tggcatcatg aaggaaaaca atttatttgc agtcattcag 841 atggcacctt gactatatgg aatgtaaggt cccctgctaa accagtacag acaatcactc 901 cacatggaaa acagttaaag gatgggaaga agccagaacc atgcaaacct atcctcaagg 961 tggaattcaa aacgactaga tctggggagc cttttattat tttatcagga ggtttgtcat 1021 atgatactgt aggaagaaga ccttgcttaa cagtgatgca tgggaaaagc actgctgtgc 1081 tagaaatgga ctattcaatt gttgattttc taacgctgtg tgaaacacca tacccaaatg 1141 attttcaaga accatatgct gtggttgttc ttctagaaaa ggatttagta cttatagacc 1201 ttgcacaaaa tggatatcct atatttgaaa atccctaccc tttgagtata catgagtccc 1261 ctgttacatg ttgcgaatat tttgcggatt gtcctgtgga ccttattcct gcactttatt 1321 ctgttggagc tagacagaaa cgtcaaggtt acagcaaaaa ggaatggccc atcaacggag 1381 gtaattgggg cttgggtgct caaagttacc cagaaataat tattacaggg catgctgatg 1441 ggtcagttaa gttctgggat gcttctgcaa taactctaca agtattatat aagctaaaga 1501 catctaaagt atttgaaaag tcaagaaata aagatgacag gccaaacaca gacattgtag 1561 atgaagatcc atatgccatt cagatcatct cctggtgtcc agaaagtaga atgctgtgca 1621 tcgctggagt ttcagctcat gtcattattt atagattcag caagcaggaa gtaatcacag 1681 aagtcattcc gatgcttgaa gttcgattat tatatgagat aaatgatgtg gaaactccgg 1741 agggtgagca gccaccacct ttgccaacac ccgtgggagg gtccaaccct cagcccatcc 1801 ctcctcagtc tcatccatct accagtagca gttcatctga tgggcttcgt gataatgtac 1861 cttgtttaaa agttaaaaac tcaccactta aacagtctcc aggttatcaa acagaactag 1921 ttattcagtt ggtttgggtg ggtggagaac caccacaaca aataaccagc ctggcagtca 1981 attcttccta tggactggtg gtttttggca attgcaatgg cattgctatg gttgactacc 2041 tccagaaagc agtgctgctc aacctgggca ctattgaatt atatggctct aatgatcctt 2101 atcggagaga accccgatct cctcgtaaat ctcgacagcc ttcaggagcc ggtctgtgtg 2161 atattagtga agggactgtt gttccagagg atcgctgcaa atctccaacc tctgcaaaga 2221 tgtcaaggaa gttaagctta cctactgacc taaagcctga tttagatgta aaggataact 2281 cctttagccg atcacggagt tcaagtgtaa caagcattga caaagaatcc cgagaagcga 2341 tctccgctct tcatttctgt gaaacgttta ctcgaaagac ggactcgtcc ccttcccctt 2401 gtctatgggt tggaacaacg ctaggaacag tgcttgtcat tgcactgaac cttcccccag 2461 ggggagagca aagacttctt cagccagtaa ttgtgtctcc aagtggtact atattgaggt 2521 taaaaggtgc aatcttgaga atggcatttc tggataccac aggctgctta ataccacctg 2581 cgtatgaacc ctggagagag cacaatgttc ctgaagaaaa agacgaaaag gagaaattga 2641 aaaaacggcg gcctgtctca gtatccccct cctcttctca ggaaattagt gaaaaccagt 2701 atgcagtgat atgttctgaa aagcaagcaa aagtaatctc actgccaacc cagaactgtg 2761 cttataagca aaatattaca gagacctcgt ttgtgcttcg tggagatatt gtagcattga 2821 gtaacagtat ctgccttgcc tgtttctgtg ccaatggaca tataatgact tttagtttgc 2881 caagtttaag acctctgttg gatgtgtatt acttgcccct taccaatatg cggatagcca 2941 gaacgttctg ctttaccaac aatggacaag cattatacct tgtttcacct acagaaatcc 3001 agagacttac ttatagtcaa gagacctgtg aaaatcttca ggaaatgttg ggtgaactct 3061 tcactcctgt agaaacacct gaagcaccaa acaggggatt ctttaaaggc ttatttggag 3121 gtggtgcaca atctcttgac agagaagaac tatttggaga atcgtcctca ggaaaggctt 3181 caaggagcct tgcacagcat attcctggcc ctggtggcat tgaaggcgta aaaggggcag 3241 catctggagt tgttggtgaa ttagcacgag ccaggctggc actagatgaa agagggcaga 3301 aacttggcga tctggaagaa agaactgcgg ccatgttatc aagtgcagag tcgttttcta 3361 aacatgctca tgagattatg ttgaaataca aagataagaa gtggtaccag ttcttgccaa 3421 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 3481 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta