Construct: ORF ccsbBroad304_10956
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015375.1_s304c1, BRDN0000400655
- Derived from:
- ccsbBroadEn_10956
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAK (4117)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10956
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4117 | MAK | male germ cell associated k... | XM_017010866.2 | 95.9% | 96% | 1317_1317delGins55 |
| 2 | human | 4117 | MAK | male germ cell associated k... | XM_024446444.1 | 86.6% | 83.2% | (many diffs) |
| 3 | human | 4117 | MAK | male germ cell associated k... | NM_001242385.1 | 77% | 75.8% | (many diffs) |
| 4 | human | 4117 | MAK | male germ cell associated k... | XM_011514622.3 | 77% | 75.8% | (many diffs) |
| 5 | human | 4117 | MAK | male germ cell associated k... | XR_926220.3 | 73.5% | (many diffs) | |
| 6 | human | 4117 | MAK | male germ cell associated k... | XR_001743419.2 | 73.3% | (many diffs) | |
| 7 | human | 4117 | MAK | male germ cell associated k... | XM_017010865.1 | 73.3% | 69% | (many diffs) |
| 8 | human | 4117 | MAK | male germ cell associated k... | NM_005906.5 | 72.1% | 71% | (many diffs) |
| 9 | human | 4117 | MAK | male germ cell associated k... | XM_017010864.2 | 72.1% | 71% | (many diffs) |
| 10 | human | 4117 | MAK | male germ cell associated k... | NM_001242957.2 | 69.3% | 68.3% | (many diffs) |
| 11 | human | 4117 | MAK | male germ cell associated k... | XM_011514619.2 | 69.3% | 68.3% | (many diffs) |
| 12 | human | 4117 | MAK | male germ cell associated k... | XM_011514620.2 | 69.3% | 68.3% | (many diffs) |
| 13 | human | 4117 | MAK | male germ cell associated k... | XM_017010863.2 | 69.3% | 68.3% | (many diffs) |
| 14 | human | 4117 | MAK | male germ cell associated k... | XM_011514621.2 | 66% | 62.2% | (many diffs) |
| 15 | human | 4117 | MAK | male germ cell associated k... | XR_002956283.1 | 62.4% | (many diffs) | |
| 16 | human | 4117 | MAK | male germ cell associated k... | XR_926215.3 | 58.4% | (many diffs) | |
| 17 | human | 4117 | MAK | male germ cell associated k... | XM_024446443.1 | 40.3% | 39.2% | (many diffs) |
| 18 | human | 4117 | MAK | male germ cell associated k... | NR_134935.2 | 34.5% | 1_266del;1638_3972del | |
| 19 | human | 4117 | MAK | male germ cell associated k... | NR_134936.2 | 32.1% | (many diffs) | |
| 20 | human | 4117 | MAK | male germ cell associated k... | XM_011514624.2 | 29.3% | 28.3% | (many diffs) |
| 21 | mouse | 17152 | Mak | male germ cell-associated k... | XM_006516873.2 | 63.4% | 64.1% | (many diffs) |
| 22 | mouse | 17152 | Mak | male germ cell-associated k... | XM_006516872.2 | 59.4% | 60.1% | (many diffs) |
| 23 | mouse | 17152 | Mak | male germ cell-associated k... | NM_008547.2 | 59.3% | 60% | (many diffs) |
| 24 | mouse | 17152 | Mak | male germ cell-associated k... | XM_006516871.2 | 59.3% | 60% | (many diffs) |
| 25 | mouse | 17152 | Mak | male germ cell-associated k... | XM_006516870.2 | 57.5% | 58.2% | (many diffs) |
| 26 | mouse | 17152 | Mak | male germ cell-associated k... | NM_001145803.1 | 57.1% | 57.8% | (many diffs) |
| 27 | mouse | 17152 | Mak | male germ cell-associated k... | XM_006516868.2 | 57% | 57.7% | (many diffs) |
| 28 | mouse | 17152 | Mak | male germ cell-associated k... | XM_006516869.1 | 57% | 57.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1437
- ORF length:
- 1371
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccgatacaca accatgagac agttggggga cggcacgtat gggagtgtgc 121 ttatgggcaa gagtaatgaa tccggggagc tggtggccat caaaaggatg aagagaaagt 181 tctattcttg ggatgaatgc atgaacttga gagaagttaa gtctctgaag aaacttaatc 241 atgccaatgt tattaaattg aaagaagtta tcagagaaaa tgaccatctt tattttatat 301 ttgaatatat gaaagaaaac ctctatcaat taatgaaaga cagaaacaag ttgttccctg 361 aatcagtcat cagaaatatt atgtatcaaa tattgcaagg gctggctttt atccataaac 421 atggcttttt tcatagggac atgaaaccag aaaacttgct ttgtatgggt ccagagcttg 481 tgaaaattgc tgattttgga cttgcaagag aattaaggtc acagccacca tacactgatt 541 atgtatctac cagatggtat cgtgcccctg aagttttact gagatcttca gtttatagtt 601 ctcccattga tgtgtgggct gttggaagta tcatggctga actctatatg ttaaggccac 661 ttttcccagg gacaagtgag gtcgatgaaa tctttaaaat ttgccaagtt ttagggactc 721 ccaaaaaaag tgactggcca gaaggatacc agctggcatc ctctatgaac ttccgttttc 781 cccagtgtgt tcctataaac ttaaaaactc ttattcccaa tgccagtaat gaagctattc 841 agctcatgac cgaaatgttg aattgggatc caaagaaacg accgacagca agccaggcat 901 tgaaacaccc atattttcaa gttggtcagg tattaggccc ttcgtcaaat catctggaat 961 caaaacagtc tttaaataag cagctgcaac cattagaatc aaagccatct ttagttgagg 1021 tagagcctaa gcctctgccg gatataatcg atcaggttgt tggacaaccc cagccaaaaa 1081 ctagccagca gccactgcag cccattcagc cgccacagaa cctgagcgtc cagcaacctc 1141 caaagcaaca gagtcaggag aaaccgccac aaacgctatt cccgagcatc gtcaaaaaca 1201 tgccaactaa gccaaatggc acactgagtc ataaaagtgg taggaggcgt tggggtcaga 1261 ctatcttcaa gtctggagat agctgggaag agttggagga ctatgatttc ggagcctccc 1321 attccaagaa gccaagcatg ggtgttttta aagaaaaaag gaaaaaagat tctccatttc 1381 gtcaacaggt gaagatggct gttatttccc tctcagcaca ccagtttccc acgttatgcc 1441 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1501 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1561 tta