Construct: ORF ccsbBroad304_11039

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017977.1_s304c1, BRDN0000408736
Derived from:
ccsbBroadEn_11039
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PMS1 (5378)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11039
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_001128144.2 86.8% 86.8% 2008_2310del
2 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_017004348.1 86.8% 86.8% 2008_2310del
3 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_001321044.2 81.8% 81.8% 580_581ins117;1891_2193del
4 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_017004350.1 78.9% 78.9% 131_132ins183;1825_2127del
5 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_000534.4 71.7% 71.6% 1857_2342del;2494_2796del
6 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_001321045.2 71.7% 71.6% 1857_2342del;2494_2796del
7 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_001321047.2 71.7% 71.6% 1857_2342del;2494_2796del
8 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_001321048.2 71.7% 71.6% 1857_2342del;2494_2796del
9 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_024452964.1 71.7% 71.6% 1857_2342del;2494_2796del
10 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_024452965.1 71.7% 71.6% 1857_2342del;2494_2796del
11 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_001128143.2 67.5% 67.4% 580_581ins117;1740_2225del;2377_2679del
12 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_017004344.1 67.5% 67.4% 580_581ins117;1740_2225del;2377_2679del
13 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_024452966.1 67.5% 67.4% 580_581ins117;1740_2225del;2377_2679del
14 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_024452967.1 67.5% 67.4% 580_581ins117;1740_2225del;2377_2679del
15 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_001321046.2 65.2% 65.1% 131_132ins183;1674_2159del;2311_2613del
16 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_017004345.1 65.2% 65.1% 131_132ins183;1674_2159del;2311_2613del
17 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_017004346.2 65.2% 65.1% 131_132ins183;1674_2159del;2311_2613del
18 human 5378 PMS1 PMS1 homolog 1, mismatch re... XR_922951.2 64.1% (many diffs)
19 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_024452969.1 64% 64% 0_1ins528;1480_1782del
20 human 5378 PMS1 PMS1 homolog 1, mismatch re... NR_110332.2 63% (many diffs)
21 human 5378 PMS1 PMS1 homolog 1, mismatch re... XR_001738779.1 62.2% (many diffs)
22 human 5378 PMS1 PMS1 homolog 1, mismatch re... XR_002959307.1 62.1% (many diffs)
23 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_017004347.1 61% 60.9% (many diffs)
24 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_001289408.1 52.8% 52.7% 0_1ins528;1329_1814del;1966_2268del
25 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_001289409.1 52.8% 52.7% 0_1ins528;1329_1814del;1966_2268del
26 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_011511356.3 52.8% 52.7% 0_1ins528;1329_1814del;1966_2268del
27 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_024452968.1 52.8% 52.7% 0_1ins528;1329_1814del;1966_2268del
28 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_006712596.1 51.6% 50.9% (many diffs)
29 human 5378 PMS1 PMS1 homolog 1, mismatch re... XM_017004349.2 48.7% 48.6% (many diffs)
30 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_001321049.2 24.4% 21.9% (many diffs)
31 human 5378 PMS1 PMS1 homolog 1, mismatch re... NM_001321051.1 23.2% 21.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2076
ORF length:
2007
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaaacaattg cctgcggcaa cagttcgact cctttcaagt tctcagatca
121tcacttcggt ggtcagtgtt gtaaaagagc ttattgaaaa ctccttggat gctggtgcca
181caagcgtaga tgttaaactg gagaactatg gatttgataa aattgaggtg cgagataacg
241gggagggtat caaggctgtt gatgcacctg taatggcaat gaagtactac acctcaaaaa
301taaatagtca tgaagatctt gaaaatttga caacttacgg ttttcgtgga gaagccttgg
361ggtcaatttg ttgtatagct gaggttttaa ttacaacaag aacggctgct gataatttta
421gcacccagta tgttttagat ggcagtggcc acatactttc tcagaaacct tcacatcttg
481gtcaaggtac aactgtaact gctttaagat tatttaagaa tctacctgta agaaagcagt
541tttactcaac tgcaaaaaaa tgtaaagatg aaataaaaaa gatccaagat ctcctcatga
601gctttggtat ccttaaacct gacttaagga ttgtctttgt acataacaag gcagttattt
661ggcagaaaag cagagtatca gatcacaaga tggctctcat gtcagttctg gggactgctg
721ttatgaacaa tatggaatcc tttcagtacc actctgaaga atctcagatt tatctcagtg
781gatttcttcc aaagtgtgat gcagaccact ctttcactag tctttcaaca ccagaaagaa
841gtttcatctt cataaacagt cgaccagtac atcaaaaaga tatcttaaag ttaatccgac
901atcattacaa tctgaaatgc ctaaaggaat ctactcgttt gtatcctgtt ttctttctga
961aaatcgatgt tcctacagct gatgttgatg taaatttaac accagataaa agccaagtat
1021tattacaaaa taaggaatct gttttaattg ctcttgaaaa tctgatgacg acttgttatg
1081gaccattacc tagtacaaat tcttatgaaa ataataaaac agatgtttcc gcagctgaca
1141tcgttcttag taaaacagca gaaacagatg tgctttttaa taaagtggaa tcatctggaa
1201agaattattc aaatgttgat acttcagtca ttccattcca aaatgatatg cataatgatg
1261aatctggaaa aaacactgat gattgtttaa atcaccagat aagtattggt gactttggtt
1321atggtcattg tagtagtgaa atttctaaca ttgataaaaa cactaagaat gcatttcagg
1381acatttcaat gagtaatgta tcatgggaga actctcagac ggaatatagt aaaacttgtt
1441ttataagttc cgttaagcac acccagtcag aaaatggcaa taaagaccat atagatgaga
1501gtggggaaaa tgaggaagaa gcaggtcttg aaaactcttc ggaaatttct gcagatgagt
1561ggagcagggg aaatatactt aaaaattcag tgggagagaa tattgaacct gtgaaaattt
1621tagtgcctga aaaaagttta ccatgtaaag taagtaataa taattatcca atccctgaac
1681aaatgaatct taatgaagat tcatgtaaca aaaaatcaaa tgtaatagat aataaatctg
1741gaaaagttac agcttatgat ttacttagca atcgagtaat caagaaaccc atgtcagcaa
1801gtgctctttt tgttcaagat catcgtcctc agtttctcat agaaaatcct aagactagtt
1861tagaggatgc aacactacaa attgaagaac tgtggaagac attgagtgaa gaggaaaaac
1921tgaatctttt taatggatct cattatttag acgttttata taaaatgaca gcagatgacc
1981aaagatacag tggatcaact tacctgtctg atcctcgtct tacagcgaat ggtttcaaga
2041taaaattgat accaggagtt tcaattactg aaaatttgcc aactttcttg tacaaagtgg
2101ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
2161ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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