Construct: ORF ccsbBroad304_11738

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008523.1_s304c1, BRDN0000462165
Derived from:
ccsbBroadEn_11738
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BRD4 (23476)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11738
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23476 BRD4 bromodomain containing 4 NM_001330384.2 100% 100%
2 human 23476 BRD4 bromodomain containing 4 NM_014299.2 90.8% 90.8% 2159_2159delGinsCT;2161C>T;2166_2166delCins216
3 human 23476 BRD4 bromodomain containing 4 XM_011527856.3 90.8% 90.8% 2159_2159delGinsCT;2161C>T;2166_2166delCins216
4 human 23476 BRD4 bromodomain containing 4 NM_058243.2 56.5% 52.2% (many diffs)
5 human 23476 BRD4 bromodomain containing 4 XM_011527854.2 56.5% 52.2% (many diffs)
6 mouse 57261 Brd4 bromodomain containing 4 NM_198094.2 81.5% 87.4% (many diffs)
7 mouse 57261 Brd4 bromodomain containing 4 XM_006524695.3 81.4% 87.3% (many diffs)
8 mouse 57261 Brd4 bromodomain containing 4 XM_006524691.1 50.6% 50.1% (many diffs)
9 mouse 57261 Brd4 bromodomain containing 4 XM_006524690.2 50.6% 50.1% (many diffs)
10 mouse 57261 Brd4 bromodomain containing 4 NM_020508.4 49.4% 48.9% (many diffs)
11 mouse 57261 Brd4 bromodomain containing 4 XM_006524688.1 49.4% 48.9% (many diffs)
12 mouse 57261 Brd4 bromodomain containing 4 XM_006524689.2 49.4% 48.9% (many diffs)
13 mouse 57261 Brd4 bromodomain containing 4 NM_001286630.1 49.4% 48.9% (many diffs)
14 mouse 57261 Brd4 bromodomain containing 4 XM_006524683.2 49.4% 48.9% (many diffs)
15 mouse 57261 Brd4 bromodomain containing 4 XM_006524684.2 49.4% 48.9% (many diffs)
16 mouse 57261 Brd4 bromodomain containing 4 XM_006524685.1 49.4% 48.9% (many diffs)
17 mouse 57261 Brd4 bromodomain containing 4 XM_006524686.1 49.4% 48.9% (many diffs)
18 mouse 57261 Brd4 bromodomain containing 4 XM_006524687.2 49.4% 48.9% (many diffs)
19 mouse 57261 Brd4 bromodomain containing 4 XM_006524693.2 39.4% 38.7% (many diffs)
20 mouse 57261 Brd4 bromodomain containing 4 XM_011246543.2 39.4% 38.7% (many diffs)
21 mouse 57261 Brd4 bromodomain containing 4 XM_017317588.1 39.4% 38.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2451
ORF length:
2382
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtctgcggag agcggccctg ggacgagatt gagaaatctg ccagtaatgg
121gggatggact agaaacttcc caaatgtcta caacacaggc ccaggcccaa ccccagccag
181ccaacgcagc cagcaccaac cccccgcccc cagagacctc caaccctaac aagcccaaga
241ggcagaccaa ccaactgcaa tacctgctca gagtggtgct caagacacta tggaaacacc
301agtttgcatg gcctttccag cagcctgtgg atgccgtcaa gctgaacctc cctgattact
361ataagatcat taaaacgcct atggatatgg gaacaataaa gaagcgcttg gaaaacaact
421attactggaa tgctcaggaa tgtatccagg acttcaacac tatgtttaca aattgttaca
481tctacaacaa gcctggagat gacatagtct taatggcaga agctctggaa aagctcttct
541tgcaaaaaat aaatgagcta cccacagaag aaaccgagat catgatagtc caggcaaaag
601gaagaggacg tgggaggaaa gaaacaggga cagcaaaacc tggcgtttcc acggtaccaa
661acacaactca agcatcgact cctccgcaga cccagacccc tcagccgaat cctcctcctg
721tgcaggccac gcctcacccc ttccctgccg tcaccccgga cctcatcgtc cagacccctg
781tcatgacagt ggtgcctccc cagccactgc agacgccccc gccagtgccc ccccagccac
841aacccccacc cgctccagct ccccagcccg tacagagcca cccacccatc atcgcggcca
901ccccacagcc tgtgaagaca aagaagggag tgaagaggaa agcagacacc accaccccca
961ccaccattga ccccattcac gagccaccct cgctgccccc ggagcccaag accaccaagc
1021tgggccagcg gcgggagagc agccggcctg tgaaacctcc aaagaaggac gtgcccgact
1081ctcagcagca cccagcacca gagaagagca gcaaggtctc ggagcagctc aagtgctgca
1141gcggcatcct caaggagatg tttgccaaga agcacgccgc ctacgcctgg cccttctaca
1201agcctgtgga cgtggaggca ctgggcctac acgactactg tgacatcatc aagcacccca
1261tggacatgag cacaatcaag tctaaactgg aggcccgtga gtaccgtgat gctcaggagt
1321ttggtgctga cgtccgattg atgttctcca actgctataa gtacaaccct cctgaccatg
1381aggtggtggc catggcccgc aagctccagg atgtgttcga aatgcgcttt gccaagatgc
1441cggacgagcc tgaggagcca gtggtggccg tgtcctcccc ggcagtgccc cctcccacca
1501aggttgtggc cccgccctca tccagcgaca gcagcagcga tagctcctcg gacagtgaca
1561gttcgactga tgactctgag gaggagcgag cccagcggct ggctgagctc caggagcagc
1621tcaaagccgt gcacgagcag cttgcagccc tctctcagcc ccagcagaac aaaccaaaga
1681aaaaggagaa agacaagaag gaaaagaaaa aagaaaagca caaaaggaaa gaggaagtgg
1741aagagaataa aaaaagcaaa gccaaggaac ctcctcctaa aaagacgaag aaaaataata
1801gcagcaacag caatgtgagc aagaaggagc cagcgcccat gaagagcaag ccccctccca
1861cgtatgagtc ggaggaagag gacaagtgca agcctatgtc ctatgaggag aagcggcagc
1921tcagcttgga catcaacaag ctccccggcg agaagctggg ccgcgtggtg cacatcatcc
1981agtcacggga gccctccctg aagaattcca accccgacga gattgaaatc gactttgaga
2041ccctgaagcc gtccacactg cgtgagctgg agcgctatgt cacctcctgt ttgcggaaga
2101aaaggaaacc tcaagctgag aaagttgatg tgattgccgg ctcctccaag atgaagggct
2161tctcgtcctc agagtcggag agctccagtg agtccagctc ctctgacagc gaagactccg
2221aaacagcttt ctgcaccagt ggagacttcg tgtccccagg gccttccccg tatcacagtc
2281acgtgcagtg cggccgcttc agggagatgc ttcgctggtt tctggtggat gtggagcaga
2341ctgcagctgg ccagccgcat cgccagtctg ctgctggccc tgccatcacc tgggccccag
2401ccattgccta cccctcccca gagtgtgctc gttgctgtgt tggctgctcc ttgccaactt
2461tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
2521agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

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