Transcript: Mouse XM_006524693.2

PREDICTED: Mus musculus bromodomain containing 4 (Brd4), transcript variant X12, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Brd4 (57261)
Length:
6072
CDS:
836..4594

Additional Resources:

NCBI RefSeq record:
XM_006524693.2
NBCI Gene record:
Brd4 (57261)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006524693.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000088480 GCGGCAGCTAAGTCTAGATAT pLKO.1 2239 CDS 100% 13.200 10.560 N Brd4 n/a
2 TRCN0000311977 GCGGCAGCTAAGTCTAGATAT pLKO_005 2239 CDS 100% 13.200 10.560 N Brd4 n/a
3 TRCN0000088482 GCCATCTACACTACGAGAGTT pLKO.1 2374 CDS 100% 4.950 3.960 N Brd4 n/a
4 TRCN0000380416 ATGAGCACAATCAAGTCTAAA pLKO_005 1592 CDS 100% 13.200 9.240 N BRD4 n/a
5 TRCN0000349783 TGAACCTCCCTGATTACTATA pLKO_005 538 5UTR 100% 13.200 9.240 N Brd4 n/a
6 TRCN0000382028 TGAACCTCCCTGATTACTATA pLKO_005 538 5UTR 100% 13.200 9.240 N BRD4 n/a
7 TRCN0000199674 GCATCCTCAAGGAGATGTTTG pLKO.1 1470 CDS 100% 10.800 7.560 N BRD4 n/a
8 TRCN0000088479 CCTCCCTGATTACTATAAGAT pLKO.1 542 5UTR 100% 5.625 3.938 N Brd4 n/a
9 TRCN0000199972 CCAACCAAAGTCAGTTCCTTC pLKO.1 5295 3UTR 100% 4.050 2.835 N BRD4 n/a
10 TRCN0000088481 GATGTGTTTGAAATGCGCTTT pLKO.1 1736 CDS 100% 4.050 2.835 N Brd4 n/a
11 TRCN0000311976 GATGTGTTTGAAATGCGCTTT pLKO_005 1736 CDS 100% 4.050 2.835 N Brd4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006524693.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11738 pDONR223 100% 39.4% 38.7% None (many diffs) n/a
2 ccsbBroad304_11738 pLX_304 0% 39.4% 38.7% V5 (many diffs) n/a
3 TRCN0000477053 TCTAGTTTCTTACTTTTTGCCGAC pLX_317 9.5% 39.4% 38.7% V5 (many diffs) n/a
4 TRCN0000487806 ATCGTTGCGGCTGACGACGATGAT pLX_317 12.2% 39.4% 38.7% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_15013 pDONR223 71.9% 36% 7.6% None (many diffs) n/a
6 ccsbBroad304_15013 pLX_304 26.9% 36% 7.6% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000491254 CTCAGTAGTCTGCAGGTTTCGGTT pLX_317 11.4% 36% 7.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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