Construct: ORF ccsbBroad304_12273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009286.1_s304c1, BRDN0000403095
- Derived from:
- ccsbBroadEn_12273
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGD6 (55785)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12273
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55785 | FGD6 | FYVE, RhoGEF and PH domain ... | NM_018351.4 | 26.1% | 25.8% | (many diffs) |
| 2 | mouse | 13998 | Fgd6 | FYVE, RhoGEF and PH domain ... | XM_006513214.3 | 52% | 55.6% | (many diffs) |
| 3 | mouse | 13998 | Fgd6 | FYVE, RhoGEF and PH domain ... | NM_053072.3 | 23.3% | 24.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ccctcgctgt gctaatctgg ccctcaagca ctacctgctc aagccggttc 121 agaggatccc ccagtacagg ctgttgctga cagattattt gaagaatctc atagaagatg 181 ctggagatta cagagacact caagatgccc ttgctgttgt tgtagaggta gccaaccacg 241 ccaatgacac catgaagcaa ggagacaact ttcagaaact tatgcaaatt cagtacagct 301 taaatggaca ccatgaaatt gtgcagcctg gtcgggtttt tctcaaagaa ggaattctga 361 tgaagctgtc tcggaaagtg atgcaacctc gaatgttttt cctgtttaat gatgccctgc 421 tgtatacaac accagtgcag tctgggatgt ataaactgaa caacatgctc tcactggctg 481 gaatgaaggt cagaaaacct acccaagaag cctatcagaa tgaattaaag attgaaagtg 541 tagaacgttc cttcattctc tcagccagtt ctgccacaga aagggatgaa tggctagaag 601 cgatttccag ggcaatagaa gagtatgcca agaaaagaat caccttctgt cctagtagga 661 gtcttgatga ggcagactca gaaaataaag aagaagttag tcctcttgga tcgaaggctc 721 ccatctggat tcctgatacc agagccacaa tgtgtatgat ctgcacaagc gaattcactc 781 tcacctggag acgacaccac tgccgggcct gtggaaagat tgtatgccaa gcttgttcgt 841 ctaataagta tggcttagat tacctgaaaa atcaaccagc aagagtatgt gaacattgtt 901 tccaagaact gcagaaatta gatcaccagc actcccctag gattggatct cctggaaatc 961 acaaatctcc ttcaagtgcc ttatcatcag tcttacatag cattccatca gggaggaaac 1021 agaaaaaaat cccagctgct ctcaaagaag tatcagcaaa cacagaggat tcttctatga 1081 gtggctactt gtacagatca aagggcaata aaaaaccctg gaaacacttt tggtttgtca 1141 taaaaaataa agtactatat acatatgctg caagtgaggt aagatctgaa atctacccaa 1201 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1261 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta