Construct: ORF ccsbBroad304_13327
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011102.1_s304c1, BRDN0000460174
- Derived from:
- ccsbBroadEn_13327
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USP32P1 (162632)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13327
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 162632 | USP32P1 | ubiquitin specific peptidas... | NR_003190.2 | 5.4% | 1_1364del;1369G>A;1578_3901del | |
| 2 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_017025234.1 | 5.2% | 4.5% | (many diffs) |
| 3 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525379.3 | 5% | 4.4% | (many diffs) |
| 4 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524057.2 | 4.9% | 4.4% | (many diffs) |
| 5 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | NM_001304284.2 | 4.9% | 4.3% | (many diffs) |
| 6 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | NM_004505.4 | 4.9% | 4.3% | (many diffs) |
| 7 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524050.1 | 4.9% | 4.3% | (many diffs) |
| 8 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524051.2 | 4.9% | 4.3% | (many diffs) |
| 9 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524052.2 | 4.9% | 4.3% | (many diffs) |
| 10 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524053.2 | 4.9% | 4.3% | (many diffs) |
| 11 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524054.2 | 4.9% | 4.3% | (many diffs) |
| 12 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524055.2 | 4.9% | 4.3% | (many diffs) |
| 13 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524056.2 | 4.9% | 4.3% | (many diffs) |
| 14 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_017025290.1 | 4.9% | 4.3% | (many diffs) |
| 15 | human | 347716 | USP32P3 | ubiquitin specific peptidas... | NR_146076.1 | 4.6% | (many diffs) | |
| 16 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_017025233.1 | 4.3% | 3.7% | (many diffs) |
| 17 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525378.1 | 4.2% | 3.7% | (many diffs) |
| 18 | human | 220594 | USP32P2 | ubiquitin specific peptidas... | NR_003554.1 | 4.2% | (many diffs) | |
| 19 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525376.1 | 4.2% | 3.6% | (many diffs) |
| 20 | human | 84669 | USP32 | ubiquitin specific peptidas... | NM_032582.4 | 4.1% | 3.6% | (many diffs) |
| 21 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525375.1 | 4.1% | 3.6% | (many diffs) |
| 22 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525374.1 | 4.1% | 3.6% | (many diffs) |
| 23 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525373.1 | 4.1% | 3.6% | (many diffs) |
| 24 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525372.1 | 4.1% | 3.6% | (many diffs) |
| 25 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525371.1 | 4.1% | 3.6% | (many diffs) |
| 26 | mouse | 237898 | Usp32 | ubiquitin specific peptidas... | NM_001029934.1 | 3.9% | 3.4% | (many diffs) |
| 27 | mouse | 237898 | Usp32 | ubiquitin specific peptidas... | XM_011248990.1 | 3.9% | 3.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 282
- ORF length:
- 213
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagggggtg ggcagccaac cagagctggt cactcctcag gaccatgagg 121 tagctttggc caatggattc ctttatgagc atgaagcata tggcaatggc tatagcaatg 181 gtcagcttgg aaaccacagt gaagaagaca gcactgatga ccaaagagaa gacactcata 241 ttaagcctat ttatatgcaa tttcatgcca ttcaggaatt cttgccaact ttcttgtaca 301 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 361 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta