Construct: ORF ccsbBroad304_13415

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005278.1_s304c1, BRDN0000411322
Derived from:
ccsbBroadEn_13415
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CBWD5 (220869)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13415
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55871 CBWD1 COBW domain containing 1 XM_011517964.3 83.3% 80% (many diffs)
2 human 220869 CBWD5 COBW domain containing 5 XM_017014458.1 74.8% 73.1% (many diffs)
3 human 55871 CBWD1 COBW domain containing 1 XM_017014925.1 73.9% 71% (many diffs)
4 human 55871 CBWD1 COBW domain containing 1 XM_005251517.5 73.3% 70.4% (many diffs)
5 human 644019 CBWD6 COBW domain containing 6 XM_017015032.1 65.9% 64.3% (many diffs)
6 human 220869 CBWD5 COBW domain containing 5 NM_001286836.1 62.7% 62.7% 339_340ins201
7 human 220869 CBWD5 COBW domain containing 5 XM_017014455.1 61.5% 60.2% (many diffs)
8 human 220869 CBWD5 COBW domain containing 5 XR_001746239.1 59.2% 1_103del;644_912del
9 human 220869 CBWD5 COBW domain containing 5 XR_001746240.1 58.1% 1_103del;644_929del
10 human 644019 CBWD6 COBW domain containing 6 XM_017015030.1 58% 56.6% (many diffs)
11 human 220869 CBWD5 COBW domain containing 5 XR_001746238.1 57.3% 1_103del;644_941del
12 human 220869 CBWD5 COBW domain containing 5 XR_428503.2 57.1% (many diffs)
13 human 55871 CBWD1 COBW domain containing 1 XR_001746353.1 56.8% (many diffs)
14 human 445571 CBWD3 COBW domain containing 3 XR_929804.1 56.1% (many diffs)
15 human 220869 CBWD5 COBW domain containing 5 XM_006716914.1 55.9% 54.7% (many diffs)
16 human 445571 CBWD3 COBW domain containing 3 XM_006717116.1 55.8% 54.4% (many diffs)
17 human 55871 CBWD1 COBW domain containing 1 XM_011517962.3 55.2% 53.2% (many diffs)
18 human 150472 CBWD2 COBW domain containing 2 XM_011510667.1 55% 53.2% (many diffs)
19 human 150472 CBWD2 COBW domain containing 2 XR_922864.1 54.6% (many diffs)
20 human 644019 CBWD6 COBW domain containing 6 XM_017015027.1 54.2% 53% (many diffs)
21 human 445571 CBWD3 COBW domain containing 3 XR_001746298.1 53.9% (many diffs)
22 human 220869 CBWD5 COBW domain containing 5 XR_001746241.2 52.6% 1_103del;644_1025del
23 human 150472 CBWD2 COBW domain containing 2 XR_427073.2 52.5% (many diffs)
24 human 220869 CBWD5 COBW domain containing 5 NR_104600.1 51.7% (many diffs)
25 human 220869 CBWD5 COBW domain containing 5 NM_001024916.3 51.5% 50.5% (many diffs)
26 human 445571 CBWD3 COBW domain containing 3 XM_005277640.2 51.4% 50.2% (many diffs)
27 human 445571 CBWD3 COBW domain containing 3 XR_929802.1 51.4% (many diffs)
28 human 644019 CBWD6 COBW domain containing 6 XM_017015036.1 51.3% 50.2% (many diffs)
29 human 55871 CBWD1 COBW domain containing 1 XM_005251515.1 50.9% 49.1% (many diffs)
30 human 150472 CBWD2 COBW domain containing 2 XM_005263604.2 50.7% 49.1% (many diffs)
31 human 644019 CBWD6 COBW domain containing 6 XR_001746362.1 50% (many diffs)
32 human 220869 CBWD5 COBW domain containing 5 XM_005272751.1 48.9% 47.9% (many diffs)
33 human 445571 CBWD3 COBW domain containing 3 XM_005277639.2 48.8% 47.6% (many diffs)
34 human 644019 CBWD6 COBW domain containing 6 XM_024447653.1 48.7% 47.6% (many diffs)
35 human 150472 CBWD2 COBW domain containing 2 XR_244814.2 48.3% (many diffs)
36 human 55871 CBWD1 COBW domain containing 1 XM_005251514.1 48.2% 46.6% (many diffs)
37 human 150472 CBWD2 COBW domain containing 2 NM_001330337.1 48% 46.6% (many diffs)
38 human 644019 CBWD6 COBW domain containing 6 XR_001746363.1 48% (many diffs)
39 human 220869 CBWD5 COBW domain containing 5 NM_001363751.1 47.6% 46.7% (many diffs)
40 human 445571 CBWD3 COBW domain containing 3 XM_005277637.2 47.5% 46.4% (many diffs)
41 human 644019 CBWD6 COBW domain containing 6 XM_017015026.1 47.4% 46.4% (many diffs)
42 human 644019 CBWD6 COBW domain containing 6 XM_017015035.1 47.4% 46.4% (many diffs)
43 human 55871 CBWD1 COBW domain containing 1 NM_001145356.1 46.9% 45.3% (many diffs)
44 human 150472 CBWD2 COBW domain containing 2 NM_001330336.1 46.8% 45.3% (many diffs)
45 human 445571 CBWD3 COBW domain containing 3 XR_929803.1 46% (many diffs)
46 human 644019 CBWD6 COBW domain containing 6 XR_001746359.1 45.8% (many diffs)
47 human 220869 CBWD5 COBW domain containing 5 NM_001330668.1 45.3% 44.4% (many diffs)
48 human 445571 CBWD3 COBW domain containing 3 NM_201453.3 45.2% 44.2% (many diffs)
49 human 644019 CBWD6 COBW domain containing 6 NM_001085457.1 45.1% 44.2% (many diffs)
50 human 55871 CBWD1 COBW domain containing 1 NM_018491.3 44.7% 43.2% (many diffs)
51 human 150472 CBWD2 COBW domain containing 2 NM_172003.3 44.5% 43.2% (many diffs)
52 human 644019 CBWD6 COBW domain containing 6 XM_017015025.1 43.9% 43% (many diffs)
53 human 445571 CBWD3 COBW domain containing 3 XM_017014730.1 43.4% 42.5% (many diffs)
54 human 644019 CBWD6 COBW domain containing 6 XM_017015024.1 43.4% 42.5% (many diffs)
55 human 644019 CBWD6 COBW domain containing 6 XM_017015023.1 42.2% 41.4% (many diffs)
56 human 644019 CBWD6 COBW domain containing 6 XM_017015022.1 41.7% 40.8% (many diffs)
57 human 445571 CBWD3 COBW domain containing 3 XR_001746301.1 41.3% 1_166del;605G>A;707_1305del
58 human 445571 CBWD3 COBW domain containing 3 XR_001746304.1 41.2% 1_166del;605G>A;707_1308del
59 human 644019 CBWD6 COBW domain containing 6 XR_001746361.1 40.9% (many diffs)
60 human 644019 CBWD6 COBW domain containing 6 XM_017015021.1 40.6% 39.8% (many diffs)
61 human 445571 CBWD3 COBW domain containing 3 NM_001291821.1 40.2% 39.4% (many diffs)
62 human 220869 CBWD5 COBW domain containing 5 XM_005272750.1 40.2% 39.4% (many diffs)
63 human 644019 CBWD6 COBW domain containing 6 XM_017015020.1 40.2% 39.4% (many diffs)
64 human 55871 CBWD1 COBW domain containing 1 XM_005251513.4 39.8% 38.4% (many diffs)
65 human 644019 CBWD6 COBW domain containing 6 XM_017015019.1 38.7% 38% (many diffs)
66 human 445571 CBWD3 COBW domain containing 3 XR_001746303.1 38.6% 1_166del;605G>A;707_1393del
67 human 445571 CBWD3 COBW domain containing 3 XR_001746302.1 37.7% 1_166del;605G>A;707_1428del
68 human 55871 CBWD1 COBW domain containing 1 XM_011517961.2 35.8% 32.5% (many diffs)
69 human 55871 CBWD1 COBW domain containing 1 NM_001145355.1 34.1% 31% (many diffs)
70 human 150472 CBWD2 COBW domain containing 2 NM_001330339.1 34% 31.2% (many diffs)
71 human 220869 CBWD5 COBW domain containing 5 XR_002956768.1 32.2% 1_103del;644_1674del
72 human 220869 CBWD5 COBW domain containing 5 XR_001746237.2 31.4% 1_103del;644_1717del
73 human 445571 CBWD3 COBW domain containing 3 XR_002956784.1 31% 1_166del;605G>A;707_1737del
74 human 220869 CBWD5 COBW domain containing 5 XR_002956769.1 30.9% 1_103del;644_1747del
75 human 220869 CBWD5 COBW domain containing 5 XR_428504.1 29.9% 1_103del;644_1804del
76 human 220869 CBWD5 COBW domain containing 5 XR_001746234.1 29.8% (many diffs)
77 human 220869 CBWD5 COBW domain containing 5 XR_001746232.1 29% (many diffs)
78 human 445571 CBWD3 COBW domain containing 3 XR_001746299.1 28.8% 1_166del;605G>A;707_1867del
79 human 55871 CBWD1 COBW domain containing 1 XR_001746352.1 28.7% (many diffs)
80 human 445571 CBWD3 COBW domain containing 3 XR_001746297.1 28.1% (many diffs)
81 human 150472 CBWD2 COBW domain containing 2 NR_138473.1 27.6% (many diffs)
82 human 445571 CBWD3 COBW domain containing 3 XR_001746300.1 27% 1_166del;605G>A;707_1990del
83 human 150472 CBWD2 COBW domain containing 2 NR_138474.1 26.2% (many diffs)
84 human 644019 CBWD6 COBW domain containing 6 XR_001746360.1 25% (many diffs)
85 human 150472 CBWD2 COBW domain containing 2 XR_002959242.1 18.2% (many diffs)
86 human 220869 CBWD5 COBW domain containing 5 XM_017014457.1 15.3% 14.6% (many diffs)
87 human 150472 CBWD2 COBW domain containing 2 XM_024452717.1 14.4% 13.7% (many diffs)
88 human 220869 CBWD5 COBW domain containing 5 XR_002956766.1 12% (many diffs)
89 human 220869 CBWD5 COBW domain containing 5 XR_001746233.1 11.9% (many diffs)
90 human 220869 CBWD5 COBW domain containing 5 XR_001746235.1 11.8% (many diffs)
91 human 220869 CBWD5 COBW domain containing 5 XR_001746231.1 11.7% (many diffs)
92 human 220869 CBWD5 COBW domain containing 5 XR_002956767.1 10.4% (many diffs)
93 mouse 226043 Cbwd1 COBW domain containing 1 NM_146097.3 39.2% 37.4% (many diffs)
94 mouse 226043 Cbwd1 COBW domain containing 1 NR_033744.1 23% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
606
ORF length:
540
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt accggctgtt ggatctgtgg atgaggaaga ggatcctgcg gaggaggatt
121gtcctgaatt ggttcccatt gagacgacgc aaagcgagga ggaggaaaag tctggcctcg
181gcgccaagat cccagtcaca attatcaccg ggtatttagg tgctgggaag acaacacttc
241tgaactatat tttgacagag caacatagta aaagagtagc ggtcatttta aatgaatctg
301gggaaggaag tgcgctggag aaatccttag ctgtcagcca aggtggagag ctctatgaag
361agtggctgga acttagaaac ggttgcctct gctgttcagt gaaggacaat ggccttagag
421ctattgagaa tttgatgcaa aagaagggga aatttgatga catactgtta gagaccactg
481gattagcaga ccctggtgca gtgacttcta tgttttgggt tgatgctgaa ttagggagtg
541atatttacct tgatggtatc ataactattg tggattcaaa atatggatta aaagtgaaat
601accactaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
661gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
721acctctggat ta

Download FASTA (ORF) (Full)