Construct: ORF ccsbBroad304_13656

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008357.1_s304c1, BRDN0000460699
Derived from:
ccsbBroadEn_13656
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPDYE8P (389517)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13656
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100996746 SPDYE11 speedy/RINGO cell cycle reg... NM_001351349.1 100% 100%
2 human 102723849 SPDYE17 speedy/RINGO cell cycle reg... NM_001351351.1 100% 100%
3 human 100505767 SPDYE18 speedy/RINGO cell cycle reg... NM_001351348.1 87.6% 78.5% (many diffs)
4 human 100996746 SPDYE11 speedy/RINGO cell cycle reg... XM_024446630.1 84.3% 77.5% 737_858del;918_942del
5 human 102723555 SPDYE16 speedy/RINGO cell cycle reg... NM_001351597.1 73.7% 61.8% (many diffs)
6 human 102723555 SPDYE16 speedy/RINGO cell cycle reg... XM_017012887.2 73.7% 61.8% (many diffs)
7 human 285955 SPDYE1 speedy/RINGO cell cycle reg... XM_017012016.2 73.6% 64.1% (many diffs)
8 human 388333 SPDYE4 speedy/RINGO cell cycle reg... NM_001128076.3 72.2% 62.2% (many diffs)
9 human 388333 SPDYE4 speedy/RINGO cell cycle reg... XM_005256633.4 72.2% 62.2% (many diffs)
10 human 285955 SPDYE1 speedy/RINGO cell cycle reg... NM_175064.2 67.3% 56.1% (many diffs)
11 human 285955 SPDYE1 speedy/RINGO cell cycle reg... XM_005249719.3 65.1% 59.3% (many diffs)
12 human 285955 SPDYE1 speedy/RINGO cell cycle reg... XM_017012015.1 65.1% 59.3% (many diffs)
13 human 441273 SPDYE2 speedy/RINGO cell cycle reg... NM_001031618.3 61% 55.9% (many diffs)
14 human 100310812 SPDYE2B speedy/RINGO cell cycle reg... NM_001166339.1 61% 55.9% (many diffs)
15 human 100310812 SPDYE2B speedy/RINGO cell cycle reg... XM_005250093.4 61% 55.9% (many diffs)
16 human 441273 SPDYE2 speedy/RINGO cell cycle reg... XM_017012223.1 61% 55.9% (many diffs)
17 human 729597 SPDYE6 speedy/RINGO cell cycle reg... NM_001146210.4 60.9% 55.9% (many diffs)
18 human 442590 SPDYE5 speedy/RINGO cell cycle reg... NM_001306141.1 60.8% 55.9% (many diffs)
19 human 441251 SPDYE7P speedy/RINGO cell cycle reg... NR_003666.2 59.3% (many diffs)
20 human 285955 SPDYE1 speedy/RINGO cell cycle reg... XM_017012017.2 58.7% 53% (many diffs)
21 human 100310812 SPDYE2B speedy/RINGO cell cycle reg... XM_011515702.3 57.1% 51.1% (many diffs)
22 human 441273 SPDYE2 speedy/RINGO cell cycle reg... XM_011516237.3 57.1% 51.1% (many diffs)
23 human 441272 SPDYE3 speedy/RINGO cell cycle reg... NM_001004351.5 44.3% 43.2% (many diffs)
24 human 441272 SPDYE3 speedy/RINGO cell cycle reg... XM_011516234.3 40.7% 36.6% (many diffs)
25 human 441272 SPDYE3 speedy/RINGO cell cycle reg... XM_011516235.3 40.7% 36.6% (many diffs)
26 human 641776 SPDYE14P speedy/RINGO cell cycle reg... NR_146072.1 34% 1_200del;319T>C;996_2332del
27 human 105180390 SPDYE13P speedy/RINGO cell cycle reg... NR_146073.1 34% 1_200del;319T>C;996_2332del
28 human 105180391 SPDYE15P speedy/RINGO cell cycle reg... NR_146074.1 34% 1_200del;319T>C;996_2332del
29 human 728524 SPDYE8P speedy/RINGO cell cycle reg... NR_003664.2 27.5% 1_571del;1367_2884del
30 human 643862 SPDYE10P speedy/RINGO cell cycle reg... NR_146079.1 27.2% 1_1155del;1341A>G;1951_2918del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
864
ORF length:
795
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggacagatt ttgggaaaga tcatgatgag ccatcaaccg cagccccagg
121aagagcggag cccccagcgg agcacctcag ggtaccccct ccaggaggtg gtggatgatg
181aagtgtcggg accatcagcc cctggggtag atcccagccc cccacgtagg tcccttggct
241ggaaaaggaa gagggaatgt ttggatgaat ctgatgatga gccagagaag gagctcgccc
301ctgagcctga ggagacctgg gtggcggaga cgctgtgtgg cctcaagatg aaggcgaagc
361gacggcgagt gtcgctcgtg ctccctgagt actacgaggc cttcaacagg ctgcttgagg
421atcctgtcat taaaagactc ctggcctggg acaaagatct gagggtgtcg gacaagtatc
481tcctggctat ggtcatagcg tatttcagcc gggccggcct cccctcctgg caataccaac
541gcattcattt cttcctggct ctctatctgg ccaatgacat ggaggaggac gacgaggccc
601ccaaacaaaa catcttctac ttcctgtacg aggagacccg ctctcatata cccttgctca
661gtgagctttg gttccagtta tgccgttaca tgaacccgag ggccaggaag aactgctctc
721agatagcctt gttccggaag tatcggttcc acttcttttg ttccatgcgc tgcagggctt
781gggtttccct ggaggagttg gaagagatcc aggcttatga cccagagcac tgggtgtggg
841cgcgagatcg cgcccacctt tccttgccaa ctttcttgta caaagtggtt ggtaagccta
901tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
961gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)