Construct: ORF ccsbBroad304_13848
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007939.1_s304c1, BRDN0000462255
- Derived from:
- ccsbBroadEn_13848
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGFR (1956)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13848
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346897.2 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346898.2 | 95.9% | 95.9% | (many diffs) |
| 3 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346899.1 | 92.2% | 89.7% | (many diffs) |
| 4 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.5 | 88.8% | 86.5% | (many diffs) |
| 5 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2239_2247del | 88.6% | 86.2% | (many diffs) |
| 6 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2240_2254del | 88.5% | 86.1% | (many diffs) |
| 7 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346900.2 | 84.5% | 82.1% | (many diffs) |
| 8 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346941.2 | 73.3% | 70.8% | (many diffs) |
| 9 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201284.2 | 56.2% | 50.1% | (many diffs) |
| 10 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201282.2 | 51.2% | 51.2% | (many diffs) |
| 11 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201283.1 | 31.6% | 31.5% | (many diffs) |
| 12 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_207655.2 | 76.3% | 79.1% | (many diffs) |
| 13 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_007912.4 | 44.8% | 46.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3342
- ORF length:
- 3273
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgaccctcc gggacggccg gggcagcgct cctggcgctg ctggctgcgc 121 tctgcccggc gagtcgggct ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc 181 tcacgcagtt gggcactttt gaagatcatt ttctcagcct ccagaggatg ttcaataact 241 gtgaggtggt ccttgggaat ttggaaatta cctatgtgca gaggaattat gatctttcct 301 tcttaaagac catccaggag gtggctggtt atgtcctcat tgccctcaac acagtggagc 361 gaattccttt ggaaaacctg cagatcatca gaggaaatat gtactacgaa aattcctatg 421 ccttagcagt cttatctaac tatgatgcaa ataaaaccgg actgaaggag ctgcccatga 481 gaaatttaca tggacaaaag tgtgatccaa gctgtcccaa tgggagctgc tggggtgcag 541 gagaggagaa ctgccagaaa ctgaccaaaa tcatctgtgc ccagcagtgc tccgggcgct 601 gccgtggcaa gtcccccagt gactgctgcc acaaccagtg tgctgcaggc tgcacaggcc 661 cccgggagag cgactgcctg gtctgccgca aattccgaga cgaagccacg tgcaaggaca 721 cctgcccccc actcatgctc tacaacccca ccacgtacca gatggatgtg aaccccgagg 781 gcaaatacag ctttggtgcc acctgcgtga agaagtgtcc ccgtaattat gtggtgacag 841 atcacggctc gtgcgtccga gcctgtgggg ccgacagcta tgagatggag gaagacggcg 901 tccgcaagtg taagaagtgc gaagggcctt gccgcaaagt gtgtaacgga ataggtattg 961 gtgaatttaa agactcactc tccataaatg ctacgaatat taaacacttc aaaaactgca 1021 cctccatcag tggcgatctc cacatcctgc cggtggcatt taggggtgac tccttcacac 1081 atactcctcc tctggatcca caggaactgg atattctgaa aaccgtaaag gaaatcacag 1141 ggtttttgct gattcaggct tggcctgaaa acaggacgga cctccatgcc tttgagaacc 1201 tagaaatcat acgcggcagg accaagcaac atggtcagtt ttctcttgca gtcgtcagcc 1261 tgaacataac atccttggga ttacgctccc tcaaggagat aagtgatgga gatgtgataa 1321 tttcaggaaa caaaaatttg tgctatgcaa atacaataaa ctggaaaaaa ctgtttggga 1381 cctccggtca gaaaaccaaa attataagca acagaggtga aaacagctgc aaggccacag 1441 gccaggtctg ccatgccttg tgctcccccg agggctgctg gggcccggag cccagggact 1501 gcgtctcttg ccggaatgtc agccgaggca gggaatgcgt ggacaagtgc aaccttctgg 1561 agggtgagcc aagggagttt gtggagaact ctgagtgcat acagtgccac ccagagtgcc 1621 tgcctcaggc catgaacatc acctgcacag gacggggacc agacaactgt atccagtgtg 1681 cccactacat tgacggcccc cactgcgtca agacctgccc ggcaggagtc atgggagaaa 1741 acaacaccct ggtctggaag tacgcagacg ccggccatgt gtgccacctg tgccatccaa 1801 actgcaccta cggatgcact gggccaggtc ttgaaggctg tccaacgaat gggcctaaga 1861 tcccgtccat cgccactggg atggtggggg ccctcctctt gctgctggtg gtggccctgg 1921 ggatcggcct cttcatgcga aggcgccaca tcgttcggaa gcgcacgctg cggaggctgc 1981 tgcaggagag ggagcttgtg gagcctctta cacccagtgg agaagctccc aaccaagctc 2041 tcttgaggat cttgaaggaa actgaattca aaaagatcaa agtgctgggc tccggtgcgt 2101 tcggcacggt gtataaggga ctctggatcc cagaaggtga gaaagttaaa attcccgtcg 2161 ctatcaagga attaagagaa gcaacatctc cgaaagccaa caaggaaatc ctcgatgaag 2221 cctacgtgat ggccagcgtg gacaaccccc acgtgtgccg cctgctgggc atctgcctca 2281 cctccaccgt gcagctcatc acgcagctca tgcccttcgg ctgcctcctg gactatgtcc 2341 gggaacacaa agacaatatt ggctcccagt acctgctcaa ctggtgtgtg cagatcgcaa 2401 agggcatgaa ctacttggag gaccgtcgct tggtgcaccg cgacctggca gccaggaacg 2461 tactggtgaa aacaccgcag catgtcaaga tcacagattt tgggctggcc aaactgctgg 2521 gtgcggaaga gaaagaatac catgcagaag gaggcaaagt gcctatcaag tggatggcat 2581 tggaatcaat tttacacaga atctataccc accagagtga tgtctggagc tacggggtga 2641 ccgtttggga gttgatgacc tttggatcca agccatatga cggaatccct gccagcgaga 2701 tctcctccat cctggagaaa ggagaacgcc tccctcagcc acccatatgt accatcgatg 2761 tctacatgat catggtcaag tgctggatga tagacgcaga tagtcgccca aagttccgtg 2821 agttgatcat cgaattctcc aaaatggccc gagaccccca gcgctacctt gtcattcagg 2881 gggatgaaag aatgcatttg ccaagtccta cagactccaa cttctaccgt gccctgatgg 2941 atgaagaaga catggacgac gtggtggatg ccgacgagta cctcatccca cagcagggct 3001 tcttcagcag cccctccacg tcacggactc ccctcctgag ctctctgagt gcaaccagca 3061 acaattccac cgtggcttgc attgatagaa atgggctgca aagctgtccc atcaaggaag 3121 acagcttctt gcagcgatac agctcagacc ccacaggcgc cttgactgag gacagcatag 3181 acgacacctt cctcccagtg cctggtgagt ggcttgtctg gaaacagtcc tgctcctcaa 3241 cctcctcgac ccactcagca gcagccagtc tccagtgtcc aagccaggtg ctccctccag 3301 catctccaga gggggaaaca gtggcagatt tccagacaca gttgccaact ttcttgtaca 3361 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 3421 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta