Construct: ORF ccsbBroad304_14174
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005537.1_s304c1, BRDN0000404906
- Derived from:
- ccsbBroadEn_14174
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CDKAL1 (54901)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14174
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_017010986.1 | 21.7% | 2% | 14_17delCCTG;285_286insGGT;289_1305del |
| 2 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_011514719.2 | 21.3% | 2% | 14_17delCCTG;285_286insGGT;289_1329del |
| 3 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_011514718.1 | 16.9% | 1.6% | 14_17delCCTG;285_286insGGT;289_1674del |
| 4 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | NM_017774.3 | 16.3% | 1.5% | 14_17delCCTG;285_286insGGT;289_1737del |
| 5 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_024446481.1 | 15.9% | .8% | 14_17delCCTG;285_286insGGT;289_1779del |
| 6 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743501.1 | 9.3% | (many diffs) | |
| 7 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743500.1 | 9.1% | (many diffs) | |
| 8 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_926267.2 | 7.6% | (many diffs) | |
| 9 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_926265.2 | 7.4% | (many diffs) | |
| 10 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743495.2 | 7.4% | (many diffs) | |
| 11 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_926266.2 | 7.2% | (many diffs) | |
| 12 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743496.2 | 6.7% | (many diffs) | |
| 13 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | NM_001308486.1 | 60.3% | 1.9% | (many diffs) |
| 14 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | XM_011244342.2 | 19% | 2% | (many diffs) |
| 15 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | NM_144536.3 | 14.2% | .6% | (many diffs) |
| 16 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | NR_131896.1 | 7.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 150
- ORF length:
- 84
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc ttctgcattg atacactact ggatgacatc gaagatatcg tgtctcagga 121 agattcaaaa ccacaagata ggcattttgt aagaaaggat gttgtcccga aggtacgaag 181 gcgaaatacc caaaaatatt tgcaagagga agaaaacagt ccaccaagtg acagcactat 241 tccaggcata cagaaaattt ggatacgaac atggggttgt tctcataata attcagatgg 301 agaatatatg gctggacagc tagctgctta tggctataaa attacaggtg aatgcccaac 361 tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac 421 gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggatta