Construct: ORF ccsbBroad304_14514

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001978.1_s304c1, BRDN0000460529
Derived from:
ccsbBroadEn_14514
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LOC644936 (644936)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14514
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 60 ACTB actin beta NM_001101.5 51.7% 45% (many diffs)
2 human 71 ACTG1 actin gamma 1 NM_001199954.2 50% 45% (many diffs)
3 human 71 ACTG1 actin gamma 1 NM_001614.5 50% 45% (many diffs)
4 human 644936 LOC644936 actin beta pseudogene NR_004845.2 47% 1_141del;789delC;799_1394del
5 human 71 ACTG1 actin gamma 1 NR_037688.3 31.7% (many diffs)
6 human 653781 POTEJ POTE ankyrin domain family ... XM_017004743.2 29.1% 24.9% (many diffs)
7 human 653269 POTEI POTE ankyrin domain family ... XM_017004734.2 28.9% 24.5% (many diffs)
8 human 728378 POTEF POTE ankyrin domain family ... XM_017004835.1 28.8% 24.3% (many diffs)
9 human 653781 POTEJ POTE ankyrin domain family ... XM_017004742.1 28.3% 24.2% (many diffs)
10 human 653269 POTEI POTE ankyrin domain family ... XM_017004733.1 28.2% 23.8% (many diffs)
11 human 728378 POTEF POTE ankyrin domain family ... XM_017004834.1 28% 23.7% (many diffs)
12 human 445582 POTEE POTE ankyrin domain family ... XM_011511216.2 19.8% 16.6% (many diffs)
13 human 445582 POTEE POTE ankyrin domain family ... XM_017004159.2 19.6% 16.4% (many diffs)
14 human 653781 POTEJ POTE ankyrin domain family ... NM_001277083.2 18.4% 15.7% (many diffs)
15 human 653781 POTEJ POTE ankyrin domain family ... XM_011511683.2 18.2% 15.6% (many diffs)
16 human 653781 POTEJ POTE ankyrin domain family ... XM_017004741.2 18.2% 15.6% (many diffs)
17 human 653269 POTEI POTE ankyrin domain family ... NM_001277406.2 17.7% 14.9% (many diffs)
18 human 653269 POTEI POTE ankyrin domain family ... XM_024453046.1 17.7% 14.9% (many diffs)
19 human 445582 POTEE POTE ankyrin domain family ... NM_001083538.2 17.6% 14.7% (many diffs)
20 human 445582 POTEE POTE ankyrin domain family ... XM_017004161.2 17.6% 14.7% (many diffs)
21 human 728378 POTEF POTE ankyrin domain family ... NM_001099771.2 17.6% 14.8% (many diffs)
22 human 653269 POTEI POTE ankyrin domain family ... XM_011511670.2 17.5% 14.8% (many diffs)
23 human 653269 POTEI POTE ankyrin domain family ... XM_017004732.2 17.5% 14.8% (many diffs)
24 human 728378 POTEF POTE ankyrin domain family ... XM_017004833.1 17.4% 14.7% (many diffs)
25 human 653269 POTEI POTE ankyrin domain family ... NM_001371926.1 17.3% 14.6% (many diffs)
26 mouse 11461 Actb actin, beta NM_007393.5 49.4% 45% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
726
ORF length:
657
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggggtcacca acactgtgcc catctatgaa gggtacaccc ctcccccaca
121ccatcctgtg tctggacctg actggccgga acctgactga ctacctcatg aagatcctca
181cccagtgtgg ctacagcttc accaccacag tcatgcagga aattgtgtgt gacatcaaga
241aaaggctgtg ctacatcccc ctggactttg agcaggagat ggccatggtg ggctccagct
301cctccctgga gaagagctac aagctgccca atggccaggt catcaccatt agcaacaagt
361ggttctgctg ccccgaggca ctcttccaaa cttccttcgt gggcatggaa tcctgtggca
421tccacgaaac taccttcaac tccatcatga agtcggatgt ggacatctac aaagacctgt
481atgccaacac agtgctgtct ggcagcacca ccatgtaccc tagcatcacc aacaggatgc
541agaaggagat taccgccctg gcgcccagcg caatgaagat caagatcatt gctcctcctg
601agtgcaagta ctctgtgtgg atcagaggct ccatcctggc ctcgctgtcc accttccagc
661agatgtggat cagcaagcag gagtacagtg agtccagccc ctccatcgtc caccgaaatg
721cttcttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc ctctcctcgg
781tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa
841cctctggatt a

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