Transcript: Human XM_017004734.2

PREDICTED: Homo sapiens POTE ankyrin domain family member I (POTEI), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
POTEI (653269)
Length:
2624
CDS:
80..2053

Additional Resources:

NCBI RefSeq record:
XM_017004734.2
NBCI Gene record:
POTEI (653269)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017004734.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000254475 CAATGGTGATGATGGATTAAT pLKO_005 145 CDS 100% 15.000 7.500 Y POTEC n/a
2 TRCN0000262863 TTGGCTTGACTCAGGATTTAA pLKO_005 2173 3UTR 100% 15.000 7.500 Y POTEF n/a
3 TRCN0000141790 GCAACCCAGAACAAGACTTAA pLKO.1 297 CDS 100% 13.200 6.600 Y POTEE n/a
4 TRCN0000145107 GCAATGGTGATGATGGATTAA pLKO.1 144 CDS 100% 13.200 6.600 Y POTEE n/a
5 TRCN0000148153 GCAATGGTGATGATGGATTAA pLKO.1 144 CDS 100% 13.200 6.600 Y POTED n/a
6 TRCN0000150907 GAAGAACAGAACACTGGAATA pLKO.1 638 CDS 100% 10.800 5.400 Y POTEG n/a
7 TRCN0000117346 CATCACCAACTGGGATGACAT pLKO.1 1150 CDS 100% 4.950 2.475 Y ACTA1 n/a
8 TRCN0000144012 CCAGTTACTTTCTGACTACAA pLKO.1 3 5UTR 100% 4.950 2.475 Y POTEE n/a
9 TRCN0000150726 CCAGTTACTTTCTGACTACAA pLKO.1 3 5UTR 100% 4.950 2.475 Y POTEG n/a
10 TRCN0000029409 CGAGAAGATGACCCAGATCAT pLKO.1 1273 CDS 100% 4.950 2.475 Y ACTB n/a
11 TRCN0000276219 CGAGAAGATGACCCAGATCAT pLKO_005 1273 CDS 100% 4.950 2.475 Y ACTB n/a
12 TRCN0000090901 CGTGCGTGACATCAAAGAGAA pLKO.1 1549 CDS 100% 4.950 2.475 Y Actb n/a
13 TRCN0000309123 CGTGCGTGACATCAAAGAGAA pLKO_005 1549 CDS 100% 4.950 2.475 Y Actb n/a
14 TRCN0000117202 GACTCTGACTTAGTTGCGTTA pLKO.1 2057 3UTR 100% 4.050 2.025 Y POTEKP n/a
15 TRCN0000090902 CCAGATCATGTTTGAGACCTT pLKO.1 1285 CDS 100% 2.640 1.320 Y Actb n/a
16 TRCN0000117205 CCCTCCATTGTCCACCGCAAA pLKO.1 2024 CDS 100% 1.350 0.675 Y POTEKP n/a
17 TRCN0000089842 TGTGCTATGTTGCCCTGGATT pLKO.1 1572 CDS 100% 4.950 2.475 Y Actg-ps1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017004734.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05763 pDONR223 100% 52.6% 51.9% None (many diffs) n/a
2 ccsbBroad304_05763 pLX_304 53.1% 52.6% 51.9% V5 (many diffs) n/a
3 TRCN0000470279 TCAAGCCTGGAACGGGCTCACGTC pLX_317 31.7% 52.6% 51.9% V5 (many diffs) n/a
4 ccsbBroadEn_15351 pDONR223 0% 50.3% 51.7% None (many diffs) n/a
5 ccsbBroad304_15351 pLX_304 0% 50.3% 51.7% V5 (many diffs) n/a
6 ccsbBroadEn_13808 pDONR223 100% 50.3% 51.4% None (many diffs) n/a
7 ccsbBroad304_13808 pLX_304 0% 50.3% 51.4% V5 (not translated due to frame shift) (many diffs) n/a
8 TRCN0000471742 AATGGGAGTTCTCACGTCAGGTCC pLX_317 44.8% 50.3% 51.4% V5 (not translated due to frame shift) (many diffs) n/a
9 ccsbBroadEn_05764 pDONR223 100% 50.3% 51.7% None (many diffs) n/a
10 ccsbBroad304_05764 pLX_304 0% 50.3% 51.7% V5 (many diffs) n/a
11 TRCN0000466781 AATTGTGGTGCTTGTTGCCGCCTG pLX_317 31.7% 50.3% 51.7% V5 (many diffs) n/a
12 ccsbBroadEn_00015 pDONR223 100% 47.4% 49.4% None (many diffs) n/a
13 ccsbBroad304_00015 pLX_304 0% 47.4% 49.4% V5 (many diffs) n/a
14 TRCN0000468012 TCTATATTCTTCCCTCACTCATAA pLX_317 37.9% 47.4% 49.4% V5 (many diffs) n/a
15 ccsbBroadEn_00014 pDONR223 100% 46.9% 49.3% None (many diffs) n/a
16 ccsbBroad304_00014 pLX_304 0% 46.9% 49.3% V5 (not translated due to prior stop codon) (many diffs) n/a
17 TRCN0000467679 GCTACTGCTTTGAGGGTTATAACT pLX_317 37.9% 46.9% 49.3% V5 (not translated due to prior stop codon) (many diffs) n/a
18 ccsbBroadEn_14514 pDONR223 100% 28.9% 24.5% None (many diffs) n/a
19 ccsbBroad304_14514 pLX_304 0% 28.9% 24.5% V5 (not translated due to frame shift) (many diffs) n/a
20 TRCN0000481436 GCTCGTTTACTAGTATTTTTAATC pLX_317 64.2% 28.9% 24.5% V5 (not translated due to frame shift) (many diffs) n/a
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