Transcript: Human NM_001350717.2

Homo sapiens diacylglycerol kinase beta (DGKB), transcript variant 12, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
DGKB (1607)
Length:
6811
CDS:
351..2729

Additional Resources:

NCBI RefSeq record:
NM_001350717.2
NBCI Gene record:
DGKB (1607)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146143 AGAGGCAGAATCGCAACTGG pXPR_003 AGG 1515 64% 18 0.345 DGKB DGKB 76918
2 BRDN0001148085 TGAACATGCTGATTGGCGTG pXPR_003 GGG 801 34% 10 -0.0341 DGKB DGKB 76917
3 BRDN0001145601 GAAAGACTGTAAACCTGACG pXPR_003 AGG 1362 57% 16 -0.3291 DGKB DGKB 76916
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001350717.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196692 GAGGATAAGCTTGAGTTTATG pLKO.1 801 CDS 100% 13.200 18.480 N DGKB n/a
2 TRCN0000199895 GACGAAGCCATCGACGAATAG pLKO.1 2353 CDS 100% 10.800 15.120 N DGKB n/a
3 TRCN0000000768 GCAAATATACACAGGCCTGAA pLKO.1 2495 CDS 100% 4.050 5.670 N DGKB n/a
4 TRCN0000194940 CCTGAAGTGATACTGCATATT pLKO.1 2946 3UTR 100% 13.200 10.560 N DGKB n/a
5 TRCN0000000764 CCTGAACTGCATGATTTATTA pLKO.1 4184 3UTR 100% 15.000 10.500 N DGKB n/a
6 TRCN0000000766 CCTGCAAGAAGCTACATGAAT pLKO.1 2209 CDS 100% 5.625 3.938 N DGKB n/a
7 TRCN0000000765 CCTAATGACAAAGATGAGAAA pLKO.1 2016 CDS 100% 4.950 3.465 N DGKB n/a
8 TRCN0000000767 CCTTTATGACACGGATGGGAA pLKO.1 827 CDS 100% 2.640 1.848 N DGKB n/a
9 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 6123 3UTR 100% 4.950 2.475 Y ERAP2 n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 6124 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001350717.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14607 pDONR223 68.3% 96.5% 29.4% None (many diffs) n/a
2 ccsbBroad304_14607 pLX_304 0% 96.5% 29.4% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000473795 GATAATAGTCGCCATTAGCGACGG pLX_317 16.3% 96.5% 29.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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